Anthocyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits

Anthocyanins are colored water-soluble pigments belonging to the phenolic group. The pigments are in glycosylated forms. Anthocyanins responsible for the colors, red, purple, and blue, are in fruits and vegetables. Berries, currants, grapes, and some tropical fruits have high anthocyanins content. Red to purplish blue-colored leafy vegetables, grains, roots, and tubers are the edible vegetables that contain a high level of anthocyanins. Among the anthocyanin pigments, cyanidin-3-glucoside is the major anthocyanin found in most of the plants. The colored anthocyanin pigments have been traditionally used as a natural food colorant. The color and stability of these pigments are influenced by pH, light, temperature, and structure. In acidic condition, anthocyanins appear as red but turn blue when the pH increases. Chromatography has been largely applied in extraction, separation, and quantification of anthocyanins. Besides the use of anthocyanidins and anthocyanins as natural dyes, these colored pigments are potential pharmaceutical ingredients that give various beneficial health effects. Scientific studies, such as cell culture studies, animal models, and human clinical trials, show that anthocyanidins and anthocyanins possess antioxidative and antimicrobial activities, improve visual and neurological health, and protect against various non-communicable diseases. These studies confer the health effects of anthocyanidins and anthocyanins, which are due to their potent antioxidant properties. Different mechanisms and pathways are involved in the protective effects, including free-radical scavenging pathway, cyclooxygenase pathway, mitogen-activated protein kinase pathway, and inflammatory cytokines signaling. Therefore, this review focuses on the role of anthocyanidins and anthocyanins as natural food colorants and their nutraceutical properties for health. Abbreviations: CVD: Cardiovascular disease VEGF: Vascular endothelial growth factor

Identifying Failure-Inducing Combinations in a Combinatorial Test Set

Abstract- A �-way combinatorial test set is designed to detect failures that are triggered by combinations involving no more than � parameters. Assume that we have executed a �-way test set and some tests have failed. A natural question to ask is: what combinations have caused these failures? Identifying such combinations can facilitate the debugging effort, e.g., by reducing the scope of the code that needs to be inspected. In this paper, we present an approach to identifying failureinducing combinations, i.e., combinations that have caused some tests to fail. Given a �-way test set, our approach first identifies and ranks a set of suspicious combinations, which are candidates that are likely to be failure-inducing combinations. Next, it generates a set of new tests, which can be executed to refine the ranking of suspicious combinations in the next iteration. This process can be repeated until a stopping condition is satisfied. We conducted an experiment in which our approach was applied to several benchmark programs. The experimental results show that our approach can effectively and efficiently identify failure-inducing combinations in these programs