Shared and unique common genetic determinants between pediatric and adult celiac disease

Table S1. Functional profiles of the top non-HLA association signals identified in Paediatric CD and Adult CD among north Indians. Table S2. Test of heterogeneity (Breslow-Day test) for associated SNPs in PaediatricCD and AdultCD groups. Table S3. cis-eQTL evaluation of associated SNPs. Table S4. GRAIL analysis revealed seven genes with significant (p <0.05) interaction with 39 known non-HLA coeliac disease loci. These seven genes are from four loci identified in this study. (DOCX 26 kb

Exploring the Scope of Prognosis Agent Technology in Digital Manufacturing

It is an established fact that the last decade is evident for the advancement in manufacturing sector by the use of various digital manufacturing (DM) techniques. Agent technology has contributed far in the DM by simplifying and adding synergy to the various functionaries in form of static and mobile agents. The agents contribute in the paradigms of designing, diagnosis, production, marketing etc. In the international business market, the agent technology has increased the competence by providing fast, error free, customized services. The paper first reviews the work done in the field of applications of agent technology in digital manufacturing including the role of agent technology in prognosis and then the research object is to develop a framework for the prognosis of digital data feeded to the manufacturing facilities of DM system. The paper focus on the introduction and brief description of the manufacturing prognosis agent in context to Digital manufacturing. Key words: manufacturing prognosis agent, digital manufacturing, prognosis, agent technology, digital dat

Loss of genetic diversity and inbreeding in Kashmir red deer (Cervus elaphus hanglu) of Dachigam National Park, Jammu & Kashmir, India

BACKGROUND: Hangul (Cervus elaphus hanglu), the eastern most subspecies of red deer, is now confined only to the mountains in the Kashmir region of Jammu & Kashmir State of India. It is of great conservation significance as this is the last and only hope for Asiatic survivor of the red deer species in India. Wild population of free ranging hangul deer inhabiting in and around Dachigam National Park was genetically assessed in order to account for constitutive genetic attributes of hangul population using microsatellite markers. RESULTS: In a pool of 36 multi-locus genotypes, 30 unique individuals were identified based on six microsatellite loci. The estimated cumulative probability of identity assuming all individuals were siblings (P(ID) sibs) was 0.009 (9 in 1000). Altogether, 49 different alleles were observed with mean (± s.e.) allelic number of 8.17 ± 1.05, ranging from 5 to 11 per locus. The observed heterozygosity ranged between 0.08 and 0.83, with mean 0.40 ± 0.11 and the inbreeding coefficient ranged between −0.04 and 0.87 with mean 0.38 ± 0.15. Majority of loci (5/6) were found to be informative (PIC value > 0.5). All loci deviated from Hardy-Weinberg equilibrium except Ca-38 (P > 0.05) and none of the pairs of loci showed significant linkage disequilibrium except the single pair of Ca-30 and Ca-43 (P < 0.05). CONCLUSIONS: The preliminary findings revealed that hangul population is significantly inbred and exhibited a low genetic diversity in comparison to other deer populations of the world. We suggest prioritizing the potential individuals retaining high heterozygosity for ex situ conservation and genetic monitoring of the hangul population should be initiated covering the entire distribution range to ensure the long term survival of hangul. We speculate further ignoring genetics attributes may lead to a detrimental effect which can negatively influence the reproductive fitness and survivorship of the hangul population in the wild

Survey of Green Corrosion Inhibitor for Metal Surface

Almost every engineering profession and aspect of our everyday lives has suffered harm due to corrosion in recent decades. It is seen as a potentially catastrophic environmental issue that calls for continuous investigation into potential remedies. Green corrosion inhibitors, which are affordable, environmentally friendly, and derived from plant extracts, are the most effective solution to address this problem, according to recent research. The mechanism, categorization, and active functional group of green inhibitors are all explained in detail in this review. Furthermore, a variety of plant extracts that inhibit corrosion are discussed, along with the variables that influence their efficacy

Phase Shift Analysis for Alpha-alpha Elastic Scattering using Phase Function Method for Gaussian Local Potential

The phase shifts for α- α scattering have been modeled using a two parameter Gaussian local potential. The time independent Schrodinger equation (TISE) has been solved iteratively using Monte-Carlo approach till the S and D bound states of the numerical solution match with the experimental binding energy data in a variational sense. The obtained potential with best fit parameters is taken as input for determining the phase-shifts for the S channel using the non-linear first order differential equation of the phase function method (PFM). It is numerically solved using 5th order Runge-Kutta (RK-5) technique. To determine the phase shifts for the ℓ=2 and 4 scattering state i.e. D and G-channel, the inversion potential parameters have been determined using variational Monte-Carlo (VMC) approach to minimize the realtive mean square error w.r.t. the experimental data

Evaluating the effect of dexmedetomidine premedication on the quality of subarachnoid block, haemodynamics and sedation in patients undergoing lower limb surgeries: a prospective randomized controlled trial

Background: Present study was designed to evaluate the effect of intravenous dexmedetomidine on haemodynamics, sedation and quality of spinal anaesthesia with 0.5% hyperbaric bupivacaine.Methods: Sixty ASA grade 1 and 2, 18-60 years aged patients scheduled for elective lower limb surgeries were randomly divided into two groups: Group C (Control) and Group D (Study), received intravenous normal saline 10ml and intravenous dexmedetomidine 1μg/kg in dilution of 10ml respectively over 10minutes duration, 10minutes before subarachnoid block with 2.5ml of 0.5% hyperbaric bupivacaine. The heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), quality of sensory and motor block and level of sedation were monitored intraoperatively and postoperatively.Results: The heart rate was statistically significantly decreased in group D both intraoperatively and postoperatively. Intraoperative and postoperative SBP and DBP were lower in dexmedetomidine group but clinically that was insignificant. Intraoperative Ramsay sedation scores were significantly higher in dexmedetomidine group (3.49±0.240) as compared to control group (2.51±0.249) (p<0.001) but the patients were easily arousable. The duration of sensory blockade (208.83±9.53 min vs 162.83±9.62 min), duration for 2 dermatomal regression of sensory blockade (146.5±10.013min vs 98±8.57min) and the duration for motor block regression to Modified Bromage scale 0 (167.33±10.5min vs 137.83±11.94min) were significantly prolonged in dexmedetomidine group as compared to control group. The highest level of sensory blockade was also significantly higher in dexmedetomidine group (T6.90±0.759 vs T7.60±0.621). There was no difference in the time for attaining highest level of sensory blockade, time taken for motor blockade to reach Modified Bromage Scale 3 between both the groups. Average 24hr mean VAS score was significantly lower in dexmedetomidine group (1.37±0.15 vs1.72±0.17, p<0.001). Time to first request for rescue analgesic was also significantly longer in dexmedetomidine group (mean 174.33min vs 143.5min). Average 24hour consumption of tramadol analgesic was significantly higher in control group as compared to dexmedetomidine group (391.86±111.62mg vs 279.86±80.55mg, p<0.001).Conclusions: Intravenous dexmedetomidine significantly prolongs the duration of sensory and motor block of bupivacaine spinal anaesthesia. It provides excellent sedation and analgesia. Dexmedetomidine induced decrease in heart rate, systolic/diastolic blood pressure are not clinically significant.

Synthesis and in vitro evaluation of novel isatinincorporated thiadiazole hybrids as potential anti-breast cancer agents

Purpose: To synthesis and characterize some novel isatin-incorporated thiadiazoles and screen them for anti-breast cancer activity in human breast adenocarcinoma cells (MCF-7).Method: A series of isatin incorporated Schiff bases of thiadiazoles (3a-3l) was synthesized by reaction of substituted thiadiazoles (1a-1d) with isatin (2a) and N-alkyl substituted isatin (2b-2c) and characterized by elemental analysis, IR, 1H NMR, 13C NMR and LCMS. The newly synthesized compounds were screened for their in-vitro cytotoxicity against MCF-7 cell lines by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric and Sulforhodamine B (SRB) methods.Results: Compounds 3a, 3c, 3d, 3g and 3j showed anticancer activity in both MTT and SRB assay. Compound 3-(5-(4-chlorophenyl)-1,3,4-thiadiazol-2-ylimino)-1-ethylindolin-2-one (3g) showed most potent cytotoxic activity against MCF-7 cell lines.Conclusion: The novel isatin incorporated thiadiazoles synthesized and characterized in this study possess anti-cancer activities in human breast adenocarcinoma cells (MCF-7). This can possibly lead to emergence of new anti-breast cancer agents.Keywords: Thiadiazoles, Isatin, In-vitro cytotoxicity, Human breast adenocarcinoma cells (MCF-7), SRB assa

Land Use/Land Cover Change and Environmental Impact Analysis of Ramgarh-Naudiha Region in Uttar Pradesh, India through Geospatial Technology

Rapidly changing LULC scenario with growing population is of great concern in the modern world. The present study was undertaken to evaluate the changes in LULC pattern in Ramgarh-Naudiha region of Sonbhadra district, UP, over 20 years during 1998-2018 using datasets from the Landsat Thematic Mapper (TM) 5 and Landsat 8 (OLI/TIRS) satellites. LULC map for the chosen period has been generated by unsupervised classification with maximum likelihood algorithm. Results indicate that the study area is vulnerable to such LULC changes due to its sensitive geographic location. It is found that the major changes did happen in agriculture, forest, wasteland and water bodies. Agriculture and Forest areas have decreased by similar to 2 and 6.56% respectively in the study period. The wastelands had increased fast from 5.08% in 1998 to 18.87% in 2018 at the cost of the forest cover and agricultural land respectively. In 1998, water bodies were 7.49%, whereas, it has decreased to 2.04% in 2018. On the contrary, urban fringe area has grown from 0.33% in 1998 to 0.49% in 2018 especially due to population growth. The present study concludes that this LULC analysis will increase awareness and help in taking necessary action in appropriate land use planning and management

1′-Ethyl­sulfanyl-1,1′-bicyclo­hexyl-2-one

There are two independent molecules in the asymmetric unit of the title cyclo­hexa­none derivative, C14H24OS, in which both cyclo­hexane rings exhibit chair conformations. They are also equatorial to each other, which permits the ethanethiol substituent to be in a syn conformation with the α-H atom of the parent attached cyclo­hexa­none