In vitro evaluation of topical gel prepared using natural polymer

Nimesulide is a second generation non–steroidal anti–inflammatory agent, which is widely used in the long term therapy of rheumatoid arthritis, in alleviating pain and inflammation. But its short half-life (only 3–4 hr), so its causes more fluctuation. After oral administration Nimesulide causes to produces heart burn, nausea, loose motions, pruritus, etc. The present study based on the preparation of bioadhesive topical gel of Nimesulide, so as to avoid all gastric side effects. For the preparation of bioadhesive topical gel natural polymer aegel marmelos (plant Bale) was used. Bioadhesive polymers are the agents which increases the contact between the formulation and biological membrane, so as to avoid the fluctuation of formulation and behave as a sustained release formulation. In the present study, prepared bioadhesive topical gel was evaluated with the help of different parameters like drug content, spreadability, extrudability, swelling index study, in–vitro drug diffusion study, in-vitro drug release kinetic study and ex–vivo bioadhesive measurement. On the basis of in–vitro drug diffusion study and ex–vivo bioadhesive measurement property of gel, we have concluded that natural polymer aegel marmelos is the best polymer for the preparation of sustained release bioadhesive topical gel.Keywords: Topical gel; Bioadhesion; Natural polyme

Modern Biomedical Technologies versus Emerging Pathogenic Microbes: Insights into the Confronting Situation

The interaction between modern biomedical technologies and newly identified pathogenic microbes has become a crucial focus in the field of healthcare and illness management. The ongoing progress in technology is transforming the field of medical science. However, the advent of new and changing microbial dangers presents obstacles that require creative solutions. This article explores the delicate equilibrium between the capabilities of advanced biomedical technologies and the continuous development of disease-causing microorganisms. It provides new insights that influence the ongoing struggle between scientific advancement and the problems posed by these microbes

In vitro evaluation of topical gel prepared using natural polymer

Nimesulide is a second generation non–steroidal anti–inflammatory agent, which is widely used in the long term therapy of rheumatoid arthritis, in alleviating pain and inflammation. But its short half-life (only 3–4 hr), so its causes more fluctuation. After oral administration Nimesulide causes to produces heart burn, nausea, loose motions, pruritus, etc. The present study based on the preparation of bioadhesive topical gel of Nimesulide, so as to avoid all gastric side effects. For the preparation of bioadhesive topical gel natural polymer aegel marmelos (plant Bale) was used. Bioadhesive polymers are the agents which increases the contact between the formulation and biological membrane, so as to avoid the fluctuation of formulation and behave as a sustained release formulation. In the present study, prepared bioadhesive topical gel was evaluated with the help of different parameters like drug content, spreadability, extrudability, swelling index study, in–vitro drug diffusion study, in-vitro drug release kinetic study and ex–vivo bioadhesive measurement. On the basis of in–vitro drug diffusion study and ex–vivo bioadhesive measurement property of gel, we have concluded that natural polymer aegel marmelos is the best polymer for the preparation of sustained release bioadhesive topical gel.Keywords: Topical gel; Bioadhesion; Natural polyme

Sums of matrix-valued wave packet frames in (L^2(ℝ^d,ℂ^{stimes r}))

The purpose of this paper is to first show relations between wave packet frame bounds and the scalars associated with finite sum of matrix-valued wave packet frames for the matrix-valued function space (L^2(ℝ^d, ℂ^{stimes r}). A sufficient condition with explicit wave packet frame bounds for finite sum of matrix-valued wave packet frames in terms of scalars and frame bounds associated with the finite sum of frames is given. An optimal estimate of wave packet frame bounds for the finite sum of matrix-valued wave packet frames is presented. In the second part, we show that the rate of convergence of the frame algorithm can be increased by using frame bounds and scalars associated with the finite sum of frames. Finally, a necessary and sufficient condition for finite sum of matrix-valued wave packet frames in terms of series associated with wave packet vectors is given

AffyMAPSDetector: a software tool to characterize Affymetrix GeneChip™ expression arrays with respect to SNPs

<p>Abstract</p> <p>Background</p> <p>Affymetrix gene expression arrays incorporate paired perfect match (PM) and mismatch (MM) probes to distinguish true signals from those arising from cross-hybridization events. A MM signal often shows greater intensity than a PM signal; we propose that one underlying cause is the presence of allelic variants arising from single nucleotide polymorphisms (SNPs). To annotate and characterize SNP contributions to anomalous probe binding behavior we have developed a software tool called AffyMAPSDetector.</p> <p>Results</p> <p>AffyMAPSDetector can be used to describe any Affymetrix expression GeneChip™ with respect to SNPs. When AffyMAPSDetector was run on GeneChip™ HG-U95Av2 against dbSNP-build-123, we found 7286 probes (belonging to 2,582 probesets) containing SNPs, out of which 325 probes contained at least one SNP at position 13. Against dbSNP-build-126, 8758 probes (belonging to 3,002 probesets) contained SNPs, of which 409 probes contained at least one SNP at position 13. Therefore, depending on the expressed allele, the MM probe can sometimes be the transcript complement. This information was used to characterize probe measurements reported in a published, well-replicated lung adenocarcinoma study. The total intensity distributions showed that the SNP-containing probes had a larger negative mean intensity difference (PM-MM) and greater range of the difference than did probes without SNPs. In the sample replicates, SNP-containing probes with reproducible intensity ratios were identified, allowing selection of SNP probesets that yielded unique sample signatures. At the gene expression level, use of the (MM-PM) value for SNP-containing probes resulted in different Presence/Absence calls for some genes. Such a change in status of the genes has the clear potential for influencing downstream clustering and classification results.</p> <p>Conclusion</p> <p>Output from this tool characterizes SNP-containing probes on GeneChip™ microarrays, thus improving our understanding of factors contributing to expression measurements. The pattern of SNP binding examined so far indicates distinct behavior of the SNP-containing probes and has the potential to help us identify new SNPs. Knowing which probes contain SNPs provides flexibility in determining whether to include or exclude them from gene-expression intensity calculations; selected sets of SNP-containing probes produce sample-unique signatures.</p> <p>AffyMAPSDetector information is available at <url>http://www.binf.gmu.edu/weller/BMC_bioinformatics/AffyMapsDetector/index.html</url></p