Avaliação da pressão arterial sistêmica em quinhentas crianças no ambulatório de pediatria geral do inamps - Criciúma - SC.

Trabalho de Conclusão de Curso - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Departamento de Pediatria, Curso de Medicina, Florianópolis, 198

Analysis of Diffusion of Ras2 in Saccharomyces cerevisiae Using Fluorescence Recovery after Photobleaching

Binding, lateral diffusion and exchange are fundamental dynamic processes involved in protein association with cellular membranes. In this study, we developed numerical simulations of lateral diffusion and exchange of fluorophores in membranes with arbitrary bleach geometry and exchange of the membrane localized fluorophore with the cytosol during Fluorescence Recovery after Photobleaching (FRAP) experiments. The model simulations were used to design FRAP experiments with varying bleach region sizes on plasma-membrane localized wild type GFP-Ras2 with a dual lipid anchor and mutant GFP-Ras2C318S with a single lipid anchor in live yeast cells to investigate diffusional mobility and the presence of any exchange processes operating in the time scale of our experiments. Model parameters estimated using data from FRAP experiments with a 1 micron x 1 micron bleach region-of-interest (ROI) and a 0.5 micron x 0.5 micron bleach ROI showed that GFP-Ras2, single or dual lipid modified, diffuses as single species with no evidence of exchange with a cytoplasmic pool. This is the first report of Ras2 mobility in yeast plasma membrane. The methods developed in this study are generally applicable for studying diffusion and exchange of membrane associated fluorophores using FRAP on commercial confocal laser scanning microscopes.Comment: Accepted for publication in Physical Biology (2010). 28 pages, 7 figures, 3 table

Hydrology, Water Chemistry, and Vegetation Characteristics of a Tamarack Bog in Bath Township, Ohio: Towards Restoration and Enhancement

Author Institution: Dept. of Biology, University of Akron, OHAuthor Institution: Dept. of Botany, University of British Columbia, Vancouver, BC, CanadaAuthor Institution: Dept. of Natural Resource Science, Thompson Rivers University, Kamloops, BC, CanadaThe current state of the Bath Tamarack Bog has raised concern about the health and function of the system. Only 6 tamarack (Larix laricina) trees remain, while deciduous trees, particularly red maple (Acer rubrum) and invasive species such as glossy buckthorn (Rhamnus frangula) and multiflora rose (Rosa multiflora), dominate the bog. Our purpose was to assess the physical, chemical, and biological properties of the tamarack bog. Environmental and biological properties of Bath Tamarack Bog were measured from May 2001 through November 2002. In 2001, the center of the bog experienced water levels below those typically found in bogs, yet experienced normal water levels in the following year. Water chemistry results indicate the pH is much greater than that characteristic of a typical bog, ranging from 5.94 to 7.41. Nutrient levels fluctuated and were generally higher for calcium, potassium, and phosphate than a typical bog, while nitrogen levels remained low. These results indicate that the bog is not functioning normally and is in decline. The degradation of the bog is most likely due to anthropogenic activity. Ditching occurred between 1963 and 1969 and seems to have induced the progression of red maple trees and invasive species into the bog by lowering water levels. Since 1938, the first aerial photo we have record of, the bog has reduced to approximately a third of its size, which is approximately 1.99 hectares. The bog appears to be in a late successional stage, rapidly changing to a forested wetland. We discuss possible management and restoration efforts needed to restore or enhance the tamarack bog, including 1) planting Sphagnum mats, 2) introducing tamarack seedlings, 3) controlling invasive species, and 4) maintaining the hydrology close to the soil surface. All of these measures are suggested in association with educational outreach

Free Speech or Trademark Protections: Do Advocacy Groups and Government Agencies Deserve Extra Protection

Since October 2009, the American judicial system has been posed with yet another lawsuit in the oft recurring battle between trademark protections and right to freedom of expression, specifically the right to parody. The Yes Men, a parody troop, in a stunt which confused numerous news outlets, held a press conference as members of the United States Chamber of Commerce. The Chamber responded by suing the Yes Men for, amongst other causes of action, trademark infringement pursuant to the Lanham Act. This note will first analyze the history of the debate between the conflicting right of free expression and consumer protection statutes, culminating in the author\u27s proposal that government agencies and lobbyist groups such as the Chamber deserve more protection than other entitites due to their level of economic and political importance. This note recognizes the importance of First Amendment rights in the political landscape, but also recognizes the practical necessity of formulating distinctions between various types and potential effects of parody

CREB is a critical regulator of normal hematopoiesis and leukemogenesis

The cAMP-responsive element binding protein (CREB) is a 43-kDa nuclear transcription factor that regulates cell growth, memory, and glucose homeostasis. We showed previously that CREB is amplified in myeloid leukemia blasts and expressed at higher levels in leukemia stem cells from patients with myeloid leukemia. CREB transgenic mice develop myeloproliferative disease after 1 year, but not leukemia, suggesting that CREB contributes to but is not sufficient for leukemogenesis. Here, we show that CREB is most highly expressed in lineage negative hematopoietic stem cells (HSCs). To understand the role of CREB in hematopoietic progenitors and leukemia cells, we examined the effects of RNA interference (RNAi) to knock down CREB expression in vitro and in vivo. Transduction of primary HSCs or myeloid leukemia cells with lentiviral CREB shRNAs resulted in decreased proliferation of stem cells, cell- cycle abnormalities, and inhibition of CREB transcription. Mice that received transplants of bone marrow transduced with CREB shRNA had decreased committed progenitors compared with control mice. Mice injected with Ba/F3 cells expressing either Bcr-Abl wild-type or T315I mutation with CREB shRNA had delayed leukemic infiltration by bioluminescence imaging and prolonged median survival. Our results suggest that CREB is critical for normal myelopoiesis and leukemia cell proliferation

The diet-body offset in human nitrogen isotopic values: a controlled dietary study

The ‘trophic level enrichment’ between diet and body results in an overall increase in nitrogen isotopic values as the food chain is ascended. Quantifying the diet–body Δ15N spacing has proved difficult, particularly for humans. The value is usually assumed to be +3-5‰ in the archaeological literature. We report here the first (to our knowledge) data from humans on isotopically known diets, comparing dietary intake and a body tissue sample, that of red blood cells. Samples were taken from 11 subjects on controlled diets for a 30-d period, where the controlled diets were designed to match each individual’s habitual diet, thus reducing problems with short-term changes in diet causing isotopic changes in the body pool. The Δ15Ndiet-RBC was measured as +3.5‰. Using measured offsets from other studies, we estimate the human Δ15Ndiet-keratin as +5.0-5.3‰, which is in good agreement with values derived from the two other studies using individual diet records. We also estimate a value for Δ15Ndiet-collagen of ≈6‰, again in combination with measured offsets from other studies. This value is larger than usually assumed in palaeodietary studies, which suggests that the proportion of animal protein in prehistoric human diet may have often been overestimated in isotopic studies of palaeodiet

A Passport to Successful Transfer Appreciative Advising

How do we appreciate? The Appreciative Advising framework facilitates and fosters optimal student development and success.  But, how may this be conducted in a community college setting when academic advising sessions and resources have competing priorities? Learn from the innovative practice and partnership between Ivy Tech Community College, IUPUI’s Department of Psychology, and the IUPUI-Ivy Tech Coordinated Programs Passport Office to enable the necessary foundation for Appreciative Advising sessions at your community college.  Our practice encompasses workshops and presentations for psychology students at Ivy Tech which includes a transferable statewide competency-based curriculum.  These initiatives enable a follow up one-on-one advising session to explore ‘outside of the classroom’ needs.  We appreciate our students in innovative ways by building relationships to ensure that they are being asked the right questions to achieve their goals and overcome challenges.