Group delay in THz spectroscopy with ultra-wideband log-spiral antennae

We report on the group delay observed in continuous-wave terahertz spectroscopy based on photomixing with phase-sensitive homodyne detection. We discuss the different contributions of the experimental setup to the phase difference \Delta\phi(\nu) between transmitter arm and receiver arm. A simple model based on three contributions yields a quantitative description of the overall behavior of \Delta\phi(\nu). Firstly, the optical path-length difference gives rise to a term linear in frequency. Secondly, the ultra-wideband log-spiral antennae effectively radiate and receive in a frequency-dependent active region, which in the most simple model is an annular area with a circumference equal to the wavelength. The corresponding term changes by roughly 6 pi between 100 GHz and 1 THz. The third contribution stems from the photomixer impedance. In contrast, the derivative (d\Delta\phi / d\nu) is dominated by the contribution of periodic modulations of \Delta\phi(\nu) caused by standing waves, e.g., in the photomixers' Si lenses. Furthermore, we discuss the Fourier-transformed spectra, which are equivalent to the waveform in a time-domain experiment. In the time domain, the group delay introduced by the log-spiral antennae gives rise to strongly chirped signals, in which low frequencies are delayed. Correcting for the contributions of antennae and photomixers yields sharp peaks or "pulses" and thus facilitates a time-domain-like analysis of our continuous-wave data.Comment: 7 pages, 7 figure

Self-normalizing phase measurement in multimode terahertz spectroscopy based on photomixing of three lasers

Photomixing of two near-infrared lasers is well established for continuous-wave terahertz spectroscopy. Photomixing of three lasers allows us to measure at three terahertz frequencies simultaneously. Similar to Fourier spectroscopy, the spectral information is contained in an nterferogram, which is equivalent to the waveform in time-domain spectroscopy. We use one fixed terahertz frequency \nu_ref to monitor temporal drifts of the setup, i.e., of the optical path-length difference. The other two frequencies are scanned for broadband high-resolution spectroscopy. The frequency dependence of the phase is obtained with high accuracy by normalizing it to the data obtained at \nu_ref, which eliminates drifts of the optical path-length difference. We achieve an accuracy of about 1-2 microns or 10^{-8} of the optical path length. This method is particularly suitable for applications in nonideal environmental conditions outside of an air-conditioned laboratory.Comment: 5 pages, 5 figure

Enhancing the stability of a continuous-wave terahertz system by photocurrent normalization

In a continuous-wave terahertz system based on photomixing, the measured amplitude of the terahertz signal shows an uncertainty due to drifts of the responsivities of the photomixers and of the optical power illuminating the photomixers. We report on a simple method to substantially reduce this uncertainty. By normalizing the amplitude to the DC photocurrents in both the transmitter and receiver photomixers, we achieve a significant increase of the stability. If, e.g., the optical power of one laser is reduced by 10%, the normalized signal is expected to change by only 0.3%, i.e., less than the typical uncertainty due to short-term fluctuations. This stabilization can be particularly valuable for terahertz applications in non-ideal environmental conditions outside of a temperature-stabilized laboratory.Comment: 6 figure

Local seismic cultures: the use of timber frame structures in the south of Portugal

Given the fact that using timber frame structures has proven to improve the seismic behavior of vernacular architecture, as has been reported in past earthquakes in many countries, its preservation as a traditional earthquake resistant practice is important. This paper firstly intends to evaluate whether the use of timber frames as a traditional seismic resistant technique for vernacular architecture in the South of Portugal, traditionally a seismic region, is still active. Secondly, the city of Vila Real de Santo António was selected as a case study because it also followed a Pombaline development contemporary to the reconstruction of Lisbon. The plan included the provision of timber frame partition walls for some of the buildings and, thus, an overview of the type of constructions originally conceived is provided. Finally, the alterations done in the original constructions and the current state of the city center are described and the effect of these changes on the seismic vulnerability of the city is discussed.The authors wish to express their gratitude to the Portuguese Science and Technology Foundation (FCT) for the scholarship granted in the scope of the research project 'SEISMIC-V-Vernacular Seismic Culture in Portugal' (PTDC/ATP-AQI/3934/2012)

Neonatal pneumococcal colonisation caused by Influenza A infection alters lung function in adult mice

There is emerging epidemiological data to suggest that upper respiratory tract bacterial colonisation in infancy may increase the risk of developing respiratory dysfunction later in life, and respiratory viruses are known to precipitate persistent colonisation. This study utilized a neonatal mouse model of Streptococcus pneumonia (SP) and influenza A virus (IAV) co-infection, where bronchoalveolar leukocyte infiltration had resolved by adulthood. Only co-infection resulted in persistent nasopharyngeal colonisation over 40 days and a significant increase in airway resistance in response to in vivo methacholine challenge. A significant increase in hysteresivity was also observed in IAV and co-infected mice, consistent with ventilatory heterogeneity and structural changes in the adult lung. Airway hyper-responsiveness was not associated with a detectable increase in goblet cell transdifferentiation, peribronchial smooth muscle bulk or collagen deposition in regions surrounding the airways. Increased reactivity was not observed in precision cut lung slices challenged with methacholine in vitro. Histologically, the airway epithelium appeared normal and expression of epithelial integrity markers (ZO-1, occludin-1 and E-cadherin) were not altered. In summary, neonatal co-infection led to persistent nasopharyngeal colonisation and increased airway responsiveness that was not associated with detectable smooth muscle or mucosal epithelial abnormalities, however increased hysteresivity may reflect ventilation heterogeneity

Seismic behaviour of traditional timber frame walls: experimental results on unreinforced walls

Timber frame buildings are well known as an efficient seismic resistant structure and they are used worldwide. Moreover, they have been specifically adopted in codes and regulations during the XVIII and XIX centuries in the Mediterranean area. These structures generally consist of exterior masonry walls with timber elements embedded which tie the walls together and internal walls which have a timber frame with masonry infill and act as shearwalls. In order to preserve these structureswhich characterizemany cities in theworld it is important to better understand their behaviour under seismic actions. Furthermore, historic technologies could be used even in modern constructions to build seismic resistant buildings using more natural materials with lesser costs. Generally, different types of infill could be applied to timber frame walls depending on the country, among which brick masonry, rubble masonry, hay and mud. The focus of this paper is to study the seismic behaviour of the walls considering different types of infill, specifically: masonry infill, lath and plaster and timber frame with no infill. Static cyclic tests have been performed on unreinforced timber frame walls in order to study their seismic capacity in terms of strength, stiffness, ductility and energy dissipation. The tests showed how in the unreinforced condition, the infill is able to guarantee a greater stiffness, ductility and ultimate capacity of the wall.The authors would like to acknowledge Eng. Filipe Ferreira and A.O.F. (Augusto Oliveira Ferreira & C Lda.) for their expertise and collaboration in the construction of the wall specimens. The first author would also like to acknowledge the Portuguese Science and Technology Foundation (FCT) for its financial support through grant SFRH / BD / 61908 / 2009

Year-round stratospheric aerosol backscatter ratios calculated from lidar measurements above northern Norway

We present a new method for calculating backscatter ratios of the stratospheric sulfate aerosol (SSA) layer from daytime and nighttime lidar measurements. Using this new method we show a first year-round dataset of stratospheric aerosol backscatter ratios at high latitudes. The SSA layer is located at altitudes between the tropopause and about 30 km. It is of fundamental importance for the radiative balance of the atmosphere. We use a state-of-the-art Rayleigh-Mie-Raman lidar at the Arctic Lidar Observatory for Middle Atmosphere Research (ALOMAR) station located in northern Norway (69N, 16E; 380ma.s.l.). For nighttime measurements the aerosol backscatter ratios are derived using elastic and inelastic backscatter of the emitted laser wavelengths 355, 532 and 1064nm. The setup of the lidar allows measurements with a resolution of about 5 min in time and 150 m in altitude to be performed in high quality, which enables the identification of multiple sub-layers in the stratospheric aerosol layer of less than 1 km vertical thickness. We introduce a method to extend the dataset throughout the summer when measurements need to be performed under permanent daytime conditions. For that purpose we approximate the backscatter ratios from color ratios of elastic scattering and apply a correction function. We calculate the correction function using the average backscatter ratio profile at 355nm from about 1700 h of nighttime measurements from the years 2000 to 2018. Using the new method we finally present a year-round dataset based on about 4100 h of measurements during the years 2014 to 2017. © Author(s) 2019

Dystroglycan versatility in cell adhesion: a tale of multiple motifs

Dystroglycan is a ubiquitously expressed heterodimeric adhesion receptor. The extracellular a-subunit makes connections with a number of laminin G domain ligands including laminins, agrin and perlecan in the extracellular matrix and the transmembrane b-subunit makes connections to the actin filament network via cytoskeletal linkers including dystrophin, utrophin, ezrin and plectin, depending on context. Originally discovered as part of the dystrophin glycoprotein complex of skeletal muscle, dystroglycan is an important adhesion molecule and signalling scaffold in a multitude of cell types and tissues and is involved in several diseases. Dystroglycan has emerged as a multifunctional adhesion platform with many interacting partners associating with its short unstructured cytoplasmic domain. Two particular hotspots are the cytoplasmic juxtamembrane region and at the very carboxy terminus of dystroglycan. Regions which between them have several overlapping functions: in the juxtamembrane region; a nuclear localisation signal, ezrin/radixin/moesin protein, rapsyn and ERK MAP Kinase binding function, and at the C terminus a regulatory tyrosine governing WW, SH2 and SH3 domain interactions. We will discuss the binding partners for these motifs and how their interactions and regulation can modulate the involvement of dystroglycan in a range of different adhesion structures and functions depending on context. Thus dystroglycan presents as a multifunctional scaffold involved in adhesion and adhesion-mediated signalling with its functions under exquisite spatiotemporal regulation

Prostaglandin signalling regulates ciliogenesis by modulating intraflagellar transport

Cilia are microtubule-based organelles that mediate signal transduction in a variety of tissues. Despite their importance, the signalling cascades that regulate cilium formation remain incompletely understood. Here we report that prostaglandin signalling affects ciliogenesis by regulating anterograde intraflagellar transport (IFT). Zebrafish leakytail (lkt) mutants show ciliogenesis defects, and the lkt locus encodes an ATP-binding cassette transporter (ABCC4). We show that Lkt/ABCC4 localizes to the cell membrane and exports prostaglandin E2 (PGE2), a function that is abrogated by the Lkt/ABCC4T804M mutant. PGE2 synthesis enzyme cyclooxygenase-1 and its receptor, EP4, which localizes to the cilium and activates the cyclic-AMP-mediated signalling cascade, are required for cilium formation and elongation. Importantly, PGE2 signalling increases anterograde but not retrograde velocity of IFT and promotes ciliogenesis in mammalian cells. These findings lead us to propose that Lkt/ABCC4-mediated PGE2 signalling acts through a ciliary G-protein-coupled receptor, EP4, to upregulate cAMP synthesis and increase anterograde IFT, thereby promoting ciliogenesis