Two phase galaxy formation: The Evolutionary Properties of Galaxies

We use our model for the formation and evolution of galaxies within a two-phase galaxy formation scenario, showing that the high-redshift domain typically supports the growth of spheroidal systems, whereas at low redshifts the predominant baryonic growth mechanism is quiescent and may therefore support the growth of a disc structure. Under this framework we investigate the evolving galaxy population by comparing key observations at both low and high-redshifts, finding generally good agreement. By analysing the evolutionary properties of this model, we are able to recreate several features of the evolving galaxy population with redshift, naturally reproducing number counts of massive star-forming galaxies at high redshifts, along with the galaxy scaling relations, star formation rate density and evolution of the stellar mass function. Building upon these encouraging agreements, we make model predictions that can be tested by future observations. In particular, we present the expected evolution to z=2 of the super-massive black hole mass function, and we show that the gas fraction in galaxies should decrease with increasing redshift in a mass, with more and more evolution going to higher and higher masses. Also, the characteristic transition mass from disc to bulge dominated system should decrease with increasing redshift.Comment: 15 pages, 11 figures. Version polished for publication in MNRA

Rat pial microvascular responses to melatonin during bilateral common carotid artery occlusion and reperfusion

The present study assessed the in vivo rat pial microvascular responses induced by melatonin during brain hypoperfusion and reperfusion (RE) injury. Pial microcirculation of male Wistar rats was visualized by fluorescence microscopy through a closed cranial window. Hypoperfusion was induced by bilateral common carotid artery occlusion (BCCAO, 30 min); thereafter, pial microcirculation was observed for 60 min. Arteriolar diameter, permeability increase, leukocyte adhesion to venular walls, perfused capillary length (PCL), and capillary red blood cell velocity (V(RBC) ) were investigated by computerized methods. Melatonin (0.5, 1, 2 mg/kg b.w.) was intravenously administered 10 min before BCCAO and at the beginning of RE. Pial arterioles were classified in five orders according to diameter, length, and branchings. In control group, BCCAO caused decrease in order 2 arteriole diameter (by 17.5 ± 3.0% of baseline) that was reduced by 11.8 ± 1.2% of baseline at the end of RE, accompanied by marked leakage and leukocyte adhesion. PCL and capillary V(RBC) decreased. At the end of BCCAO, melatonin highest dosage caused order 2 arteriole diameter reduction by 4.6 ± 2.0% of baseline. At RE, melatonin at the lower dosages caused different arteriolar responses. The highest dosage caused dilation in order 2 arteriole by 8.0 ± 1.5% of baseline, preventing leakage and leukocyte adhesion, while PCL and V(RBC) increased. Luzindole (4 mg/kg b.w.) prior to melatonin caused order 2 arteriole constriction by 12.0 ± 1.5% of baseline at RE, while leakage, leukocyte adhesion, PCL and V(RBC) were not affected. Prazosin (1 mg/kg b.w.) prior to melatonin did not significantly change melatonin's effects. In conclusion, melatonin caused different responses during hypoperfusion and RE, modulating pial arteriolar tone likely by MT1 and MT2 melatonin receptors while preventing blood-brain barrier changes through its free radical scavenging action

Cyclotron production of high–specific activity 55Co and in vivo evaluation of the stability of 55Co metal-chelate-peptide complexes

This work describes the production of high–specific activity 55 Co and the evaluation of the stability of 55 Co-metal-chelate-peptide complexes in vivo. 55 Co was produced via the 58 Ni(p,α) 55 Co reaction and purified using anion exchange chromatography with an average recovery of 92% and an average specific activity of 1.96 GBq/μmol. 55 Co-DO3A and 55 Co-NO2A peptide complexes were radiolabeled at 3.7 MBq/μg and injected into HCT-116 tumor xenografted mice. Positron emission tomography (PET) and biodistribution studies were performed at 24 and 48 hours postinjection and compared to those of 55 CoCl 2 . Both 55 Co-metal-chelate complexes demonstrated good in vivo stability by reducing the radiotracers’ uptake in the liver by sixfold at 24 hours with ˜ 1% ID/g and at 48 hours with ˜ 0.5% ID/g and reducing uptake in the heart by fourfold at 24 hours with ˜ 0.7% ID/g and sevenfold at 48 hours with ˜ 0.35% ID/g. These results support the use of 55 Co as a promising new radiotracer for PET imaging of cancer and other diseases

Immuno-PET of epithelial ovarian cancer: Harnessing the potential of CA125 for non-invasive imaging

BACKGROUND: Epithelial ovarian cancer (EOC) is characterized by the overexpression of cancer antigen 125 (CA125), a mucinous glycoprotein that serves as a tumor biomarker. Early diagnosis of EOC is plagued by its asymptomatic nature of progression and the limitations of currently used immunoassay techniques that detect CA125 as a shed antigen in serum samples. Presently, there is no technique available for the in vivo evaluation of CA125 expression in malignant tissues. Moreover, there could be an unexplored pathophysiological time window for the detection of CA125 in EOC, during which it is expressed on tumor cells prior to being shed into the bloodstream. A method for the in vivo evaluation of CA125 expression on ovarian neoplasms earlier along disease progression and/or recurrence can potentially contribute to better disease management. To this end, the present work utilizes an anti-CA125 monoclonal antibody (MAb) and a single-chain variable fragment (scFv) labeled with the positron-emitting radionuclide (64)Cu for preclinical molecular imaging of CA125 expression in vivo. METHODS: Anti-CA125 MAb and scFv were prepared and functionally characterized for target binding prior to being tested as radiotracers in a preclinical setting. RESULTS: Immunoblotting, immunofluorescence, and flow cytometry revealed specific binding of CA125-targeting vectors to NIH:OVCAR-3 cells and no binding to antigen-negative SKOV3 cells. (64)Cu-labeled anti-CA125 MAb and scFv were obtained in specific activities of 296 and 122 MBq/mg, respectively. Both radioimmunoconjugate vectors demonstrated highly selective binding to NIH:OVCAR-3 cells and virtually no binding to SKOV3 cells. In vivo radiopharmacological evaluation using xenograft mouse models injected with (64)Cu-labeled anti-CA125 MAb provided a standardized uptake value (SUV) of 5.76 (29.70 %ID/g) in OVCAR3 tumors 24 h post-injection (p.i.) versus 1.80 (5.91 %ID/g) in SKOV3 tumors. (64)Cu-labeled anti-CA125 scFv provided an SUV of 0.64 (3.21 %ID/g) in OVCAR3 tumors 24 h p.i. versus 0.25 (1.49 %ID/g) in SKOV3 tumors. Results from small-animal PET imaging were confirmed by ex vivo autoradiography and immunohistochemistry. CONCLUSIONS: Radiolabeling of anti-CA125 MAb and scFv with (64)Cu did not compromise their immunoreactivity. Both radioimmunoconjugates presented specific tumor uptake and expected biological clearance profiles. This renders them as potential immuno-PET probes for targeted in vivo molecular imaging of CA125 in EOC. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13550-014-0060-4) contains supplementary material, which is available to authorized users

Feasibility of isotope harvesting at a projectile fragmentation facility: ⁶⁷Cu

The work presented here describes a proof-of-principle experiment for the chemical extraction of (67)Cu from an aqueous beam stop at the National Superconducting Cyclotron Laboratory (NSCL). A 76 MeV/A (67)Cu beam was stopped in water, successfully isolated from the aqueous solution through a series of chemical separations involving a chelating disk and anion exchange chromatography, then bound to NOTA-conjugated Herceptin antibodies, and the bound activity was validated using instant thin-layer chromatography (ITLC). The chemical extraction efficiency was found to be 88 ± 3% and the radiochemical yield was ≥95%. These results show that extraction of radioisotopes from an aqueous projectile-fragment beam dump is a feasible method for obtaining radiochemically pure isotopes

Evidence of strong quasar feedback in the early Universe

Most theoretical models invoke quasar driven outflows to quench star formation in massive galaxies, this feedback mechanism is required to account for the population of old and passive galaxies observed in the local universe. The discovery of massive, old and passive galaxies at z=2, implies that such quasar feedback onto the host galaxy must have been at work very early on, close to the reionization epoch. We have observed the [CII]158um transition in SDSSJ114816.64+525150.3 that, at z=6.4189, is one of the most distant quasars known. We detect broad wings of the line tracing a quasar-driven massive outflow. This is the most distant massive outflow ever detected and is likely tracing the long sought quasar feedback, already at work in the early Universe. The outflow is marginally resolved on scales of about 16 kpc, implying that the outflow can really affect the whole galaxy, as required by quasar feedback models. The inferred outflow rate, dM/dt > 3500 Msun/yr, is the highest ever found. At this rate the outflow can clean the gas in the host galaxy, and therefore quench star formation, in a few million years.Comment: 5 pages, 3 figures, accepted for publication in MNRAS Letter

The Herschel Stripe 82 Survey (HerS): maps and early catalog

We present the first set of maps and band-merged catalog from the Herschel Stripe 82 Survey (HerS). Observations at 250, 350, and 500μm were taken with the Spectral and Photometric Imaging Receiver instrument aboard the Herschel Space Observatory. HerS covers 79deg 2 along the SDSS Stripe 82 to an average depth of 13.0, 12.9, and 14.8mJybeam −1 (including confusion) at 250, 350, and 500μm, respectively. HerS was designed to measure correlations with external tracers of the dark matter density field—either point-like (i.e., galaxies selected from radio to X-ray) or extended (i.e., clusters and gravitational lensing)—in order to measure the bias and redshift distribution of intensities of infrared-emitting dusty star-forming galaxies and active galactic nuclei. By locating HerS in Stripe 82, we maximize the overlap with available and upcoming cosmological surveys. The band-merged catalog contains 3.3 × 10 4 sources detected at a significance of ?3σ (including confusion noise). The maps and catalog are available at http://www.astro.caltech.edu/hers/