Phosphoenolpyruvate carboxykinase, pyruvate orthophosphate dikinase and isocitrate lyase in both tomato fruits and leaves, and in the flesh of peach and some other fruits

In this study the occurrence of a number of enzymes involved in gluconeogenesis was investigated in both tomato fruits and leaves during their development and senescence and in some other fruits. The enzymes studied were phosphoenolpyruvate carboxykinase (PEPCK), pyruvate orthophosphate dikinase (PPDK) and glyoxysomal isocitrate lyase (ICL). PPDK was detected in the ripe flesh of tomato, and much smaller amounts were detected in the flesh of both peach and pepper, whereas it was not detected (not present or at very low abundance) in the other fruits which were investigated (apricot, aubergine, blackberry, blueberry, cherry, grape, plum, raspberry and red current). By contrast PEPCK was present in the flesh of all the fruits investigated. Very small amounts of ICL were detected in ripe tomato flesh. PEPCK was present in the skin, flesh, locular gel and columella of tomato fruit, and in these its abundance increased greatly during ripening. PPDK showed a similar distribution, however, its abundance did not increase during ripening. PEPCK was not detected in tomato leaves at any stage of their development or senescence. The content of PPDK g−1 fresh weight (FW) increased in tomato leaves as they matured, however, it declined during their senescence. In tomato leaves the content of ICL g−1 FW increased until the mid-stage of development, then decreased as the leaf matured, and then increased during the latter stages of senescence. In the flesh of tomato fruits the contents of PPDK and PEPCK g−1 FW decreased during senescence. The results suggest that in fruits other than tomato the bulk of any gluconeogenic flux proceeds via PEPCK, whereas in tomato both PEPCK and PPDK could potentially be utilised. Further, the results indicate that the conversion of pyruvate/acetyl-CoA to malate by the glyoxylate cycle, for which ICL is necessary, is not a major pathway utilised by gluconeogenesis in fruits under normal conditions of growth. Finally, the results contribute to our understanding of the role of several enzymes in the senescence of both leaves and fruits.</p

Pilot report on the monitoring of the German bioeconomy

This report was prepared by the Center for Environmental Systems Research (CESR) of the University of Kassel and the Johann Heinrich von Thünen Institute (TI), Federal Research Institute for Rural Areas, Forests and Fisheries with the Specialist Institutes for Market Analysis (TI-MA) in Braunschweig, for International Forestry and Forest Economics (TI-WF) in Hamburg and for Sea Fisheries (TI-SF), together with partners of the SYMOBIO project.Overall coordination: Prof. Dr. Stefan Bringezu (CESR) in cooperation with Prof. Dr. Martin Banse (TI)German Federal Ministry of Education and Research (Grant number: 031B0281A

Cluster illumination differentially affects growth of fruits along their ontogeny in highbush blueberry (Vaccinium corymbosum L.).

Shading highbush blueberry plants generally leads to a delayed fruit development. Experiments have been performed to study the effects of light on fruit growth independently from the rest of the canopy. Clusters were shaded during different fruit growth periods. The equatorial diameter of the fruits as a function of days after full bloom followed a double-sigmoidal growth pattern, being fitted using a Gompertz II nonlinear mixed model, and absolute growth rates were obtained from each fitted model. Both whole-cycle shaded and second-stage shaded fruits showed a delayed peak in absolute growth curves with respect to both first-stage shaded and whole-cycle unshaded controls. Our results suggest that deficiency of light during the last stage of highbush blueberry fruits may lead to a substantial delay (of about 10–16 days) in harvest as compared with well-illuminated fruits. In order to estimate the contribution of intrinsic fruit photosynthesis to its own growth at different stages, clusters were subjected to girdling on their peduncles at different times. Girdling just before the second-stage resulted in fruits gaining between 35 and 40% of dry weight in comparison with the controls. This suggests that fruit photosynthesis may play a relevant role in fruit growth during the second sigmoidal stage, which in turn may contribute to explain the delayed growth observed in shaded fruits

Pilotbericht zum Monitoring der deutschen Bioökonomie

Der Pilotbericht umfasst die Ergebnisse des Forschungsprojekts SYMOBIO. Er wurde vom Center for Environmental Systems Research (CESR) der Universität Kassel und dem Johann Heinrich von Thünen-Institut (TI), Bundesforschungsinstitut für Ländliche Räume, Wald und Fischerei mit den Fachinstituten für Marktanalyse (TI-MA), für Internationale Waldwirtschaft und Forstökonomie (TI-WF) und für Seefischerei (TI-SF) zusammen mit Kooperationspartnern des SYMOBIO-Projekts erstellt. Gesamtkoordination: Prof. Dr. Stefan Bringezu (CESR) in Kooperation mit Prof. Dr. Martin Banse (TI)Gesamtkoordination: Prof. Dr. Stefan Bringezu (CESR) in Kooperation mit Prof. Dr. Martin Banse (TI)BMBF (Förderkennzeichen 031B0281A

National impact of the COVID-19 pandemic on clinical trial staff attrition: Results of the SWOG Cancer Research Network Survey of Oncology Research Professionals.

11049 Background: Severe shortages in clinical trial staffing across the United States and internationally has been anecdotally noted, but data are lacking. To better assess the scope and impact of staffing shortages, SWOG conducted a Cooperative Group-wide survey of Oncology Research Professionals (ORP). Methods: The survey was developed by SWOG leadership and was granted an IRB exemption by Lifespan IRB (Providence, RI). The survey was disseminated by email in January 2022 to Head Clinical Research Associates (CRAs, n = 100) using an inclusive distribution list that goes to the site-identified administrative leader of each SWOG Member and National Community Oncology Research Program institution. The data were collected and managed using REDCap electronic data capture tools hosted at Lifespan. Descriptive statistics were performed and qualitative analysis conducted to identify major themes. Results: The response rate was 87% and 41 of 87 respondents completed the full survey (47%). The majority of respondents were female (89.6%), not Hispanic (87.8%) and White (85.1%). The proportion that identified as Hispanic or Asian was 12.8 and 6.9%, respectively. One participant identified as Black and another as American Indian/Alaskan native. The most common work setting was within an academic medical center (47.9%) and 57.8% held a management or leadership role at their institutions. The majority (79%) used an Institutional IRB for trials not overseen as part of the National Clinical Trials Network. Over 80% of respondents reported their institution is experiencing a personnel shortage due to COVID-19. Proportion who reported this negatively impacted IRB processes was 50%, financial review was 42%, and legal review was 26.9%. On a scale of 0 (none) to 6 (significant), the impact was most significant on audit activities and accrual to trials (both rated 5), transfer of data to sponsors and sponsor visits (both rated 4.5); all other aspects rated a 4, including screening procedures, regulatory activities, and data collection. Ranked reasons for attrition were desire for better pay, seeking better opportunities, and seeking more flexible working conditions. General burn out was ranked as the fourth most common cause. Important themes included increasing trial complexity, morale, lack of support (due to staff shortages), lack of opportunities for promotion, unfilled positions, and the lack of experience of new hires. Conclusions: Over 80% of research programs affiliated with SWOG report staffing shortages due to the COVID-19 pandemic and the impact of these shortages touch every aspect of clinical research. Initiatives to recruit, train, and retain staff are urgently needed. As in other areas of medicine (e.g. hospital nursing), the potential for post-pandemic persistence of this issue requires an immediate national response. </jats:p