Gallstone Obstructive Ileus 3 Years Post-cholecystectomy to a Patient with an Old Ileoileal Anastomosis

The present case is one of gallstone obstructive ileus due to gallstones 3 yr after laparoscopic cholecystectomy. It is interesting because of the sex of the patient, the fact that ileus occurred 3 yr after cholecystectomy and that the localization of the obstruction was an old side-to-side ileoileal anastomosis due to a diverticulectomy following intussusception of Meckels' diverticulum at the age of 3

Small molecule inhibitors of hnRNPA2B1–RNA interactions reveal a predictable sorting of RNA subsets into extracellular vesicles

Extracellular vesicles (EVs) are cell-secreted membranous particles contributing to intercellular communication. Coding and noncoding RNAs can be detected as EV cargo, and RNA-binding proteins (RBPs), such as hnRNPA2B1, have been circumstantially implicated in EV-RNA sorting mechanisms. However, the contribution of competitive RBP–RNA interactions responsible for RNA-sorting outcomes is still unclear, especially for predicting the EV-RNA content. We designed a reverse proteomic analysis exploiting the EV-RNA to identify intracellular protein binders in vitro. Using cells expressing a recombinant hnRNPA2B1 to normalize competitive interactions, we prioritized a network of heterogeneous nuclear ribonucleoproteins and purine-rich RNA sequences subsequently validated in secreted EV-RNA through short fluorescent RNA oligos. Then, we designed a GGGAG-enriched RNA probe that efficiently interacted with a full-length human hnRNPA2B1 protein. We exploited the interaction to conduct a pharmacological screening and identify inhibitors of the protein–RNA binding. Small molecules were orthogonally validated through biochemical and cell-based approaches. Selected drugs remarkably impacted secreted EV-RNAs and reduced an RNA-dependent, EV-mediated paracrine activation of NF-kB in recipient cells. These results demonstrate the relevance of post-transcriptional mechanisms for EV-RNA sorting and the possibility of predicting the EV-RNA quality for developing innovative strategies targeting discrete paracrine functions

Osservazioni su alcuni aspetti di carattere filosofico e giuridico nel De carne Christi, nell'Adversus Marcionem e nel De exhortatione castitatis di Tertulliano

Il punto di partenza del contributo \ue8 il De natura deorum ciceroniano, di cui si studia la presenza in diversi scritti dell\u2019Africano, raccolti per rubriche tematiche. La prima \ue8 quella della realt\ue0 della carne di Cristo (Carn 5, 8-9 ; 23, 2 ; Marc 3, 8, 2), che il Cartaginese difende come reale nella sua costituzione materiale e nelle sue funzioni (egli si avvale a questo scopo dei testi di DND 1, 92 ; 1, 48-49). In altri passaggi il nostro autore polemizza contro diverse dottrine, di origine platonica o gnostica, sostenendo la veridicit\ue0 della percezione sensoriale (Marc 4, 8, 2-3 ; ma soprattutto An 17) : in questo caso l\u2019antecedente \ue8 il testo aristotelico, che veicola le dottrine dei presocratici (De An. 3, 3, 427 a). Si giunge quindi al tema del libero arbitrio, che Tertulliano afferma a pi\uf9 riprese contro Marcione (1, 22, 8 ; 2, 6, 1) : il modello \ue8 ciceroniano (DND 3, 76-78), ma dell\u2019argomento si studia anche la ricezione, dal De Genesi ad litteram di Agostino fino a Scoto Eriugena e Girolamo Balbi. Le argomentazioni sul libero arbitrio (Cast 2, 6 ; 3, 4) tornano anche nel testo agostiniano del De libero arbitrio

Caenorhabditis elegans BAH-1 Is a DUF23 Protein Expressed in Seam Cells and Required for Microbial Biofilm Binding to the Cuticle

The cuticle of Caenorhabditis elegans, a complex, multi-layered extracellular matrix, is a major interface between the animal and its environment. Biofilms produced by the bacterial genus Yersinia attach to the cuticle of the worm, providing an assay for surface characteristics. A C. elegans gene required for biofilm attachment, bah-1, encodes a protein containing the domain of unknown function DUF23. The DUF23 domain is found in 61 predicted proteins in C. elegans, which can be divided into three distinct phylogenetic clades. bah-1 is expressed in seam cells, which are among the hypodermal cells that synthesize the cuticle, and is regulated by a TGF-β signaling pathway