Gut microbial features can predict host phenotype response to protein deficiency.

Malnutrition remains a major health problem in low- and middle-income countries. During low protein intake, <0.67 g/kg/day, there is a loss of nitrogen (N2 ) balance, due to the unavailability of amino acid for metabolism and unbalanced protein catabolism results. However, there are individuals, who consume the same low protein intake, and preserve N2 balance for unknown reasons. A novel factor, the gut microbiota, may account for these N2 balance differences. To investigate this, we correlated gut microbial profiles with the growth of four murine strains (C57Bl6/J, CD-1, FVB, and NIH-Swiss) on protein deficient (PD) diet. Results show that a PD diet exerts a strain-dependent impact on growth and N2 balance as determined through analysis of urinary urea, ammonia and creatinine excretion. Bacterial alpha diversity was significantly (P < 0.05, FDR) lower across all strains on a PD diet compared to normal chow (NC). Multi-group analyses of the composition of microbiomes (ANCOM) revealed significantly differential microbial signatures between the four strains independent of diet. However, mice on a PD diet demonstrated differential enrichment of bacterial genera including, Allobaculum (C57Bl6/J), Parabacteroides (CD-1), Turicibacter (FVB), and Mucispirillum (NIH-Swiss) relative to NC. For instance, selective comparison of the CD-1 (gained weight) and C57Bl6/J (did not gain weight) strains on PD diet also demonstrated significant pathway enrichment of dihydroorodate dehydrogenase, rRNA methyltransferases, and RNA splicing ligase in the CD-1 strains compared to C57Bl6/J strains; which might account in their ability to retain growth despite a protein deficient diet. Taken together, these results suggest a potential relationship between the specific gut microbiota, N2 balance and animal response to malnutrition

RR Lyrae stars in four globular clusters in the Fornax dwarf galaxy

(Abridged) We have surveyed four globular clusters in the Fornax dwarf galaxy for RR Lyrae stars, using archival HST observations. We identify 197 new RR Lyrae stars in these four clusters. Despite the short observational baseline, we derive periods, light-curves, and photometric parameters for each. The Fornax clusters have exceptionally large RR Lyrae specific frequencies compared with the Galactic globular clusters. Furthermore, the Fornax cluster RR Lyrae stars are unusual in that their characteristics are intermediate between the two Galactic Oosterhoff groups. In this respect the Fornax clusters are similar to the field populations in several dwarf galaxies. We revise previous measurements of the HB morphology in each cluster. The Fornax clusters closely resemble the ``young'' Galactic halo population defined by Zinn. The existence of the second parameter effect among the Fornax clusters is also confirmed. Finally, we determine foreground reddening and distance estimates for each cluster. We find a mean distance modulus to Fornax of (m-M)_0 = 20.66 +/- 0.03 (random) +/- 0.15 (systematic). Our measurements are consistent with a line of sight depth of 8-10 kpc for this galaxy, matching its projected dimensions, and incompatible with tidal model explanations for the observed high velocity dispersions in many dSph galaxies. Dark matter dominance is suggested.Comment: 26 pages, 6 figures. Accepted for publication in MNRAS. Table 2 and Figure 2 will only be available in the electronic version. On-line data will soon be available at http://www.ast.cam.ac.uk/STELLARPOPS/Fornax_RRlyr

The Variable Stars and Blue Horizontal Branch of the Metal-Rich Globular Cluster NGC 6441

We present time-series VI photometry of the metal-rich ([Fe/H] = -0.53) globular cluster NGC 6441. Our color-magnitude diagram shows that the extended blue horizontal branch seen in Hubble Space Telescope data exists in the outermost reaches of the cluster. The red clump slopes nearly parallel to the reddening vector. A component of this slope is due to differential reddening, but part is intrinsic. The blue horizontal branch stars are more centrally concentrated than the red clump stars. We have discovered about 50 new variable stars near NGC 6441, among them eight or more RR Lyrae stars which are very probably cluster members. Comprehensive period searches over the range 0.2-1.0 days yielded unusually long periods (0.5-0.9 days) for the fundamental pulsators compared with field RR Lyrae of the same metallicity. Three similar long-period RR Lyrae are known in other metal-rich globulars. With over ten examples in hand, it seems that a distinct sub-class of RR Lyrae is emerging. The observed properties of the horizontal branch stars are in reasonable agreement with recent models which invoke deep mixing to enhance the atmospheric helium abundance, while they conflict with models which assume high initial helium abundance. The light curves of the c-type RR Lyrae seem to have unusually long rise times and sharp minima. Reproducing these light curves in stellar pulsation models may provide another means of constraining the physical variables responsible for the anomalous blue horizontal branch extension and sloped red clump observed in NGC 6441.Comment: 30 pages plus 6 EPS and 6 JPEG figures; uses AAS TeX. Accepted by the Astronomical Journal. Minor changes include computing He abundance, modifications to Figs 1 and 8, and expansion on idea that blue HB stars may be produced in binarie

Deep Photometry of the Globular Cluster M5: Distance Estimates from White Dwarf and Main Sequence Stars

We present deep VI photometry of stars in the globular cluster M5 (NGC 5904) based on images taken with the Hubble Space Telescope. The resulting color-magnitude diagram reaches below V ~ 27 mag, revealing the upper 2-3 magnitudes of the white dwarf cooling sequence, and main sequence stars eight magnitudes and more below the turn-off. We fit the main sequence to subdwarfs of known parallax to obtain a true distance modulus of (m-M)_0 = 14.45 +/- 0.11 mag. A second distance estimate based on fitting the cluster white dwarf sequence to field white dwarfs with known parallax yielded (m-M)_0 = 14.67 +/- 0.18 mag. We couple our distance estimates with extensive photometry of the cluster's RR Lyrae variables to provide a calibration of the RR Lyrae absolute magnitude yielding M_V(RR) = 0.42 +/- 0.10 mag at [Fe/H] = -1.11 dex. We provide another luminosity calibration in the form of reddening-free Wasenheit functions. Comparison of our calibrations with predictions based on recent models combining stellar evolution and pulsation theories shows encouraging agreement. (Abridged)Comment: AASTeX, 29 pages including 5 figures. Complete photometry data and FITS-format images are available at http://physics.bgsu.edu/~layden/ASTRO/PUBL/published.html . Accepted for publication in the Astrophysical Journal, 2005 October 20. Replaced errant wording in last sentence of paragraph 4 of conclusion

Stellar evolution through the ages: period variations in galactic RRab stars as derived from the GEOS database and TAROT telescopes

The theory of stellar evolution can be more closely tested if we have the opportunity to measure new quantities. Nowadays, observations of galactic RR Lyr stars are available on a time baseline exceeding 100 years. Therefore, we can exploit the possibility of investigating period changes, continuing the pioneering work started by V. P. Tsesevich in 1969. We collected the available times of maximum brightness of the galactic RR Lyr stars in the GEOS RR Lyr database. Moreover, we also started new observational projects, including surveys with automated telescopes, to characterise the O-C diagrams better. The database we built has proved to be a very powerful tool for tracing the period variations through the ages. We analyzed 123 stars showing a clear O-C pattern (constant, parabolic or erratic) by means of different least-squares methods. Clear evidence of period increases or decreases at constant rates has been found, suggesting evolutionary effects. The median values are beta=+0.14 day/Myr for the 27 stars showing a period increase and beta=-0.20 day/Myr for the 21 stars showing a period decrease. The large number of RR Lyr stars showing a period decrease (i.e., blueward evolution) is a new and intriguing result. There is an excess of RR Lyr stars showing large, positive $\beta$ values. Moreover, the observed beta values are slightly larger than those predicted by theoretical models.Comment: 15 pages, 9 figures; to be published in Astronomy and Astrophysics; full resolution version available at http://dbrr.ast.obs-mip.fr/tarot/publis/publis.htm

Systematics of RR Lyrae Statistical Parallax III: Apparent Magnitudes and Extinctions

We sing the praises of the central limit theorem. Having previously removed all other possible causes of significant systematic error in the statistical parallax determination of RR Lyrae absolute magnitudes, we investigate systematic errors from two final sources of input data: apparent magnitudes and extinctions. We find corrections due to each of ~0.05 mag, i.e., ~1/2 the statistical error. However, these are of opposite sign and so roughly cancel. The apparent magnitude system that we previously adopted from Layden et al. was calibrated to the photometry of Clube & Dawe. Using Hipparcos photometry we show that the Clube & Dawe system is ~0.06 mag too bright. Extinctions were previously pinned to the HI-based map of Burstein & Heiles. We argue that A_V should rather be based on new COBE/IRAS dust-emission map of Schlegel, Finkbeiner & Davis. This change increases the mean A_V by ~0.05 mag. We find M_V=0.77 +/- 0.13 at [Fe/H]=-1.60 for a pure sample of 147 halo RR Lyraes, or M_V=0.80 +/- 0.11 at [Fe/H]=-1.71 if we incorporate kinematic information from 716 non-kinematically selected non-RR Lyrae stars from Beers & Sommer-Larsen. These are 2 and 3 sigma fainter than recent determinations of M_V from main sequence fitting of clusters using Hipparcos measurements of subdwarfs by Reid and Gratton et al. Since statistical parallax is being cleared of systematic errors and since the chance of a >2 sigma statistical fluctuation is <1/20, we conclude that these brighter determinations may be in error. In the course of three papers, we have corrected 6 systematic errors whose absolute values total 0.20 mag. Had these, contrary to the expectation of the central limit theorem, all lined up one way, they could have resolved the conflict in favor of the brighter determinations. In fact, the net change was only 0.06 mag.Comment: submitted to ApJ, 21 pages, 2 tables, 4 figure

The anomalous Cepheid XZ Ceti

XZ Ceti is the only known anomalous Cepheid in the Galactic field. Being the nearest and brightest such variable star, a detailed study of XZ Ceti may shed light on the behaviour of anomalous Cepheids whose representatives have been mostly detected in external galaxies. CCD photometric and radial velocity observations have been obtained. The actual period and amplitude of pulsation were determined by Fourier analysis. The long time scale behaviour of the pulsation period was studied by the method of the O-C diagram using the archival Harvard photographic plates and published photometric data. XZ Ceti differs from the ordinary classical Cepheids in several respects. Its most peculiar feature is cycle-to-cycle variability of the light curve. The radial velocity phase curve is not stable either. The pulsation period is subjected to strong changes on various time scales including a very short one. The ratio of amplitudes determined from the photometric and radial velocity observations indicates that this Cepheid performs an overtone pulsation, in accord with the other known anomalous Cepheid in our Galaxy, BL Boo (V19 in the globular cluster NGC 5466). Continued observations are necessary to study the deviations from regularity, to determine their time scale, as well as to confirm binarity of XZ Ceti and to study its role in the observed peculiar behaviour.Comment: 7 pages, 4 figures. accepted for Astron. Astrophy