1,564 research outputs found

    Expression of related genes to the response of drought in Prunus rootstocks: abstract

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    PublishedPoster number 5

    Physiological characterization of drought stress response and expression of two transcription factors and two LEA genes in three Prunus genotypes

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    Global warming has led to a progressive decrease in rainfall, which is reflected by a reduction of water resources in the soil and a negative effect on crop production in Mediterranean areas. Under drought stress, many plants react by inducing a different series of responses at both physiological and molecular levels, allowing them to survive for a variable period of time. Therefore, in order to understand the response of roots to drought conditions, the genotypes peach × almond ‘Garnem’ [P. amygdalus Batsch × P. persica (L.) Batsch] and their progeny, the hybrid ‘P.2175’ × ‘Garnem’-3 and OP-‘P.2175’ (P. cerasifera Ehrh.) were subjected to a period of water deficit. Drought conditions with a subsequent re-watering period were tested for potted plants for one month. Stomatal conductance and leaf water potential were measured to monitor the plant physiological responses. Significant differences among the drought stress and drought stress recovery treatments and among the genotypes were observed. In addition, four genes related to the ABA biosynthesis pathway were studied for their expression by RT-qPCR: an AN20/AN1 zinc finger protein (ppa012373m); a bZIP transcription factor (ppa013046m); a dehydrin (ppa005514m) and a LEA protein (ppa008651m). Their expression profiles correlated with our physiological results of drought response, being higher in roots than in phloem tissue. In general, the expression of the four studied genes was higher after 15 days under drought conditions. Under drought and recovery conditions, the zinc finger and bZIP transcription factors showed significant differences in their relative expression levels from LEA and dehydrin. These results suggest the role of LEA and dehydrin in the regulatory response to drought stress in Prunus genotypes. Therefore, the dehydrin and the protein LEA might be potential biomarkers to select rootstocks for tolerance to drought conditions.We wish to thank Michael Glenn for helpful comments and ideas on the ash content discussion. This work was supported by RTA2014-00062 from the Instituto Nacional de Investigación y Tecnología Agraria (INIA) and by the Research Group A12 of Aragon, Spain. We kindly appreciate the FPI-INIA 2012 grant for B. Bielsa

    Faktor yang Berhubungan dengan Tindakan Merokok pada Mahasiswa Universitas Hasanuddin Makassar

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    Konsumsi rokok di Indonesia merupakan konsumsi rokok tertinggi ke lima di dunia. Secara nasional prevalensi penduduk umur di atas 15 tahun di Indonesia yang merokok setiap hari sebesar 28,2%. Merokok di kalangan remaja bisa merupakan bentuk tindakan awal dari penyalahgunaan narkoba, 90% pecandu narkoba bermula dari perokok pada usia muda. Penelitian bertujuan mengetahui faktor yang berhubungan dengan tindakan merokok pada mahasiswa Universitas Hasanuddin, Makassar. Jenis penelitian adalah penelitian analitik observasional dengan desain cross sectional study. Populasi adalah seluruh mahasiswa laki-laki dan perempuan pada Universitas Hasanuddin sebanyak 21.927orang. Sampel dalam penelitian ini adalah mahasiswa yang terpilih sebanyak 378 orang. Pengambilan sampel dengan cara proporsional stratified random sampling. Hasil penelitian menunjukkan responden yang masih merokok sebanyak 91 orang (24,1%). Hasil uji chi square menunjukkan semua variabel memiliki hubungan dengan tindakan merokok, yaitu pengetahuan (p=0,000, φ=0,232), sikap (p=0,000, φ=0,396), kemudahan mengakses rokok (p=0,000, φ=0,264), dukungan keluarga (p=0,044, φ=0,104), dukungan teman sebaya (p=0,000, φ=0,605), dan promosi/iklan rokok (p=0,000, φ=0,366).Kesimpulannya adalah faktor pengetahuan, sikap, kemudahan mengakses rokok, dukungan keluarga, dukungan teman sebaya, promosi/iklan memiliki hubungan dengan tindakan merokok

    Faktor Risiko Kegagalan Konversi pada Penderita Tuberkulosis Paru Bta Positif Baru di Kota Ambon Provinsi Maluku Tahun 2006

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    The objective of this study was to analyze risk factors of conversion failure of newly AFB positive TB patients at the end of intensive treatment in Ambon, Maluku. This study used case control design, in which the case consisted of all newly TB with AFB positive patients, aged more than 15 years, who experienced conversion failure (the AFB remained positive) at the end of intensive treatment and at the advance stage of treatment. The control consisted of all newly TB with AFB positive patients, aged more than 15 years, who did not experience conversion failure (the AFB has changed into negative) at the end of intensive treatment. Results showed that form eight variables assumed to have relations with conversion failure, five were found to have risk of conversion failure, i.e., Observer of treatment OR = 48,0 with (95% CI :15,20 151,54), Compliance to treatment OR= 41,8 with (95% CI : 9,45 184,72), and Drugs side effect OR = 9,37 with (95% CI : 4,08 21,49) Having identified risk factors of conversion failure, the author recommends the program managers to provide intensive information in order to improve the patients knowledge on pulmonary TB by involving Drugs Consumption Controller (DCC). In addition to direct promotion, other methods should also be employed, such as mass promotion and providing leaflets of booklets on TB. Concerning with treatment effectiveness, health care workers should educate the patients and DCC on drugs use, its importance, compliance to regular use, and actions that should be taken in case of side effects. All of these efforts will lead to reduced failure conversion rate and increased AFB negative sputum. Key Words : Lung Tuberculosis, DOTS, Intensive Phase, Sputum Conversion, Conversion Failure

    WORKSHOP PENDIDIK SEBAYA PUSAT INFORMASI DAN KONSELING KESEHATAN REPRODUKSI REMAJA HEART FKM UNHAS

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    Masih banyaknya masalah yang terkait dengan remaja khususnya pada mahasiswa yang terkait dengan seksualitas, NAPZA, HIV dan AIDS yang terjadi. Tujuan dari kegiatan ini adalah untuk meningkatkan pengetahuan, sikap, dan keterampilan pengelola PIK-KRM (Pendidik Sebaya) tentang program Kesehatan Reproduksi Remaja dalam rangka meningkatkan akses dan kualitas pelayanan PIK-KRM. Kegiatan Workshop Pendidik Sebaya PIK-KRM Heart FKM Universitas Hasanuddin dilaksanakan tiga hari pada tanggal 7 ??? 9 Oktober 2011 bertempat di Ruang Kandouw FKM Unhas yang diikuti oleh 40 orang peserta dan panitia yang merupakan anggota Pusat Informasi dan Konseling Kesehatan Reproduksi Mahasiswa Health Education of Reproductive Teenagers (PIK-KRM HEART). Adapun materi dan pemateri terdiri dari ; Wahiduddin, SKM, M.Kes (Bagian Epidemiologi FKM Unhas) materi yang disampaikan Kebijakan Program KRR, NAPZA dan Panduan Pendidik Sebaya. Ida Leida Maria, SKM, MKM, MScPH (Bagian Epidemiologi FKM Unhas) materi yang disampaikan Seksualitas dan HIV dan AIDS. Indra Fajawati Ibnu, SKM, MA (Bagian PKIP FKM Unhas) materi yang disampaikan Komunikasi. Wardihan, SE, MSi (BKKBN Provinsi Sulsel) materi yang disampaikan adalah Life Skills dan Pendewasaan Usia Perkawinan
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