COMPARATIVE ANALYSIS BASED ON SIMULATION FOR THE DESIGN OF LASER TERRESTRIAL MOBILE MAPPING SYSTEMS

International audienceOver the past decade, laser terrestrial Mobile Mapping Systems (MMS) have been developing for the digitizing of outdoor environments. While the applications of MMS are various (urban security, road control, virtual world, entertainment, etc.), one may imagine that for each application the system designs could be different. Hence, a comparative analysis of different designs may be useful to find the best solution adapted to each application. We present in this paper a methodology based on the use of a simulator, to compare several designs of MMS and to improve the design. We illustrate it in the case of urban architecture digitizing

In situ associations between marine photosynthetic picoeukaryotes and potential parasites - a role for fungi?

Photosynthetic picoeukaryotes (PPEs) are important components of the marine picophytoplankton community playing a critical role in CO2 fixation but also as bacterivores, particularly in the oligotrophic gyres. Despite an increased interest in these organisms and an improved understanding of the genetic diversity of this group, we still know little of the environmental factors controlling the abundance of these organisms. Here, we investigated the quantitative importance of eukaryotic parasites in the free-living fraction as well as in associations with PPEs along a transect in the South Atlantic. Using tyramide signal amplification-fluorescence in situ hybridization (TSA-FISH), we provide quantitative evidence of the occurrence of free-living fungi in open ocean marine systems, while the Perkinsozoa and Syndiniales parasites were not abundant in these waters. Using flow cytometric cell sorting of different PPE populations followed by a dual-labelled TSA-FISH approach, we also demonstrate fungal associations, potentially parasitic, occurring with both pico-Prymnesiophyceae and pico-Chrysophyceae. These data highlight the necessity for further work investigating the specific role of marine fungi as parasites of phytoplankton to improve understanding of carbon flow in marine ecosystems

Virus, bactéries et protistes pathogènes du phytoplancton, le rôle insoupçonné des parasites dans le fonctionnement des écosystèmes aquatiques

International audienceMicroscopiques, les parasites sont la plupart du temps invisibles, mais présents partout. Ils infectent tous les organismes du monde vivant. La dernière décennie a révélé une incroyable diversité chez les parasites viraux, bactériens et eucaryotes. Ceux infectant le phytoplancton pourraient avoir une importance capitale dans la dynamique des populations algales et dans le fonctionnement des écosystèmes aquatiques, mais leur rôle est encore très largement méconnu à ce jour (Brussaard, 2004). Sur ces questions, la recherche ne fait que commencer

Groups without cultured representatives dominate eukaryotic picophytoplankton in the oligotrophic South East Pacific Ocean

Background: Photosynthetic picoeukaryotes (PPE) with a cell size less than 3 µm play a critical role in oceanic primary production. In recent years, the composition of marine picoeukaryote communities has been intensively investigated by molecular approaches, but their photosynthetic fraction remains poorly characterized. This is largely because the classical approach that relies on constructing 18S rRNA gene clone libraries from filtered seawater samples using universal eukaryotic primers is heavily biased toward heterotrophs, especially alveolates and stramenopiles, despite the fact that autotrophic cells in general outnumber heterotrophic ones in the euphotic zone. Methodology/Principal Findings: In order to better assess the composition of the eukaryotic picophytoplankton in the South East Pacific Ocean, encompassing the most oligotrophic oceanic regions on earth, we used a novel approach based on flow cytometry sorting followed by construction of 18S rRNA gene clone libraries. This strategy dramatically increased the recovery of sequences from putative autotrophic groups. The composition of the PPE community appeared highly variable both vertically down the water column and horizontally across the South East Pacific Ocean. In the central gyre, uncultivated lineages dominated: a recently discovered clade of Prasinophyceae (IX), clades of marine Chrysophyceae and Haptophyta, the latter division containing a potentially new class besides Prymnesiophyceae and Pavlophyceae. In contrast, on the edge of the gyre and in the coastal Chilean upwelling, groups with cultivated representatives (Prasinophyceae clade VII and Mamiellales) dominated. Conclusions/Significance: Our data demonstrate that a very large fraction of the eukaryotic picophytoplankton still escapes cultivation. The use of flow cytometry sorting should prove very useful to better characterize specific plankton populations by molecular approaches such as gene cloning or metagenomics, and also to obtain into culture strains representative of these novel groups

SIMULATION BASED COMPARATIVE ANALYSIS FOR THE DESIGN OF LASER TERRESTRIAL MOBILE MAPPING SYSTEMS

Over the past decade, laser terrestrial Mobile Mapping Systems (MMS) have been developed for the digital mapping of outdoor environments. While the applications of MMS are various (urban security, road control, virtual world, entertainment, etc.), one may imagine that for each application the system designs could be different. Hence, a comparative analysis of different designs may be useful to find the best solution adapted to each application. The objective of this paper is to propose a methodology to compare point-cloud data quality from different MMS designs by modifying spatial configuration of laser imaging system. For this methodology, we define several quality criteria such as precision, resolution, completeness. We illustrate this in the case of urban architecture digital mapping based on the use of a simulator

Microbial eukaryote communities exhibit robust biogeographical patterns along a gradient of Patagonian and Antarctic lakes

Microbial eukaryotes play important roles in aquatic ecosystem functioning. Unravelling their distribution patterns and biogeography provides important baseline information to infer the underlying mechanisms that regulate the biodiversity and complexity of ecosystems. We studied the distribution patterns and factors driving diversity gradients in microeukaryote communities (total, abundant, uncommon and rare community composition) along a latitudinal gradient of lakes distributed from Argentinean Patagonia to Maritime Antarctica using both denaturing gradient gel electrophoresis (DGGE) and high-throughput sequencing (Illumina HiSeq). DGGE and abundant Illumina operational taxonomic units (OTUs) showed both decreasing richness with latitude and significant differences between Patagonian and Antarctic lakes communities. In contrast, total richness did not change significantly across the latitudinal gradient, although evenness and diversity indices were significantly higher in Patagonian lakes. Beta-diversity was characterized by a high species turnover, influenced by both environmental and geographical descriptors, although this pattern faded in the rare community. Our results suggest the co-existence of a ‘core biosphere’ containing reduced number of abundant/dominant OTUs on which classical ecological rules apply, together with a much larger seedbank of rare OTUs driven by stochastic and reduced dispersal processes. These findings shed new light on the biogeographical patterns and forces structuring inland microeukaryote composition across broad spatial scales

Rhizarian ‘Novel Clade 10’ Revealed as Abundant and Diverse Planktonic and Terrestrial Flagellates, including Aquavolon n. gen.

0000-0002-6719-5565The file attached is the Published/publisher’s pdf version of the articleThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited

An Approximation algorithm for scheduling Trees of Malleable Tasks

This work presents an approximation algorithm for scheduling the tasks of a parallel application. These tasks are considered as malleable tasks (MT in short), which means that they can be executed on several processors. This model receives recently a lot of attention, due mainly to their practical use for implementing actual parallel applications. Most of the works developed within this model deal with independent MT for which good approximation algorithms have been designed. This work is devoted to the case where MT are linked by precedence relations. We present a 1+epsilon approximation algorithm (for any fixed epsilon) for the specific structure of a tree. This preliminary result should open the way for further investigations concerning arbitrary precedence graphs of M

Measurements and calculations of H2O broadened by CO2 collisional parameters for planetary atmospheres

Collisional parameters of H2O with CO2 are currently missing from international spectroscopic databases, although they are essential for accurate modeling of water vapor in CO2-rich planetary atmospheres. In this study, high-resolution infrared spectra of H2O broadened by CO2 were recorded using a Fourier Transform Spectrometer in the 1.18 and 2.34 µm spectral regions. CO2-collisional parameters for selected H2O transitions were derived through a multispectrum fitting procedure employing both Voigt and quadratic speed-dependent Voigt profiles. Furthermore, calculations based on the semi-classical Complex Robert-Bonamy-Ma formalism were performed to estimate CO2-broadened half-widths, line shifts, and their temperature dependencies across various atmospheric transparency windows. It results in a strong agreement between theoretical predictions and experimental data. Finally, the shared calculated linelist, obtained for a wide range of transitions, can be directly applied to radiative transfer modeling of atmospheres primarily composed of carbon dioxide