Assessment of Changes in Body Composition After 3 Months of Dulaglutide Treatment [Letter]

Novaria Sari Dewi Panjaitan, Sarwo Handayani, Christina Safira Whinie Lestari Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Center, Bogor, West Java, IndonesiaCorrespondence: Novaria Sari Dewi Panjaitan, Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Jl. Raya Bogor No. 490, Cibinong – Bogor Km. 46, Bogor, West Java, Indonesia, Email [email protected]

Finite Element Analysis of Changes in Deformation of Intraocular Segments by Airbag Impact in Eyes of Various Axial Lengths [Letter]

Talitha Asmaria, Christina Safira Whinie Lestari, Monica Dwi Hartanti Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, West Java, IndonesiaCorrespondence: Talitha Asmaria, Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Jl. Raya Bogor No. 490, Bogor Km. 46, Cibinong, West Java, Indonesia, Email [email protected]

Operational Feasibility and Performance of mRPA Versus PCR for the Detection of HPV Types 16, 18, and 52 in Clinical Samples: An Exploratory Study

Monica Dwi Hartanti,1,2 Rahmi Amtha,3 Raditya Wratsangka,1 Astri Rinanti,4 Endang Djuana,5 Ilham Hizbulloh,1 Christopher Andrew Teguh,1 Alvionita Kogoya,1 Didik T Subekti,2 Muhammad Ibrahim Desem,2 Christina Safira Whinie Lestari,2 Talitha Asmaria2 1Faculty of Medicine, Universitas Trisakti, West Jakarta, Jakarta, Indonesia; 2Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong, West Java, Indonesia; 3Faculty of Dentistry, Universitas Trisakti, West Jakarta, Jakarta, Indonesia; 4Faculty of Landscape Architecture and Environmental Technology, Universitas Trisakti, West Jakarta, Jakarta, Indonesia; 5Faculty of Industrial Technology, Universitas Trisakti, West Jakarta, Jakarta, IndonesiaCorrespondence: Monica Dwi Hartanti, Faculty of Medicine, Universitas Trisakti, Jl. Kyai Tapa No. 260, West Jakarta, Jakarta, 11440, Indonesia, Email [email protected]: This exploratory study investigates the feasibility and performance of multiplex Recombinase Polymerase Amplification (mRPA) compared to conventional Polymerase Chain Reaction (PCR) for the detection and genotyping of high-risk Human Papillomavirus (HPV) types 16, 18, and 52. Current PCR methods are widely used for HPV detection but are limited by the need for complex thermal cycling equipment and lengthy processing times, which restrict their use in low-resource settings. This study aims to evaluate whether mRPA can serve as a faster, simpler, and more accessible alternative for HPV screening in primary healthcare environments.Methods: A total of 20 clinical samples from cervical swabs were tested using both mRPA and conventional PCR. The samples were preserved in ThinPrep® Specimen Collection fluid and stored at − 20°C. mRPA reactions were conducted under isothermal conditions at 39°C for 30 minutes, while conventional PCR followed standard cycling protocols. Sensitivity, specificity, operational efficiency, and feasibility in low-resource settings were assessed and compared between the two methods. The study complies with the Declaration of Helsinki and was approved by the Ethics Committee of Faculty of Medicine, Universitas Indonesia.Results: The mRPA demonstrated sensitivity and specificity that were lower than PCR, with detection rates of 100% for HPV 16, 80% for HPV 18, and 60% for HPV 52, compared to PCR’s 100% across all types. Overall, mRPA achieved an overall sensitivity of 80% and specificity of 100%. However, mRPA significantly reduced the amplification time to 30 minutes and eliminated the need for thermal cyclers, highlighting its potential suitability for primary healthcare settings. The practical implications of mRPA’s rapid turnaround time and simplified equipment requirements make it a promising tool for point-of-care applications in resource-limited environments.Conclusion: The findings suggest that mRPA could serve as a viable alternative to conventional PCR for HPV genotyping, offering advantages in speed and simplicity. Although mRPA’s diagnostic performance was lower than PCR, its operational benefits make it particularly suitable for use in resource-limited settings. Future research should focus on further optimization and validation to enhance mRPA’s diagnostic accuracy and explore its integration with user-friendly detection platforms.Keywords: HPV, mRPA, PCR, exploratory study, cervical cancer screening, genotypin

Burnout Syndrome Among Critical Care Health Providers in Saudi Arabia [Letter]

Sarwo Handayani, Christina Safira Whinie Lestari, Novaria Sari Dewi Panjaitan Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Center, Bogor, West Java, IndonesiaCorrespondence: Novaria Sari Dewi Panjaitan, Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Jl. Raya Bogor No. 490, Cibinong – Bogor Km. 46, Bogor, West Java, Indonesia, Email [email protected]