Global Analysis of Predicted G Protein-Coupled Receptor Genes in the Filamentous Fungus, Neurospora crassa.

G protein-coupled receptors (GPCRs) regulate facets of growth, development, and environmental sensing in eukaryotes, including filamentous fungi. The largest predicted GPCR class in these organisms is the Pth11-related, with members similar to a protein required for disease in the plant pathogen Magnaporthe oryzae. However, the Pth11-related class has not been functionally studied in any filamentous fungal species. Here, we analyze phenotypes in available mutants for 36 GPCR genes, including 20 Pth11-related, in the model filamentous fungus Neurospora crassa. We also investigate patterns of gene expression for all 43 predicted GPCR genes in available datasets. A total of 17 mutants (47%) possessed at least one growth or developmental phenotype. We identified 18 mutants (56%) with chemical sensitivity or nutritional phenotypes (11 uniquely), bringing the total number of mutants with at least one defect to 28 (78%), including 15 mutants (75%) in the Pth11-related class. Gene expression trends for GPCR genes correlated with the phenotypes observed for many mutants and also suggested overlapping functions for several groups of co-transcribed genes. Several members of the Pth11-related class have phenotypes and/or are differentially expressed on cellulose, suggesting a possible role for this gene family in plant cell wall sensing or utilization

Temporal Analysis of GRB Precursors in the Third Swift-BAT Catalog

Abstract We select 52 long gamma-ray bursts (GRBs) that have precursor activity in the third Swift-BAT catalog. Each episode shown in both the precursors and the main bursts is fitted by the Norris function. We systematically analyze the temporal properties for both the precursors and the main bursts. We do not find any significant difference between the temporal profile of the precursor and that of the main burst. The photon count of the precursor is related to that of the main burst. It is indicated that the precursor and the main burst might have the same physical origin, as the precursor and the main burst follow the same τ p –ω relation. However, we do not find the explicit relation between the energy release of the precursor and the quiescent time. Some theoretical models, such as the fallback collapsar scenario and the jet-cocoon scenario, may be helpful to explain the GRB-precursor phenomena.</jats:p