Nonlinear analysis of laminated shells with alternating stiff/soft lay-up

This paper proposes a multi-layer formulation for the nonlinear analysis of laminated shells with an alternating stiff/soft lay-up. The zigzag variation of planar displacements is taken into account by adding to the Reissner–Mindlin formulation a specific set of zigzag function which is effective for the considered lay-up. Furthermore, a piecewise linear through-thickness distribution of the material transverse shear strain is assumed, which agrees well with the real distribution. The proposed lamination model with a low-order nonlinear strain–displacement relationship is incorporated within a co-rotational framework for geometric nonlinear analysis, thus upgrading the low-order local element formulation to large displacement analysis with relative ease. In addition, a local shell system is employed for direct definition of the additional zigzag displacement fields and associated parameters, which are thus excluded from the large displacement co-rotational transformations. The application of the proposed laminated shell modelling approach is illustrated in this paper for a 9-noded co-rotational shell element, which utilises the Mixed Interpolation of Tensorial Components (MITC) method in the local system for overcoming locking effects. Several linear and nonlinear numerical examples of multi-layer shell structures with alternating stiff/soft lay-ups are used to illustrate the effectiveness and efficiency of the proposed modelling approach

A detailed analysis of online pharmacy characteristics to inform safe usage by patients

Background: Evidence suggests that consumers potentially put themselves at risk when purchasing medicines on-line. Whilst logos provided by regulators may provide some level of reassurance there may be other indicators which could be used by consumers to identify those websites which may be safely used. Objectives: Identify characteristics of on-line pharmacies which are related to whether websites are regulated or non-regulated and those characteristics which could be used by patients to increase the likelihood of accessing regulated sites. Setting: Online pharmacies which supply diazepam, fluoxetine and simvastatin. Methods: Using piloted search terms via Google and Yahoo search engines, identified websites were screened for regulatory status, adherence to regulatory standards, administrative requirements, clinical assessment requirements and additional details deemed to be of relevance to a user. Characteristics of regulated and non-regulated (defined as those with an absence of a correctly linked regulatory logo) websites were compared to identify differences which could be used to improve patient safety. Main outcome measure: Regulatory status, adherence to regulatory standards, quality of information provision, barriers to medicines access. Results: 113 websites sold diazepam, fluoxetine and simvastatin; were identified within the first 100 results. Less than quarter were found to be regulated online pharmacies. 80 websites were willing to sell the medication without a prescription. The unregulated internet pharmacy websites (defined as those with an absence of a correctly linked regulatory logo) were found to adhere more closely to the clinical criteria, were less significantly likely to disclose a contact name and address, telephone number of the pharmacy or demand a prescription prior to sale (P\0.05, Fisher’s Exact). Conclusions: The three prescription-only medicines which are liable to abuse, have potentially serious interactions and require counselling to ensure patient safety are readily available via the internet. When purchasing medicines via this route UK consumers should be made aware of the importance of regulatory logos and additionally should ensure that the seller can be meaningfully contacted by the contact details provided. The provision of clinical information should not be used alone as an indication of the seller’s provenance

Ispitivanje antioksidativne aktivnosti in vitro polipeptida meduze (Rhopilema esculentum)

Jellyfish gelatin was hydrolyzed by different proteases to obtain antioxidative polypeptides. The gelatin hydrolysate obtained by progressive hydrolysis using trypsin and Properase E exhibited the highest hydrolysis degree and antioxidant activity. Three series of gelatin polypeptides (SCP1, SCP2 and SCP3) were obtained by ultrafiltrating the gelatin hydrolysate through molecular mass cut-off membranes of 10, 6 and 2 kDa, respectively. Amino acid composition analysis showed that SCP3 had the highest total hydrophobic amino acid content. The in vitro antioxidant tests demonstrated that SCP2 had the strongest hydroxyl radical and hydrogen peroxide scavenging activities and metal chelating ability, while SCP3 showed the highest reducing power, antioxidant activity in linoleic acid emulsion system and superoxide anion radical scavenging activity. The results support the feasibility of jellyfish gelatin as a natural antioxidant polypeptide provider, and enzymatic hydrolysis and ultrafiltration could be potent future processing technologies to utilize the abundant jellyfish resource.Hidrolizom želatine iz meduze pomoću različitih proteaza dobiveni su polipeptidi antioksidativnih svojstava. Najveći stupanj hidrolize i najbolja antioksidativna svojstva imao je hidrolizat dobiven progresivnom hidrolizom pomoću tripsina i Properase E. Ultrafiltracijom hidrolizata kroz membrane (10, 6 i 2 kDa) dobivene su tri serije polipeptida želatine: SCP1, SCP2 i SCP3. Analizom aminokiselinskoga sastava ustanovljeno je da SCP3 ima najveći ukupni udio hidrofobnih aminokiselina. Antioksidativni testovi in vitro pokazali su da SCP2 ima najveću sposobnost uklanjanja hidroksilnih radikala i vodikova peroksida te keliranja metala, a SCP3 najveću redukcijsku moć, najbolju antioksidativnu aktivnost u emulzijskom sustavu linoleinske kiseline te sposobnost uklanjanja superoksidnih aniona. Rezultati potvrđuju da se želatina iz meduze može upotrijebiti kao izvor prirodnih antioksidativnih polipeptida, a enzimska hidroliza i ultrafiltracija kao postupci prerade za njezino iskorištavanje

Genome-wide association study in obsessive-compulsive disorder: results from the OCGAS.

Obsessive-compulsive disorder (OCD) is a psychiatric condition characterized by intrusive thoughts and urges and repetitive, intentional behaviors that cause significant distress and impair functioning. The OCD Collaborative Genetics Association Study (OCGAS) is comprised of comprehensively assessed OCD patients with an early age of OCD onset. After application of a stringent quality control protocol, a total of 1065 families (containing 1406 patients with OCD), combined with population-based samples (resulting in a total sample of 5061 individuals), were studied. An integrative analyses pipeline was utilized, involving association testing at single-nucleotide polymorphism (SNP) and gene levels (via a hybrid approach that allowed for combined analyses of the family- and population-based data). The smallest P-value was observed for a marker on chromosome 9 (near PTPRD, P=4.13 × 10(-)(7)). Pre-synaptic PTPRD promotes the differentiation of glutamatergic synapses and interacts with SLITRK3. Together, both proteins selectively regulate the development of inhibitory GABAergic synapses. Although no SNPs were identified as associated with OCD at genome-wide significance level, follow-up analyses of genome-wide association study (GWAS) signals from a previously published OCD study identified significant enrichment (P=0.0176). Secondary analyses of high-confidence interaction partners of DLGAP1 and GRIK2 (both showing evidence for association in our follow-up and the original GWAS study) revealed a trend of association (P=0.075) for a set of genes such as NEUROD6, SV2A, GRIA4, SLC1A2 and PTPRD. Analyses at the gene level revealed association of IQCK and C16orf88 (both P<1 × 10(-)(6), experiment-wide significant), as well as OFCC1 (P=6.29 × 10(-)(5)). The suggestive findings in this study await replication in larger samples

Accretion Disc Theory: From the Standard Model Until Advection

Accretion disc theory was first developed as a theory with the local heat balance, where the whole energy produced by a viscous heating was emitted to the sides of the disc. One of the most important new invention of this theory was a phenomenological treatment of the turbulent viscosity, known as ''alpha'' prescription, when the (r$\phi$) component of the stress tensor was approximated by ($\alpha$ P) with a unknown constant $\alpha$. This prescription played the role in the accretion disc theory as well important as the mixing-length theory of convection for stellar evolution. Sources of turbulence in the accretion disc are discussed, including nonlinear hydrodynamical turbulence, convection and magnetic field role. In parallel to the optically thick geometrically thin accretion disc models, a new branch of the optically thin accretion disc models was discovered, with a larger thickness for the same total luminosity. The choice between these solutions should be done of the base of a stability analysis. The ideas underlying the necessity to include advection into the accretion disc theory are presented and first models with advection are reviewed. The present status of the solution for a low-luminous optically thin accretion disc model with advection is discussed and the limits for an advection dominated accretion flows (ADAF) imposed by the presence of magnetic field are analysed.Comment: Roceeding of the Int. Workshop "Observational Evidence for Black Holes in the Universe". Calcutta, 11-17 January 1998. Kluwer Acad. Pu

3-D scattering of obliquely incident Rayleigh waves by a saturated alluvial valley in a layered half-space

In this paper, three-dimensional (3-D) scattering of obliquely incident Rayleigh waves by a saturated alluvial valley embedded in a layered half-space is studied in the frequency domain by using the indirect boundary element method. The total responses are assumed as the sum of the free-field responses and scattered-field responses. The free-field responses are calculated using the direct stiffness method and the scatter-field responses outside and inside of the valley are simulated by applying two sets of fictitious moving distributed loads and pore pressures on the interface of the valley. The amplitudes of the fictitious distributed loads and pore pressures are determined from the boundary conditions. The method is validated by comparing the results with the results published in the literature, and numerical results are obtained for a saturated valley embedded in a uniform saturated half-space and in a single elastic layer overlying elastic half-space. The effects of incident frequency, incident angle, porosity, drainage condition, and soil layers on the dynamic responses are discussed. It is found that 3-D scattering is different from the 2-D case. As the porosity decreased, the pore pressures around the valley became smaller but their oscillations became violent. The wave fields in a layered site are determined by the “dynamic interaction between valley and the layered half-space” and the dispersion characteristics of Rayleigh waves for the given layered-half-space

Metabolic analysis of the interaction between plants and herbivores

Insect herbivores by necessity have to deal with a large arsenal of plant defence metabolites. The levels of defence compounds may be increased by insect damage. These induced plant responses may also affect the metabolism and performance of successive insect herbivores. As the chemical nature of induced responses is largely unknown, global metabolomic analyses are a valuable tool to gain more insight into the metabolites possibly involved in such interactions. This study analyzed the interaction between feral cabbage (Brassica oleracea) and small cabbage white caterpillars (Pieris rapae) and how previous attacks to the plant affect the caterpillar metabolism. Because plants may be induced by shoot and root herbivory, we compared shoot and root induction by treating the plants on either plant part with jasmonic acid. Extracts of the plants and the caterpillars were chemically analysed using Ultra Performance Liquid Chromatography/Time of Flight Mass Spectrometry (UPLCT/MS). The study revealed that the levels of three structurally related coumaroylquinic acids were elevated in plants treated on the shoot. The levels of these compounds in plants and caterpillars were highly correlated: these compounds were defined as the ‘metabolic interface’. The role of these metabolites could only be discovered using simultaneous analysis of the plant and caterpillar metabolomes. We conclude that a metabolomics approach is useful in discovering unexpected bioactive compounds involved in ecological interactions between plants and their herbivores and higher trophic levels.

A hierarchic optimisation approach towards locking-free shell finite elements

A hierarchic optimisation approach is presented for relieving inaccuracies in conforming shell elements arising from locking phenomena. This approach introduces two sets of strain modes: (i) objective strain modes, defined in the physical coordinate system, and (ii) corrective strain modes, representing conforming strains enhanced with hierarchic strain modes. This leads to two alternative families of element, objective and corrective, both arising from minimising the difference between objective and corrective strains. Importantly, the proposed approach not only alleviates shear and membrane locking, but it also addresses locking arising from element distortion. The application of the proposed optimisation approach is demonstrated for a 9-noded quadrilateral Lagrangian shell element, where the membrane, bending and transverse shear strains are separately optimised, all within a local co-rotational framework that extends the element application to geometric nonlinear analysis. Several numerical examples, including cases with geometric and material nonlinearity, are finally presented to illustrate the effectiveness of the optimised 9-noded shell element in relieving the various sources of locking

Whole-genome association analysis of treatment response in obsessive-compulsive disorder.

Up to 30% of patients with obsessive-compulsive disorder (OCD) exhibit an inadequate response to serotonin reuptake inhibitors (SRIs). To date, genetic predictors of OCD treatment response have not been systematically investigated using genome-wide association study (GWAS). To identify specific genetic variations potentially influencing SRI response, we conducted a GWAS study in 804 OCD patients with information on SRI response. SRI response was classified as 'response' (n=514) or 'non-response' (n=290), based on self-report. We used the more powerful Quasi-Likelihood Score Test (the MQLS test) to conduct a genome-wide association test correcting for relatedness, and then used an adjusted logistic model to evaluate the effect size of the variants in probands. The top single-nucleotide polymorphism (SNP) was rs17162912 (P=1.76 × 10(-8)), which is near the DISP1 gene on 1q41-q42, a microdeletion region implicated in neurological development. The other six SNPs showing suggestive evidence of association (P<10(-5)) were rs9303380, rs12437601, rs16988159, rs7676822, rs1911877 and rs723815. Among them, two SNPs in strong linkage disequilibrium, rs7676822 and rs1911877, located near the PCDH10 gene, gave P-values of 2.86 × 10(-6) and 8.41 × 10(-6), respectively. The other 35 variations with signals of potential significance (P<10(-4)) involve multiple genes expressed in the brain, including GRIN2B, PCDH10 and GPC6. Our enrichment analysis indicated suggestive roles of genes in the glutamatergic neurotransmission system (false discovery rate (FDR)=0.0097) and the serotonergic system (FDR=0.0213). Although the results presented may provide new insights into genetic mechanisms underlying treatment response in OCD, studies with larger sample sizes and detailed information on drug dosage and treatment duration are needed