Bifurcation of Arabidopsis NLR Immune Signaling via Ca2+-Dependent Protein Kinases

Nucleotide-binding domain leucine-rich repeat (NLR) protein complexes sense infections and trigger robust immune responses in plants and humans. Activation of plant NLR resistance (R) proteins by pathogen effectors launches convergent immune responses, including programmed cell death (PCD), reactive oxygen species (ROS) production and transcriptional reprogramming with elusive mechanisms. Functional genomic and biochemical genetic screens identified six closely related Arabidopsis Ca2+-dependent protein kinases (CPKs) in mediating bifurcate immune responses activated by NLR proteins, RPS2 and RPM1. The dynamics of differential CPK1/2 activation by pathogen effectors controls the onset of cell death. Sustained CPK4/5/6/11 activation directly phosphorylates a specific subgroup of WRKY transcription factors, WRKY8/28/48, to synergistically regulate transcriptional reprogramming crucial for NLR-dependent restriction of pathogen growth, whereas CPK1/2/4/11 phosphorylate plasma membrane-resident NADPH oxidases for ROS production. Our studies delineate bifurcation of complex signaling mechanisms downstream of NLR immune sensors mediated by the myriad action of CPKs with distinct substrate specificity and subcellular dynamics

Molecular cloning and preliminary functional analysis of six RING-between-ring (RBR) genes in grass carp (Ctenopharyngodon idellus)

Ubiquitination is a post-translational modification of proteins that is widely present in eukaryotic cells. There is increasing evidence that ubiquitinated proteins play crucial roles in the immune response process. In mammals, RING-between-RING (RBR) proteins play a key role in regulating immune signaling as the important E3 ubiquitin ligases during ubiquitination. However, the function of RBR in fish is still unclear. In the present study, six RBR genes (RNF19A, RNF19B, RNF144AA, RNF144AB, RNF144B and RNF217) of grass carp (Ctenopharyngodon idellus) were cloned and characterized. Similar to mammals, all six members of RBR family contained RING, inbetween-ring (IBR) and transmembrane (TM) domains. These genes were constitutively expressed in all studied tissues, but the relative expression level differed. Following grass carp reovirus(GCRV) infection, the expression of six RBR genes in liver, gill, spleen and intestine significantly altered. Additionally, their expression in Ctenopharyngodon idellus kidney (CIK) cells was significantly increased after GCRV infection. And deficiency of RNF144B in CIK with small interference RNA (siRNA) up-regulated polyinosinic:polycytidylic acid poly(I:C))- induced inflammatory cytokines production, including 1FN-I, TNF-alpha, IL-6, and transcription factor IRF3, which demonstrated that RNF144B was a negative regulator of inflammatory cytokines. Our results suggested that the RBR might play a vital role in regulating immune signaling and laid the foundation for the further mechanism research of RBR in fishes

Ligand-induced monoubiquitination of BIK1 regulates plant immunity

The detection of microorganism-associated ligands by plant cells activates a signalling cascade in which the kinase BIK1 is monoubiquinated, released from the FLS2-BAK1 complex, and internalized by endocytosis. Recognition of microbe-associated molecular patterns (MAMPs) by pattern recognition receptors (PRRs) triggers the first line of inducible defence against invading pathogens(1-3). Receptor-like cytoplasmic kinases (RLCKs) are convergent regulators that associate with multiple PRRs in plants(4). The mechanisms that underlie the activation of RLCKs are unclear. Here we show that when MAMPs are detected, the RLCK BOTRYTIS-INDUCED KINASE 1 (BIK1) is monoubiquitinated following phosphorylation, then released from the flagellin receptor FLAGELLIN SENSING 2 (FLS2)-BRASSINOSTEROID INSENSITIVE 1-ASSOCIATED KINASE 1 (BAK1) complex, and internalized dynamically into endocytic compartments. The Arabidopsis E3 ubiquitin ligases RING-H2 FINGER A3A (RHA3A) and RHA3B mediate the monoubiquitination of BIK1, which is essential for the subsequent release of BIK1 from the FLS2-BAK1 complex and activation of immune signalling. Ligand-induced monoubiquitination and endosomal puncta of BIK1 exhibit spatial and temporal dynamics that are distinct from those of the PRR FLS2. Our study reveals the intertwined regulation of PRR-RLCK complex activation by protein phosphorylation and ubiquitination, and shows that ligand-induced monoubiquitination contributes to the release of BIK1 family RLCKs from the PRR complex and activation of PRR signalling

Mobicom Poster: Evaluating Location Predictors with Extensive Wi-Fi Mobility Data

A fundamental problem in mobile computing and wireless networks is the ability to track and predict the location of mobile devices. An accurate location predictor can significantly improve the performance or reliability of wireless network protocols, the wireless network infrastructure itself, and many applications in pervasive computing. These improvements lead to a better user experience, to a more cost-effective infrastructure, or both. Location prediction has been proposed in many areas of wireless cellular networks as a means of enhancing performance, including better mobility management, improved assignment of cells to location areas, more efficient paging, and call admission control. To the best of our knowledge, no other researchers have evaluated location predictors with extensive mobility data from real users. In this poster we compare the most significant domain-independent predictors using a large set of user mobility data collected at Dartmouth College. In this data set, we recorded for two years the sequence of wireless cells (Wi-Fi access points) frequented by more than 6000 users. We found that the simple Markov predictors performed as well or better than the more complicated LZ predictors, with smaller data structures

Evaluating Next Cell Predictors with Extensive Wi-Fi Mobility Data

Location is an important feature for many applications, and wireless networks can better serve their clients by anticipating client mobility. As a result, many location predictors have been proposed in the literature, though few have been evaluated with empirical evidence. This paper reports on the results of the first extensive empirical evaluation of location predictors, using a two-year trace of the mobility patterns of over 6,000 users on Dartmouth\u27s campus-wide Wi-Fi wireless network. The surprising results provide critical evidence for anyone designing or using mobility predictors. \par We implemented and compared the prediction accuracy of several location predictors drawn from four major families of domain-independent predictors, namely Markov-based, compression-based, PPM, and SPM predictors. We found that low-order Markov predictors performed as well or better than the more complex and more space-consuming compression-based predictors

Isolation and expression of grass carp toll-like receptor 5a (CiTLR5a) and 5b (CiTLR5b) gene involved in the response to flagellin stimulation and grass carp reovirus infection

Toll-like receptor 5 (TLR5), a member of Toll-like receptors (TLRs) family and is responsible for the bacterial flagellin recognition in vertebrates, play an important role in innate immunity. In the study, two TLR5 genes of grass carp (Ctenopharyngodon idellus), named CiTLR5a and CiTLR5b, were cloned and analyzed. Both CiTLR5a and CiTLR5b are typical TLR proteins, including LRR motif, transmembrane region and TIR domain. The full-length cDNA of CiTLR5a is 3054 bp long, with a 2646 bp open reading frame (ORF), 78 bp 5' untranslated regions (UTR), and 330 bp 3' UTR. The full-length cDNA of CiTLR5b is 3326 bp, with a 2627 bp ORF, 95 bp 5' UTR, and 594 bp 3' UTR. Phylogenetic analysis showed that CiTLR5a and CiTLR5b were closed to the TLR5 of cirrhinus mrigala, cyprinus_carpio, and danio redo. Subcellular localization indicated that CiTLR5a and CiTLR5b shared similar localization pattern and may locate in the plasma membrane of transfected cells. Real-time quantitative PCR revealed CiTLR5a and CiTLR5b were constitutively expressed in all examined tissues, whereas the highest expressed tissue differed. Following exposure to flagellin and GCRV, CiTLR5a and CiTLR5b were up-regulated significantly. Moreover, the downstream genes of TLR5 signal pathway such as MyD88, NF-kappa B, IRF7, IL-1 beta, and TNF-alpha also up-regulated significantly, whereas the I kappa B gene was down-regulated, suggesting that CiTLR5a and CiTLR5b involved in response to flagellin stimulation and GCRV infection. The results obtained in the study would provide a new insight for further understand the function of TLR5 in teleost fish. (C) 2015 Elsevier Ltd. All rights reserved

Application of Fireproof Coating for New Energy Vehicle Battery Pack

In the development process of new energy vehicles, the battery pack is one of the key parts, and the safety of the battery pack has always been an important factor affecting the application range and market sales of new energy vehicles. In order to improve the safety of battery packs, fireproof coatings are widely used on the surface of battery packs. This paper introduces the application of fireproof coatings in new energy vehicles by analyzing the composition and function of fireproof coatings

It takes two to tango : molecular links between plant immunity and brassinosteroid signalling

In response to the invasion of microorganisms, plants actively balance their resources for growth and defence, thus ensuring their survival. The regulatory mechanisms underlying plant immunity and growth operate through complex networks, in which the brassinosteroid phytohormone is one of the central players. In the past decades, a growing number of studies have revealed a multi-layered crosstalk between brassinosteroid-mediated growth and plant immunity. In this Review, by means of the tango metaphor, we immerse ourselves into the intimate relationship between brassinosteroid and plant immune signalling pathways that is tailored by the lifestyle of the pathogen and modulated by other phytohormones. The plasma membrane is the unique stage where brassinosteroid and immune signals are dynamically integrated and where compartmentalization into nanodomains that host distinct protein consortia is crucial for the dance. Shared downstream signalling components and transcription factors relay the tango play to the nucleus to activate the plant defence response and other phytohormonal signalling pathways for the finale. Understanding how brassinosteroid and immune signalling pathways are integrated in plants will help develop strategies to minimize the growth-defence trade-off, a key challenge for crop improvement

Summary and future development of marine target surveillance based on spatial information network

The space information network is indispensable for marine target surveillance.Multi-satellite information fusion methods which are based on spatial computing mode can improve the ability of wide-area scanning,accurate recognition,persistent tracking and rapid responding.The mode of on-board information fusion for sea target surveillance needs to be further studied.Firstly,the developments of spatial information network and satellite for sea surveillance were discussed and the existing problems were summarized.Then the architecture of marine target surveillance based on spatial computing was put forward.The key technology and future development of on-board computing for ocean target surveillance were studied and discussed in the fields of spatial networking and information fusion

Inverse Kinematic Solutions of Dual Redundant Camera Robot Based on Genetic Algorithm

Inverse kinematic solutions for a dual redundant camera robot in position are examined in order to alleviate operation difficulty and reduce time. The inverse kinematic algorithm is based on a basic genetic algorithm, and the genetic algorithm which is used to solve the problem of a redundant robot is mainly optimized in the joint space. On this basis, the genetic algorithm improvement strategies are studied. In this paper, a genetic algorithm with constrained 2 redundant degrees of freedom (DOF) is proposed through setting 2 parameter variables, with more flexible structure of optimization objective function and more efficient algorithm than basic genetic algorithm. Finally, the result of inverse kinematic algorithm is achieved in terms of the physical prototype