Don Angus: Lanzamiento de productos y Organización de evento

Este trabajo de fin de grado (TFG) ha consistido en la Organización de un evento y el lanzamiento de productos para la empresa Don Angus. Esta propuesta tiene como objetivo la organización de dicho evento, basándonos en nueve pasos fundamentales como son: la designación de la titularidad del evento, especificación del carácter del acto, identificación de los objetivos, definición del mensaje, la elaboración de listados de invitados, armonización entre anfitrionazgo, presidencia y precedencias, elección del lugar y el momento, distribución de espacios y tiempos y evaluación final. La organización de este evento nos llevará a alcanzar dos objetivos secundarios. El aumento de la notoriedad de la empresa Don Angus, actualizando sus redes sociales y creando contenido específico y, posteriormente, alcanzar un mayor número de ventas. Apoyado todo esto como bien hemos mencionado por la presencia en redes sociales y en internet mediante la web creada.Universidad de Sevilla. Grado en Publicidad y Relaciones Pública

Diagnostic of Banana streak viruses (BSV) and study of their levels of prevalence and molecular diversity in Cuba

Banana and plantain play a significant role in food security in Cuba, as reflected by the important surfaces (ca 101 000 ha) devoted to their culture. Interspecific triploid (AAB) and tetraploid (AAAB) plantain hybrids harbouring both the Musa acuminata (A) and M. balbisnana (B) genomes are the most cultivated varieties, however triploid AAA dessert banana types such as Dwarf Cavendish and Yangambi Km 5 are also widely cultivated. All types are currently affected by Banana streak disease caused by several species of Banana streak virus [1]. This prompted a nationwide research effort focused on the occurrence, prevalence and diversity of BSV species in dessert and cooking banana. To this aim, leaf samples from symptomatic and asymptomatic plants of various genotypes (AAA, AAB, AAAB, AABB and ABB) were collected throughout Cuba and indexed for BSV species Goldfinger (BSGFV), Imové (BsImV), Mysore (BSMysV) and Obino l'Ewaï (BSOLV) by multiplex immunocapture PCR [2]. 33% of the 521 samples collected from plants harbouring only the A genome (AAA, AAAA) were found to be infected by one or several BSV species, with BSMysV the most prevalent species present in 45.3 % of the 172 infected samples. On the other hand, 39,4 % of the 1638 samples collected from interspecific hybrids (AAB, AAAB, ABB, AABB) were also found to be infected by one or several BSV species. However the presence of residual M. balbisiana genomic DNA in leaf extracts proved to interfere with multiplex immunocapture PCR and to lead to many false positives. This was confirmed by Southern blot analyses. Attempts to decrease contaminations by plant genomic DNA in PCR reactions were made by modulating the duration of immunocapture or by preventing the binding of plant genomic DNA to PCR tubes or plates. Using degenerate primers, viral sequences that appeared to belong to new BSV species were amplified from a limited number of plant samples. One of these sequences amplified from Dwarf Cavendish displayed 96 % identity with BSAcYunV. Southern blots performed using this amplified sequence as a probe confirmed the presence of this species in AAA dessert banana types. (Texte intégral

Chitin based hybrid composites reinforced with graphene derivatives: a nanoscale study

In this work, we present two novel nanostructured hybrid materials based on a chitin matrix loaded with increasing amounts of graphene oxide and reduced graphene oxide nanosheets (nGO and rGO, respectively). Both kinds of material (Chi:nGO and Chi:rGO) were studied using different spectroscopic and rheological techniques with the aim of understanding the interaction mechanism between chitin and nGO/rGO and explaining how the type of filler and its proportion affects its reinforcement. The production of these hybrids represents not only the obtention of low-cost materials with mechanical resistance but also a good opportunity for developing materials with several applications according to their composition. The nGO and rGO were characterised through FT-IR and ESR for the determination of the oxidation degree of each nanofiller. Then, the hybrids were spectroscopically analysed with FT-IR, ESR and SAXS which demonstrated that the components do not interact through covalent bonding and the nanosheets are well-dispersed among the chitin matrix. Finally, a rheological behavior assay was performed and its results were analysed in terms of G′ and η*. In short, all the results allowed us to conclude that nGO acts as a more efficient reinforcer than rGO due to the higher amount of hydrogen bonding established with chitin.Fil: González, Joaquín Antonio. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Villanueva, María Emilia. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Peralta Ramos, María Luz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); ArgentinaFil: Pérez, Claudio Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Mar del Plata. Instituto de Investigación en Ciencia y Tecnología de Materiales (i); ArgentinaFil: Piehl, Lidia Leonor. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Copello, Guillermo Javier. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Química Analítica y Fisicoquímica. Cátedra de Química Analítica Instrumental; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Metabolismo del Fármaco (i); Argentin

Lipid-based nanovesicles for nanomedicine

Molecular self-assembly has enabled the fabrication of biologically inspired, advanced nanostructures as lipid-based nanovesicles (L-NVs). The oldest L-NVs, liposomes, have been widely proposed as potential candidates for drug delivery, diagnostic and/or theranostic applications and some liposome-based drug products have already stepped from the lab-bench to the market. This success is attributed to their ability to encapsulate both hydrophobic and/or hydrophilic molecules, efficiently carry and protect them within the body and finally deliver them at the target site. These positive features are also coupled with high biocompatibility. However, liposomes still present some unsolved drawbacks, such as poor colloidal stability, short shelf-life, restricted and expensive conditions of preparation because of the inherent nature of their fundamental constituents (phospholipids). The new tools available in the self-assembly of controlled molecules have significantly advanced the field of L-NV design and synthesis, and nonliposomal L-NVs have been recently developed; this new generation of nanovesicles can represent a paradigm shift in nanomedicine: they may complement liposomes, showing their advantages and overcoming most of their drawbacks. Clearly, being still young, their rocky way to the clinic first and then to the market has just started and it is still long, but they have all the potentialities to reach their objective target. The purpose of this review is to first present the large plethora of L-NVs available, focusing on this new generation of non-liposomal L-NVs and showing their similarities and differences with respect to their ancestors (liposomes). Since the overspread of a nanomaterial to the market is also strongly dependent on the availability of technological-scale preparation methods, we will also extensively review the current approaches exploited for L-NV production. The most cutting-edge approaches based on compressed fluid (CF) technologies will be highlighted here since they show the potential to represent a game-change in the production of L-NVs, favouring their step from the bench to the market. Finally, we will briefly discuss L-NV applications in nanomedicine, looking also for their future perspectives.This work was financially supported by MINECO (DGI), Spain, grants BE-WELL CTQ2013-40480-R, TERARMET (RTC-2014-2207-1), QUATFORFRAG (RTC-2014-2183-5) and UNDERLIPIDS (RTC-2015-3303-1), and by AGAUR, Generalitat de Catalunya, “Grant 2014-SGR-17“. N.G. acknowledges the European Commission (EC) (FP7-PEOPLE-2013-Initial Training Networks (ITN) ‘‘NANO2FUN’’ Project No. 607721) for their Postdoctoral contract. The authors appreciate support from LIPOCELL project financed by CIBER-BBN and Praxis Pharmaceuticals and also acknowledge the financial support from Instituto de Salud Carlos III, through “Acciones CIBER”. The Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN) is an initiative funded by the VI National R&D&I Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund.Peer reviewe

Metodologías para la detección de especies de Banana streak virus en plátanos y bananos en Cuba

Los plátanos y bananos constituyen fuentes primordiales de alimentos para millones de personas en todo el mundo y en Cuba las áreas destinadas a estos cultivos ocupan una superficie de alrededor de 101 000 ha, constituyendo, los de mayor volumen de propagación a través del cultivo in vitro. La mayoría de las plantaciones están conformadas por híbridos ínterespecíficos, que con frecuencia aparecen infectados por Banana streak virus (BSV). Considerando la necesidad del control de la calidad durante los procesos de multiplicación y certificación de vitroplantas, el objetivo de nuestro trabajo consistió en determinar las especies de BSV que están presentes en Cuba mediante metodologías que permitirán el uso de donantes sanos destinados a la micropropagación del cultivo en todo el país. Para ello, se realizaron colectas de muestras sintomáticas o carentes de síntomas de BSV, de variedades pertenecientes a los grupos genómicos AAB, ABB, AAAB, AABB y AAA; en las provincias de Pinar del Río, La Habana, Villa Clara, Cienfuegos, Ciego de Ávila, Granma y Santiago de Cuba. La prevalencia de BSV en las diferentes variedades, se realizó a través de la reacción en cadena de la polimerasa con inmunocaptura y combinación de iniciadores (Multiplex IC-PCR). La utilización de cebadores específicos en la reacción de PCR, permitió la detección de secuencias episomales de las cuatro especies mas importantes de BSV (Goldfinger (BSGFV), Imové (BsImV), Mysore (BSMysV) y Obino l'Ewaï (BSOLV). La especie BSGFV resultó ser la más frecuente y apareció provocando infecciones episomales simples o mixtas con BSOLV y BSImV. Mediante la implementación de estos métodos se logrará el uso de donantes libres de BSV en el sistema nacional de obtención de vitroplantas. (Résumé d'auteur

Homeopathic Perovskite Solar Cells: Effect of Humidity during Fabrication on the Performance and Stability of the Device

Rapid degradation in humid environments is a major drawback of methylammonium lead iodide (CH3NH3PbI3), which is the archetypical component of perovskite solar cells. In this work, we have investigated the aging and degradation kinetics of CH3NH3PbI3 films and devices fabricated under controlled conditions as a function of relative humidity (RH) and compared their performance with those that were prepared under dry conditions. The aging and degradation kinetics is monitored by optical absorption and impedance spectroscopy measurements under monochromatic illumination at two different wavelengths. Aged devices show a substantial difference between the recombination rate under red and blue light illumination, which is attributed to the enhancement of local recombination routes upon aging. Interestingly, we observe that this feature is less pronounced in devices prepared under conditions of the highest RH of 50%. In general, we found that these devices keep their original electric properties and withstand a humid environment better. Chemical analysis by X-ray photoelectron spectroscopy reveals the presence of coordinating water in the CH3NH3PbI3 crystalline structure. This indicates that the presence of a small amount of water has a beneficial effect against degradation in a humid environment

The db/db Mouse : a Useful Model for the Study of Diabetic Retinal Neurodegeneration

Background: To characterize the sequential events that are taking place in retinal neurodegeneration in a murine model of spontaneous type 2 diabetes (db/db mouse). Methods: C57BLKsJ-db/db mice were used as spontaneous type 2 diabetic animal model, and C57BLKsJ-db/+ mice served as the control group. To assess the chronological sequence of the abnormalities the analysis was performed at different ages (8, 16 and 24 weeks). The retinas were evaluated in terms of morphological and functional abnormalities [electroretinography (ERG)]. Histological markers of neurodegeneration (glial activation and apoptosis) were evaluated by immunohistochemistry. In addition glutamate levels and glutamate/aspartate transporter (GLAST) expression were assessed. Furthermore, to define gene expression changes associated with early diabetic retinopathy a transcriptome analyses was performed at 8 week. Furthermore, an additional interventional study to lower blood glucose levels was performed. Results: Glial activation was higher in diabetic than in non diabetic mice in all the stages (p<0.01). In addition, a progressive loss of ganglion cells and a significant reduction of neuroretinal thickness were also observed in diabetic mice. All these histological hallmarks of neurodegeneration were less pronounced at week 8 than at week 16 and 24. Significant ERG abnormalities were present in diabetic mice at weeks 16 and 24 but not at week 8. Moreover, we observed a progressive accumulation of glutamate in diabetic mice associated with an early downregulation of GLAST. Morphological and ERG abnormalities were abrogated by lowering blood glucose levels. Finally, a dysregulation of several genes related to neurotransmission and oxidative stress such as UCP2 were found at week 8. Conclusions: Our results suggest that db/db mouse reproduce the features of the neurodegenerative process that occurs in the human diabetic eye. Therefore, it seems an appropriate model for investigating the underlying mechanisms of diabetes-induced retinal neurodegeneration and for testing neuroprotective drugs

TFIIS is required for the balanced expression of the genes encoding ribosomal components under transcriptional stress

Transcription factor IIS (TFIIS) stimulates RNA cleavage by RNA polymerase II by allowing backtracked enzymes to resume transcription elongation. Yeast cells do not require TFIIS for viability, unless they suffer severe transcriptional stress due to NTP-depleting drugs like 6-azauracil or mycophenolic acid. In order to broaden our knowledge on the role of TFIIS under transcriptional stress, we carried out a genetic screening for suppressors of TFIIS-lacking cells’ sensitivity to 6-azauracil and mycophenolic acid. Five suppressors were identified, four of which were related to the transcriptional regulation of those genes encoding ribosomal components [rRNAs and ribosomal proteins (RP)], including global regulator SFP1. This led us to discover that RNA polymerase II is hypersensitive to the absence of TFIIS under NTP scarcity conditions when transcribing RP genes. The absence of Sfp1 led to a profound alteration of the transcriptional response to NTP-depletion, thus allowing the expression of RP genes to resist these stressful conditions in the absence of TFIIS. We discuss the effect of transcriptional stress on ribosome biogenesis and propose that TFIIS contributes to prevent a transcriptional imbalance between rDNA and RP genes.España Ministerio de Economía y competitividad BFU2007-67575-C03-02España Ministerio de Economía y competitividad BFU-2010-21975-C03-03Andalucía, Junta de Andalucía P07-CVI-02623Andalucía, Junta de Andalucía P08-CVI-035