Transient transgene expression of human coronavirus nl63 orf3 protein

>Magister Scientiae - MScInsect-derived baculoviruses have been used extensively as a safe and versatile research model for transgenic protein expression. Preclinical studies have revealed the promising potential of Baculoviruses as a delivery vector for a variety of therapeutic applications, including vaccination, tissue engineering and cancer treatments. Coronaviruses are enveloped viruses containing linear, non-segmented ribonucleic acid. Human coronavirus NL63 was first discovered in the Netherlands in January 2004, where a 7-month-old girl presented with an acute respiratory tract infection that was later established to predominantly infect infants, the elderly and immunocompromised individuals. In addition to the known non-structural and structural proteins of coronaviruses, an accessory protein known as open reading frame 3 which is conserved in the Coronaviridae family has not been extensively researched. Open reading frame 3 encodes a putative membrane-bound protein. This study cloned the open reading frame 3 viral gene of 741 base pairs into the baculovirus expression construct via competent bacterial cell lines. Open reading frame 3-Baculovirus particles were generated in Spodoptera frugiperda insect cells. Recombinant cells containing the viral protein gene were used to infect healthy Spodoptera frugiperda 9 cells at varying ratios of multiplicity of infection over a fixed time-course. The open reading frame 3 viral protein was not detected by quantification methods at a molecular weight of 26 kilo Dalton, due to polyclonal antibody degradation

Transient transgene expression of human Coronavirus nl63 orf3 protein in a baculovirus system

Magister Scientiae (Medical Bioscience) - MSc(MBS)Insect-derived baculoviruses have been used extensively as a safe and versatile research model for transgenic protein expression. Preclinical studies have revealed the promising potential of Baculoviruses as a delivery vector for a variety of therapeutic applications, including vaccination, tissue engineering and cancer treatments. Coronaviruses are enveloped viruses containing linear, non-segmented ribonucleic acid. Human coronavirus NL63 was first discovered in the Netherlands in January 2004, where a 7-month-old girl presented with an acute respiratory tract infection that was later established to predominantly infect infants, the elderly and immunocompromised individuals

Understanding the internal dynamics and organisation of Spaza shop operators

Magister Philosophiae - MPhilThis thesis presents a study of spaza shop businesses in the Delft South township, Cape Town, South Africa. The major goal is to establish whether the advent of foreign run spaza businesses is due to a particular ‘entrepreneurial’ business model underwritten by relatively strong social networks. The study focuses primarily on South African and Somali owned spaza shops as previous research indicates that these are the two major groups of spaza operators in the area. The thesis centres on three core research questions: 1) is there a shift in spaza ownership from South African to Somali shopkeepers in Delft? 2) What are the different spaza business models in operation? 3) What is the significance of social networks or relationships to the success of these business models? The core findings confirm that a major shift has occurred in market share between South African and foreign owned spazas in Delft, with ownership now favouring Somali businessmen, even within the last year. The research shows that this change in ownership is a direct result of the emergence and use of a new, and more sophisticated, ‘entrepreneurial’ business model employed by foreign spaza operators, compared to the more ‘survivalist’ model used by South Africans. This business model is primarily based upon being price competitive and is made possible through collective procurement and distribution. However, an important factor in this success lies in the differential social networks that South African and Somali spaza owners can access to support their business practices in Delft South. Using an anthropologically influenced in-depth ethnographic case study approach, the research operationalises the concept of ‘business models’ by exploring the establishment process (ownership, labour and employment), capital investment, stock procurement, business operation and mobile distribution to spaza shops. The study demonstrates how the socially richer and clan-based social networks of Somali shopkeepers enable a more entrepreneurial business model, whereas South Africans rely on a network limited to the immediate family and approach the spaza business as a supplementary livelihoods strategy. In addition to deepening our understanding of competing business models and the social networks that underwrite them, this research also provides new insights into the significance of spatiality to the spaza economy through the concepts of ‘strongholds’ and ‘neighbourhood economies’; previously unseen forms of spaza related business, principally around the mobile distribution of spaza stock to retailers in Delft South; and the instrumental use of both formality and informality by foreign business people

Protecting foreign children within South African borders: an evaluation into unaccompanied and undocumented foreign minors in South Africa

The main objective of the study is to investigate which policies are already in place pertaining to unaccompanied, separated and migrant children in South Africa. Children migrate to South Africa from neighbouring countries as Mozambique and Zimbabwe but also from countries as far away as the Democratic Republic of South Africa, without their parents or guardians. South Africa has developed legal and policy measures for securing the rights of children. Are these measures consistent with existing international frameworks and standards? Also, to what extent are these policies being used to resolve the problems unaccompanied children face? Related to this question is the issue of implementation. The thesis considers how the responsible departments and state officials such as Magistrates, social workers, police officials and the Department of Home Affairs implement these policies. This minor dissertation would then make some recommendations to the South African government

Understanding the internal dynamics and organisation of Spaza shop operators

Magister Philosophiae - MPhilThis thesis presents a study of spaza shop businesses in the Delft South township, Cape Town, South Africa. The major goal is to establish whether the advent of foreign run spaza businesses is due to a particular ‘entrepreneurial’ business model underwritten by relatively strong social networks. The study focuses primarily on South African and Somali owned spaza shops as previous research indicates that these are the two major groups of spaza operators in the area. The thesis centres on three core research questions: 1) is there a shift in spaza ownership from South African to Somali shopkeepers in Delft? 2) What are the different spaza business models in operation? 3) What is the significance of social networks or relationships to the success of these business models? The core findings confirm that a major shift has occurred in market share between South African and foreign owned spazas in Delft, with ownership now favouring Somali businessmen, even within the last year. The research shows that this change in ownership is a direct result of the emergence and use of a new, and more sophisticated, ‘entrepreneurial’ business model employed by foreign spaza operators, compared to the more ‘survivalist’ model used by South Africans. This business model is primarily based upon being price competitive and is made possible through collective procurement and distribution. However, an important factor in this success lies in the differential social networks that South African and Somali spaza owners can access to support their business practices in Delft South. Using an anthropologically influenced in-depth ethnographic case study approach, the research operationalises the concept of ‘business models’ by exploring the establishment process (ownership, labour and employment), capital investment, stock procurement, business operation and mobile distribution to spaza shops. The study demonstrates how the socially richer and clan-based social networks of Somali shopkeepers enable a more entrepreneurial business model, whereas South Africans rely on a network limited to the immediate family and approach the spaza business as a supplementary livelihoods strategy. In addition to deepening our understanding of competing business models and the social networks that underwrite them, this research also provides new insights into the significance of spatiality to the spaza economy through the concepts of ‘strongholds’ and ‘neighbourhood economies’; previously unseen forms of spaza related business, principally around the mobile distribution of spaza stock to retailers in Delft South; and the instrumental use of both formality and informality by foreign business people

Transient transgene expression of human coronavirus nl63 orf3 protein

>Magister Scientiae - MScInsect-derived baculoviruses have been used extensively as a safe and versatile research model for transgenic protein expression. Preclinical studies have revealed the promising potential of Baculoviruses as a delivery vector for a variety of therapeutic applications, including vaccination, tissue engineering and cancer treatments. Coronaviruses are enveloped viruses containing linear, non-segmented ribonucleic acid. Human coronavirus NL63 was first discovered in the Netherlands in January 2004, where a 7-month-old girl presented with an acute respiratory tract infection that was later established to predominantly infect infants, the elderly and immunocompromised individuals. In addition to the known non-structural and structural proteins of coronaviruses, an accessory protein known as open reading frame 3 which is conserved in the Coronaviridae family has not been extensively researched. Open reading frame 3 encodes a putative membrane-bound protein. This study cloned the open reading frame 3 viral gene of 741 base pairs into the baculovirus expression construct via competent bacterial cell lines. Open reading frame 3-Baculovirus particles were generated in Spodoptera frugiperda insect cells. Recombinant cells containing the viral protein gene were used to infect healthy Spodoptera frugiperda 9 cells at varying ratios of multiplicity of infection over a fixed time-course. The open reading frame 3 viral protein was not detected by quantification methods at a molecular weight of 26 kilo Dalton, due to polyclonal antibody degradation

Transient transgene expression of human Coronavirus nl63 orf3 protein in a baculovirus system

Magister Scientiae (Medical Bioscience) - MSc(MBS)Insect-derived baculoviruses have been used extensively as a safe and versatile research model for transgenic protein expression. Preclinical studies have revealed the promising potential of Baculoviruses as a delivery vector for a variety of therapeutic applications, including vaccination, tissue engineering and cancer treatments. Coronaviruses are enveloped viruses containing linear, non-segmented ribonucleic acid. Human coronavirus NL63 was first discovered in the Netherlands in January 2004, where a 7-month-old girl presented with an acute respiratory tract infection that was later established to predominantly infect infants, the elderly and immunocompromised individuals

Magic in the ancient Near East with special reference to ancient Israel

Thesis (MA)--Stellenbosch University, 2002.ENGLISH ABSTRACT: In this investigation an anthropological and comparative approach was employed in the study of magic in the ancient Near East. Firstly, a survey was presented with regard to anthropological theories throughout the nineteenth and twentieth centuries. This forms the background against which evidence on magic with respect to the cultures of the ancient Near East is investigated. Secondly, examples of magic in the Ancient Near East was discussed, with reference to Egypt, Mesopotamia and Hittite Anatolia. Reference was made to categories such as magic spells, objects, rituals and magical experts (magicians) and various examples were discussed. Thirdly, an analysis was made about the phenomenon of magic in ancient Israel. In this context magic plays a somewhat different role in comparison to its other ancient Near Eastern neighbours. It was shown that so-called miraculous actions, miracle workers (prophets) and other religious actions (curses and blessings) in the Hebrew Bible could definitely be associated with magic. The frequent prohibitions against magical practises furthermore suggest that magic was indeed been practiced in ancient Israel.AFRIKAANSE OPSOMMING: In hierdie ondersoek met betrekking tot magie in die ou Nabye Ooste word gebruik gemaak van 'n antropologiese en vergelykende benadering. Eerstens word 'n oorsig aangebied van antropologiese teorieë met betrekking tot magie in die negentiende en twintigste eeue. Dit vorm die agtergrond waarteen die verskynsel van magie in die ou Nabye Ooste ondersoek word. Tweedens word voorbeelde van magie in die ou Nabye Ooste ondersoek, met verwysing na Egipte, Mesopotamië en die Hetiete. Spesiale aandag word gegee aan kategorieë soos magiese spreuke, magiese objekte, rituele en magiese spesialiste. Dit word toegelig met verskillende toepaslike voorbeelde. Derdens word 'n ondersoek gedoen na die aard van magie in Oud-Israel. In hierdie konteks het magie ietwat van 'n ander rol vervul in vergelyking met die ander ou Nabye Oosterse bure. Daar word aangedui dat sekere wonderdade, wonderwerkers (profete), en ander religieuse aksies (vervloekinge en seënuitsprake) in die Hebreeuse Bybel met magie geassosieer kan word. Die vele verbiedinge teen die beoefening van magie is 'n duidelike aanduiding dat magie inderdaad in Israel gepraktiseer is

Using hand maps to understand how intersecting inequalities affect possibilities for community safety in Cape Town

Abstract Knowledge about what builds and what blocks pathways to accountability in South African townships could be strengthened by better understanding the role of intersectionality in these settings. This article describes our use of a novel hand mapping method to explore identity and inequality among a collective of community members from the township of Delft in Cape Town. We share new insights about the effect of intersecting inequalities on the capacity of activists and concerned young citizens to take part in building accountability for safer spaces in South Africa. Although we gained a deeper understanding about some of the ways that intersecting inequalities play out in the lives of Delft residents, we also learnt lessons about the limits to facilitated accountability struggles. Some of these struggles relate directly to the inequalities that this study has highlighted, and some reflect the highly fluid socioeconomic and political environment in which our work was situated. This article is divided into eight main sections. We begin by introducing the theory of intersectionality and provide a rationale for using participatory visual methods as tools for intersectionality research. We then summarize the study context and describe the hand mapping method that was used. The following four sections present our results through discussions of identity and personal experiences of inequality, unpacking intersecting inequalities, the possibilities and constraints of advancing accountability, and balancing accountability with the conditions of collective action. The paper ends with a summary of the insights and conclusions we have reached through facilitating this action research process.</jats:p