A gelatin/collagen/polycaprolactone scaffold for skin regeneration

Background A tissue-engineered skin substitute, based on gelatin (“G”), collagen (“C”), and poly(ε-caprolactone) (PCL; “P”), was developed. Method G/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. Two different GC:PCL ratios (1:8 and 1:20) were used. Results Differential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around 60 °C. Scanning electron microscopy showed that all biocomposites had similar fibrous structures. Good cytocompatibility was present in all G/C/P biocomposites when incubated with primary human epidermal keratinocytes (PHEK), human dermal fibroblasts (PHDF) and human adipose-derived stem cells (ASCs) in vitro. All G/C/P biocomposites exhibited similar cell growth and mechanical characteristics in comparison with C/P biocomposites. G/C/P biocomposites with a lower collagen content showed better cell proliferation than those with a higher collagen content in vitro. Due to reasonable mechanical strength and biocompatibility in vitro, G/C/P with a lower content of collagen and a higher content of PCL (GCLPH) was selected for animal wound healing studies. According to our data, a significant promotion in wound healing and skin regeneration could be observed in GCLPH seeded with adipose-derived stem cells by Gomori’s trichrome staining. Conclusion This study may provide an effective and low-cost wound dressings to assist skin regeneration for clinical use

Supplement_S1_The_immunofluorescent_staining_of_EPCs_of_CD31 - Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

Supplement_S1_The_immunofluorescent_staining_of_EPCs_of_CD31 for Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel by Niann-Tzyy Dai, Wen-Shyan Huang, Fang-Wei Chang, Lin-Gwei Wei, Tai-Chun Huang, Jhen-Kai Li, Keng-Yen Fu, Lien-Guo Dai, Pai-Shan Hsieh, Nien-Chi Huang, Yi-Wen Wang, Hsin-I Chang, Roxanne Parungao, and Yiwei Wang in Cell Transplantation</p