NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF- κ

The aim of the present study is to investigate the protective effects and relevant mechanisms exerted by NEMO-binding domain peptide (NBD) against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. The ALI model was induced by intratracheally administered atomized LPS (5 mg/kg) to BABL/c mice. Half an hour before LPS administration, we treated the mice with increasing concentrations of intratracheally administered NBD or saline aerosol. Two hours after LPS administration, each group of mice was sacrificed. We observed that NBD pretreatment significantly attenuated LPS-induced lung histopathological injury in a dose-dependent manner. Western blotting established that NBD pretreatment obviously attenuated LPS-induced IκB-α and NF-κBp65 activation and NOX1, NOX2, and NOX4 overexpression. Furthermore, NBD pretreatment increased SOD and T-AOC activity and decreased MDA levels in lung tissue. In addition, NBD also inhibited TNF-α and IL-1β secretion in BALF after LPS challenge. In conclusion, NBD protects against LPS-induced ALI in mice

Revolutionizing Eco-Friendly Leather Production: A Freeze-Thaw and Liquid Fermentation Approach with Fungal Mycelium

The environmental impact and resource demands of traditional leather manufacturing have driven the search for sustainable alternatives. Fungal mycelium leather, recognised for its eco-friendly and renewable characteristics, has emerged as a promising option. This study established a cyclic freeze-thaw dehydration protocol for preparing mycelial leather using Ganoderma mycelium produced through liquid fermentation. By precisely controlling the fermentation parameters (pH 5.5, 150 rpm agitation, 28 °C), the liquid fermentation process ensures uniform mycelial growth, which is critical for subsequent structural enhancement during freeze-thaw cycles. After three freeze-thaw cycles were performed at −15 °C, uniformly distributed ice crystals facilitated effective water removal, achieving a minimum moisture content of 47.6%. The optimized freeze-thaw process produced membranes with a tensile strength of 6.22 MPa and elongation at break of 18.92%, demonstrating high mechanical performance. The freeze-thaw process was demonstrated to enhance structural integrity and mechanical properties while offering reduced energy consumption compared to conventional dehydration methods. This research provides a theoretical foundation and technical guidance for optimising fungal mycelium leather production and contributes to the development of sustainable bio-based materials for industrial applications

Unexpected hydrazine hydrate-mediated aerobic oxidation of aryl/ heteroaryl boronic acids to phenols in ambient air

A general sub-stoichimetric hydrazine hydrate-mediated aerobic oxidative ipso-hydroxylation of aryl/heteroaryl boronic acids to phenols.</p

Online detection of part size based on laser contour scanner

Abstract In aerospace die manufacturing, the processing quality of the processed workpiece is an important guarantee for the quality of the final product. Traditional detection methods have high labor intensity and low efficiency. Visual detection can only detect width, not depth information. Based on this, this paper proposes a non-contact laser scanning point cloud processing quality online detection method, through the laser contour scanner to obtain the actual machining parts of the surface point cloud information, and then in the Geomagic Control environment of the actual manufacturing parts point cloud information and Solid Works theoretical design model comparison analysis. Thus, the manufacturing error of the actual manufacturing parts can be obtained, which provides a fast and accurate judgment method for the quality control of the product manufacturing.</jats:p

NEMO-Binding Domain Peptide Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Inhibiting the NF-κB Signaling Pathway

The aim of the present study is to investigate the protective effects and relevant mechanisms exerted by NEMO-binding domain peptide (NBD) against lipopolysaccharide- (LPS-) induced acute lung injury (ALI) in mice. The ALI model was induced by intratracheally administered atomized LPS (5 mg/kg) to BABL/c mice. Half an hour before LPS administration, we treated the mice with increasing concentrations of intratracheally administered NBD or saline aerosol. Two hours after LPS administration, each group of mice was sacrificed. We observed that NBD pretreatment significantly attenuated LPS-induced lung histopathological injury in a dose-dependent manner. Western blotting established that NBD pretreatment obviously attenuated LPS-induced IκB-α and NF-κBp65 activation and NOX1, NOX2, and NOX4 overexpression. Furthermore, NBD pretreatment increased SOD and T-AOC activity and decreased MDA levels in lung tissue. In addition, NBD also inhibited TNF-α and IL-1β secretion in BALF after LPS challenge. In conclusion, NBD protects against LPS-induced ALI in mice