Downregulation of miR-199b-5p is associated with chemoresistance via Jagged1 mediated notch signaling in human ovarian cancer cells

This journal suppl. entitled: 22nd Biennial Congress of the European Association for Cancer Research - proceedings of EACR 22BACKGROUND: Ovarian cancer is one of the most deadly gynaecological malignancies worldwide. Adjuvant chemotherapy combined with other treatments is the current management of the advanced ovarian cancers. However, the acquisition of resistance to initially responsive tumors limits the successful curative rate. The underlying molecular mechanism remains largely unknown. Here, we report that the downregulation of miR-199b-5p leading to elevated JAG1-mediated NOTCH signal...postprin

Thin Solid Electrolyte Layers Enabled by Nanoscopic Polymer Binding

To achieve high-energy all-solid-state batteries (ASSBs), solid-state electrolytes (SE) must be thin, mechanically robust, and possess the ability to form low resistance interfaces with electrode materials. Embedding an inorganic SE into an organic polymer combines the merits of high conductivity and flexibility. However, the performance of such an SE-in-polymer matrix (SEPM) is highly dependent on the microstructure and interactions between the organic and inorganic components. We report on the synthesis of a free-standing, ultrathin (60 μm) SEPM from a solution of lithium polysulfide, phosphorus sulfide, and ethylene sulfide (ES), where the polysulfide triggers the in situ polymerization of ES and the formation of Li3PS4. Reactant ratios were optimized to achieve a room-temperature conductivity of 2 × 10-5 S cm-1. Cryogenic electron microscopy confirmed a uniform nanoscopic distribution of β-Li3PS4 and PES (polyethylene sulfide). This work presents a facile route to the scalable fabrication of ASSBs with promising cycling performance and low electrolyte loading

Fracture analysis of bounded magnetoelectroelastic layers with interfacial cracks under magnetoelectromechanical loads: Plane problem

Fracture behaviors of multiple interfacial cracks between dissimilar magnetoelectroelastic layers subjected to in-plane magnetoelectromechanical loads are investigated by using integral transform method and singular integral equation technique. The number of the interfacial cracks is arbitrary, and the crack surfaces are assumed to be magnetoelectrically impermeable. The field intensity factors including stress, electric displacement and magnetic induction intensity factors as well as the energy release rates (ERRs) are derived. The effects of loading combinations, crack configurations and material property parameters on the fracture behaviors are evaluated according to energy release rate criterion. Numerical results show that both negative electrical and magnetic loads inhibit crack extension, and that the material constants have different and important effects on the ERRs. The results presented here should have potential applications to the design of multilayered magnetoelectroelastic structures. © The Author(s), 2010.postprin

Cloning of mouse integrin alphaV cDNA and role of the alphaV-related matrix receptors in metanephric development.

Metanephrogenesis has been a long-standing model to study cell-matrix interactions. A number of adhesion molecules, including matrix receptors (i.e., integrins), are believed to be involved in such interactions. The integrins contain alpha and beta s ubunits and are present in various tissues in different heterodimeric forms. In this study, one of the members of the integrin superfamily, alphaV, was characterized, and its relevance in murine nephrogenesis was investigated. Mouse embryonic renal cDNA libraries were prepared and screened for alphaV, and multiple clones were isolated and sequenced. The deduced amino acid sequence of the alpha-v cDNA clones and hydropathic analysis revealed that it has a typical signal sequence and extracellular, transmembrane, and cytoplasmic domains, with multiple Ca2+ binding sites. No A(U)nA mRNA instability motifs were present. Conformational analysis revealed no rigid long-range-ordered structure in murine alphaV. The alphaV was expressed in the embryonic kidney at day 13 of the gestation, with a transcript size of approximately 7 kb. Its expression increased progressively during the later gestational stages and in the neonatal period. It was distributed in the epithelial elements of developing nephrons and was absent in the uninduced mesenchyme. In mature metanephroi, the expression was relatively high in the glomeruli and blood vessels, as compared to the tubules. Various heterodimeric associations of alphaV, i.e., with beta1, beta3, beta5, and beta6, were observed in metanephric tissues. Inclusion of alphaV-antisense-oligodeoxynucleotide or -antibody in metanephric culture induced dysmorphogenesis of the kidney with reduced population of the nephrons, disorganization of the ureteric bud branches, and reduction of mRNA and protein expressions of alphaV. The expressions of integrin beta3, beta5, and beta6 were unaltered. These findings suggest that the integrin alphaV is developmentally regulated, has a distinct spatio-temporal expression, and is relevant in the mammalian organogenesis

Upregulation of chondroitin 6-sulphotransferase-1 facilities Schwann cell migration during axonal growth

Cell migration is central to development and posttraumatic regeneration. The differential increase in 6-sulphated chondroitins during axonal growth in both crushed sciatic nerves and brain development suggests that chondroitin 6-sulphotransferase-1 (C6ST-1) is a key enzyme that mediates cell migration in the process. We have cloned the cDNA of the C6ST-1 gene (C6st1) (GenBank accession number AF178689) from crushed sciatic nerves of adult rats and produced ribonucleotide probes accordingly to track signs of 6-sulphated chondroitins at the site of injury. We found C6st1 mRNA expression in Schwann cells emigrating from explants of both sciatic nerve segments and embryonic dorsal root ganglia. Immunocytochemistry indicated pericellular 6-sulphated chondroitin products around C6ST-1-expressing frontier cells. Motility analysis of frontier cells in cultures subjected to staged treatment with chondroitinase ABC indicated that freshly produced 6-sulphated chondroitin moieties facilitated Schwann cell motility, unlike restrictions resulting from proteoglycan interaction with matrix components. Sciatic nerve crush provided further evidence of in vivo upregulation of the C6ST-1 gene in mobile Schwann cells that guided axonal regrowth 1-14 days post crush; downregulation then accompanied declining mobility of Schwann cells as they engaged in the myelination of re-growing axons. These findings are the first to identify upregulated C6st1 gene expression correlating with the motility of Schwann cells that guide growing axons through both developmental and injured environments.published_or_final_versio

Acupuncture Treatment of Lateral Elbow Pain: A Nonrandomized Pilot Study

© 2016 Yan-Song Liu et al. In planning for a large-scale multicenter trial to evaluate the effect of acupuncture for the treatment of lateral elbow pain, a pilot study was conducted. This was a prospective, investigator- and patient-blinded, nonrandomized, placebo controlled trial. Subjects were evaluated at baseline, before fourth, seventh, and ninth treatment, and at a two-week posttreatment follow-up. The treatment group received unilateral acupuncture at LI 10 and LI 11 at the affected side with manual needle manipulation; the control group received sham-laser acupuncture at the same acupoints. Measures included (i) disabilities of the arm, shoulder, and hand (DASH) questionnaire, (ii) pain-free grip strength (PFGS), and (iii) a visual analogue scale (VAS) for pain. Significant differences in DASH score, PFGS, and VAS between treatment and control group were found at the ninth treatment (n = 20 for each group, P < 0.05). Only DASH showed significant differences compared to the control for all the measurement time points after treatment commenced and appears to be a sensitive and appropriate primary outcome measure for the future multisite trial. Results from this pilot study provided relevant information about treatment efficacy, credibility of control treatment, and sensitivity of different outcome measures for the planning of the future trial

DancingLines: An Analytical Scheme to Depict Cross-Platform Event Popularity

Nowadays, events usually burst and are propagated online through multiple modern media like social networks and search engines. There exists various research discussing the event dissemination trends on individual medium, while few studies focus on event popularity analysis from a cross-platform perspective. Challenges come from the vast diversity of events and media, limited access to aligned datasets across different media and a great deal of noise in the datasets. In this paper, we design DancingLines, an innovative scheme that captures and quantitatively analyzes event popularity between pairwise text media. It contains two models: TF-SW, a semantic-aware popularity quantification model, based on an integrated weight coefficient leveraging Word2Vec and TextRank; and wDTW-CD, a pairwise event popularity time series alignment model matching different event phases adapted from Dynamic Time Warping. We also propose three metrics to interpret event popularity trends between pairwise social platforms. Experimental results on eighteen real-world event datasets from an influential social network and a popular search engine validate the effectiveness and applicability of our scheme. DancingLines is demonstrated to possess broad application potentials for discovering the knowledge of various aspects related to events and different media

Reactive control of subsonic axial fan noise in a duct

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A semi-analytical method for bending, buckling, and free vibration analyses of sandwich panels with square-honeycomb cores

A semi-analytical method for bending, global buckling, and free vibration analyses of sandwich panels with square-honeycomb cores is presented. The discrete geometric nature of the square-honeycomb core is taken into account by treating the core sheets as thin beams and the sandwich panel as composite structure of plates and beams with proper displacement compatibility. Based on the classical model of sandwich panels, the governing equations of motion of the discrete structure are derived using Hamilton's principle. Closed-form solutions are developed for bending, global buckling, and free vibration of simply supported square-honeycomb sandwich panels by employing Fourier series and the Galerkin approach. Results from the proposed method agree well with available results in the literature and those from detailed finite element analysis. The effects of various geometric parameters of the sandwich panel on its behavior are investigated. The present method provides an efficient way of analysis and optimization of sandwich panels with square-honeycomb cores. © 2010 World Scientific Publishing Company.postprin

Structure-Induced Reversible Anionic Redox Activity in Na Layered Oxide Cathode

Anionic redox reaction (ARR) in lithium- and sodium-ion batteries is under hot discussion, mainly regarding how oxygen anion participates and to what extent oxygen can be reversibly oxidized and reduced. Here, a P3-type Na0.6[Li0.2Mn0.8]O2 with reversible capacity from pure ARR was studied. The interlayer O-O distance (peroxo-like O-O dimer, 2.506(3) Å), associated with oxidization of oxygen anions, was directly detected by using a neutron total scattering technique. Different from Li2RuO3 or Li2IrO3 with strong metal-oxygen (M-O) bonding, for P3-type Na0.6[Li0.2Mn0.8]O2 with relatively weak Mn-O covalent bonding, crystal structure factors might play an even more important role in stabilizing the oxidized species, as both Li and Mn ions are immobile in the structure and thus may inhibit the irreversible transformation of the oxidized species to O2 gas. Utilization of anionic redox reaction (ARR) on oxygen has been considered as an effective way to promote the charge-discharge capacity of the layered oxide cathodes for lithium- or sodium-ion batteries. The detailed mechanism of ARR, in particular how crystal structure affects and coordinates with the ARR, is not yet well understood. In the present work, a combination of X-ray and neutron total scattering measurements has been performed to study the structure of the prototype P3-type layered Na0.6[Li0.2Mn0.8]O2 with pure ARR. Unique structural characteristics, rather than prevailing knowledge of covalency of metal-oxygen, enable the stabilization of the crystal structure of Na0.6[Li0.2Mn0.8]O2 along with the ARR. This work suggests that reversible ARR can be manipulated by proper structure designs, thus to achieve high lithium or sodium storage in layered oxide cathodes. For P3-type Na0.6[Li0.2Mn0.8]O2 with relatively weak Mn-O covalent bonding, crystal structure factors play an important role in stabilizing the oxidized species, inhibiting the irreversible transformation of the oxidized species to O2 gas. The finding is important for better design of layered oxide positive materials with higher reversible capacity via the introduction of a reversible anionic redox reaction