Acute oral toxicity study of turmeric based herbal product in Sprague Dawley rats

The purpose of this study is to evaluate the acute oral toxicity study of the Turmeric based herbal product in the sprague dawley rats.  Acute oral toxicity refers to those adverse effects occurring following oral administration of a single dose of a substance, or multiple doses given within 24 hours. The LD50 value, defined as the statistically derived dose that, when administered in an acute toxicity test, is expected to cause death in 50% of the treated animals in a given period, is currently the basis for toxicologic classification of chemicals. The test item, Turmeric based herbal product was evaluated for Acute Oral Toxicity in Sprague Dawley Rats as per the OECD Guideline No. 425 -Acute Oral Toxicity - Up-and-Down Procedure. Based on the results, it may be concluded that the LD50 of test item is &gt;5000 mg/kg body weight as per OECD Guideline No. 425-Acute Oral Toxicity-Up-and-Down Procedure.</jats:p

Acute oral toxicity study of turmeric based herbal product in Sprague Dawley rats

The purpose of this study is to evaluate the acute oral toxicity study of the Turmeric based herbal product in the sprague dawley rats.&nbsp; Acute oral toxicity refers to&nbsp;those adverse effects occurring following oral administration of a single dose of a substance, or multiple doses given within 24 hours. The LD50 value, defined as the statistically derived dose that, when administered in an acute toxicity test, is expected to cause death in 50% of the treated animals in a given period, is currently the basis for toxicologic classification of chemicals. The test item, Turmeric based herbal product was evaluated for Acute Oral Toxicity in Sprague Dawley Rats as per the OECD Guideline No. 425 -Acute Oral Toxicity - Up-and-Down Procedure. Based on the results, it may be concluded that the LD50 of test item is &gt;5000 mg/kg body weight as per OECD Guideline No. 425-Acute Oral Toxicity-Up-and-Down Procedure

SPECROPHOTOMETRIC ESTIMATION AND STATISTICAL CORRELATION FOR ROSIGLITAZONE IN RAT AND HUMAN PLASMA

ABSTRACT   Objective: A rapid and sensitive Spectrophotometric method was developed for Rosiglitazone in rat and human plasma. Method: The sample was prepared by simple extraction method without derivatization and no use of buffer .Methanol and acetonitrile were used as the solvents in the proposed methods. Results: Calibration range extended for rosiglitazone from1mcg/ml to 10 mcg/ml in rat plasma and 1mcg/ml to 17mcg/ml in human plasma with good regression coefficients in both the cases. The Limit of Detection and Limit of Quantification were found out to be 0.726mcg/ml and 2.2mcg/ml in rat plasma and 0.414mcg/ml and 1.255mcg/ml in human plasma. Assay results from the proposed method were found to be 100.2% and 100.12% in rat and human plasma respectively. Stability of the drug in both the plasma was found to be suitable in both refrigerated and ambient conditions. Conclusion: The current method implied no significance difference as for estimation in rat and human plasma as tested from ANOVA analysis and can be extended pharmacokinetic studies. The proposed method was found prudent to be used in routine QC analysis. Keywords: Rosiglitazone, UV-Vis Spectrophotometer, Rat Plasma, Human Plasma, Statistical Correlatio