Study of the structural dynamics of laminin and cell-matrix interaction using high-speed atomic force microscopy

金沢大学博士(ナノ科学)博士論文　要旨Abstract/要約Outline 以下に掲載予定：Nature communications Nature portfolio. 共著者：Lucky Akter, Holger flechsig, Takao Arimori, Junichi Takagi, Clemens M. Franzdoctoral thesi

FARMERS’ USE OF MOBILE PHONE FOR COMMERCIAL FISH FARMING IN PANCHAGARH DISTRICT

A Thesis Submitted to the Department of Agricultural Extension and Information System Sher-e-Bangla Agricultural University, Dhaka In partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (MS) IN AGRICULTURAL EXTENSION AND INFORMATION SYSTEMThe key concern of this study was to explore the mobile phone usage behavior of fish farmers for commercial fish farming. Four unions of Atwari and Boda upazila under Panchagarh district were randomly selected as the locale of the study. One hundred (100) farmers were selected as the sample for this study. Data for this study were collected through personal interviews by the researcher herself from July 31 to August 31, 2021. Data were analyzed by Multiple Regression Analysis using SPSS 23.0. Age, level of education, farming experience, annual family income, fish farm size, communication exposure, fisheries training received, usefulness of using ICT, ICT selfefficacy, use of mobile phone were the selected variables for the study. Highest (45%) proportion of the fish farmers frequently used mobile phones for their commercial fish farming. Others were often, occasional, rare or not at all users of mobile phone for commercial fish farming. Considering the inferential statistics, education, communication exposure, ICT self-efficacy had positive and significant contributions to mobile phone use, constituting 70.5% (R 2 = 0.705) of the variance. Thus, the study concludes with the recommendation to enable a favorable environment to promote mobile phones in receiving fisheries production and its market information

PERFORMANCE OF HYBRID AND MODERN INBRED RICE VARIETIES UNDER AEROBIC CONDITION

Submitted to the Department of Agricultural Botany Sher-e-Bangla Agricultural University, Dhaka in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE (M.S.) IN AGRICULTURAL BOTANY SEMESTER: JANUARY-JUNE, 2019The field experiment was conducted at the Research Farm of Sher-e-Bangla Agricultural University, Dhaka during November, 2017 to June, 2018 to study the performance of hybrid and modern rice varieties under aerobic condition. The experimental treatments comprised of five varieties (V 1 = BRRI dhan 29, V 2 = Hybrid-3, V 3 = Moina, V 4 = Nobin and V = Hira-2) and different cultivation methods T 1 =SRI Method, T 2 = raised upland and T = Traditional). The experiment was laid out in Randomized Complete Block Design (RCBD) with 3 replications. The unit size of the plot was 4.0 m × 2.5 m. Data on plant height, number of tillers and leaves hill -1 3 were recorded at 45 DAT, 60 DAT, 75 DAT, 85 DAT and at harvest. And leaf length and leaf breath were at 60 DAT, 75 DAT and 85 DAT. The variety Nobin (V ) provided the highest significant performance in respect of plant height (36.24, 66.01, 82.94, 96.57 and 112.78 cm), number of tillers (6.58, 13.78, 15.58, 18.87 and 14.98), number of effective tillers hill 4 (13.56), leaves hill -1 (20.34, 44.29, 40.83, 54.84 and 50.28), leaf length (38.56, 35.56 and 48.20cm), leaf breath (0.88, 1.39 and 1.68cm), flag leaf length (34.18cm), flag leaf breadth (1.57cm), penultimate leaf length (41.68cm), dry weight of three leaves (0.63 g), penultimate leaf breadth (1.42cm), dry weight of leaves (16.48 g), chlorophyll content (50.36 mg g -1 ), dry stem weight (55.59 g), number of panicle (10.06), panicle height (101.22cm), panicle length (25.60 cm), panicle weight (17.91g), 1000-grain weight (26.14 g), highest grain yield (5.20 t ha ) and highest straw yield (5.20 t ha -1 ). T (traditional method) exhibited the highest significant performance in respect of number of tillers (6.65, 13.68,15.98,15.51 and 14.20), number of effective tillers hill (13.27), number of leaves hill 3 -1 5 -1 (21.13, 45.62, 41.15, 46.20 and 49.26), number of leaves hill (21.13, 45.62, 41.15, 46.20 and 49.26), leaf length (38.50, 35.70 and 46.03cm), leaf breadth (0.82, 1.35 and 1.64cm, flag leaf length (33.24cm) , flag leaf breadth (1.45cm) , penultimate leaf length (38.55cm), penultimate leaf breadth (1.44cm), dry weight of leaves (15.77g), dry weight of three leaves (0.63g), dry stem weight (51.04g), chlorophyll content (50.62 mg g ), number of panicle (8.40), panicle height (96.21cm), panicle weight (16.13g), 1000-grain weight (26.38g), highest grain yield (5.38 t ha -1 ) and highest straw yield (5.54t ha -1 ). The highest plant height obtained from T (raised upland) was recorded 35.44, 61.70, 76.51, 88.31 and 105.27cm. The maximum (1.60) number of effective tillers hill 2 -1 was obtained from T . There was significant effect of variety and aerobic condition. The interaction V 4 T 3 1 showed the highest significant performance in respect of plant height 37.97, 70.17, 51, 87.40, 101.23 and 115.67 cm, number of tiller (7.67, 17.57, 17.07, 19.97 and 18.33), number of effective tillers hill -1 (16.67), number of leaves hill -1 (23.00, 47.67, 53.07, 60.30 and 62.00), leaf length (42.33, 37.67 and 54.13cm), flag leaf length (39.80cm), flag leaf breadth (1.80cm), penultimate leaf length (44.00cm), penultimate leaf breadth (1.82cm), dry weight of leaves (20.50g), dry weight of three leaves (0.80g), dry stem weight (61.10g), chlorophyll content (61.00 mg g -1 ), number of panicle (11.17), panicle height (103.00cm), panicle length (26.73 cm), panicle weight (20.63g), 1000-grain weight (26.23g), highest grain yield (5.60 t ha -1 ) and highest straw yield (5.73 t ha -1 ). The highest leaf length of rice plants that received from V 3 T 3 . So, it may be concluded that the V (traditional) as singly or their interaction were more successful for produce the highest results. 4 -1 -1 (Nobin) and T -1 -1

Observing Dynamic Conformational Changes within the Coiled-Coil Domain of Different Laminin Isoforms Using High-Speed Atomic Force Microscopy

Laminins are trimeric glycoproteins with important roles in cell-matrix adhesion and tissue organization. The laminin alpha, ss, and gamma-chains have short N-terminal arms, while their C-termini are connected via a triple coiled-coil domain, giving the laminin molecule a well-characterized cross-shaped morphology as a result. The C-terminus of laminin alpha chains contains additional globular laminin G-like (LG) domains with important roles in mediating cell adhesion. Dynamic conformational changes of different laminin domains have been implicated in regulating laminin function, but so far have not been analyzed at the single-molecule level. High-speed atomic force microscopy (HS-AFM) is a unique tool for visualizing such dynamic conformational changes under physiological conditions at sub-second temporal resolution. After optimizing surface immobilization and imaging conditions, we characterized the ultrastructure of laminin-111 and laminin-332 using HS-AFM timelapse imaging. While laminin-111 features a stable S-shaped coiled-coil domain displaying little conformational rearrangement, laminin-332 coiled-coil domains undergo rapid switching between straight and bent conformations around a defined central molecular hinge. Complementing the experimental AFM data with AlphaFold-based coiled-coil structure prediction enabled us to pinpoint the position of the hinge region, as well as to identify potential molecular rearrangement processes permitting hinge flexibility. Coarse-grained molecular dynamics simulations provide further support for a spatially defined kinking mechanism in the laminin-332 coiled-coil domain. Finally, we observed the dynamic rearrangement of the C-terminal LG domains of laminin-111 and laminin-332, switching them between compact and open conformations. Thus, HS-AFM can directly visualize molecular rearrangement processes within different laminin isoforms and provide dynamic structural insight not available from other microscopy techniques

A STUDY ON DIFFERENT PLANTS OF APOCYNACEAE FAMILY AND THEIR MEDICINAL USES

The apocynaceae family is one of the most medicinally diverse families in the plant kingdom and is a rich source for drugs that have found use both traditionally and in conventional medicine. The medicinal activity of these plants was due to the presence of alkaloids which were either indoline alkaloids or steroidal alkaloids. The family Apocynaceae consists of tropical trees, shrubs and vines. Characteristic features of the family are that almost all species produce milky sap. In traditional medicine, Apocynaceae species are used to treat gastrointestinal ailments, fever, malaria, pain and diabetes, including skin and ecto-parasitic diseases. Some are important timber species while many are planted as ornamentals. Non-medicinal uses include food, poisons, fodder, wood, ornamentals, dye and perfume. A total of 4600 species under 415 genera belonging to the family Apocynaceae were collected and identified. Species of Apocynaceae have been reported to possess anticancer and antimalarial properties. Species having cytotoxic activity include those of Catharanthus, Nerium, Plumeria, Tabernaemontana and Ichnocarpus. Catharanthusroseus is the most medicinally important plant in this family due to its use in the treatment of various types of cancers, other agents that have been derived from this family include the alkaloids reserpine and rescinamine which have been used against hypertension, others are the cardiac glycosides.                      Peer Review History: Received 30 January 2019;   Revised 10 February; Accepted 1 March, Available online 15 March 2019 Academic Editor: Dr. Nuray Arı, Ankara University, Turkiye, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file:        Reviewer's Comments: Average Peer review marks at initial stage: 3.5/10 Average Peer review marks at publication stage: 6.5/10 Reviewer(s) detail: Dr. Mohamed Said Fathy Al-Refaey, University of Sadat City, Menofia, Egypt, [email protected] Prof. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, [email protected] Similar Articles: A REVIEW ON MEDICINAL USES OF DIFFERENT PLANTS OF EUPHORBIACEAE FAMIL

DEVELOPMENT AND IN VITRO DISSOLUTION STUDY OF BINARY AND TERNARY SOLID DISPERSIONS OF ACECLOFENAC

Objective: The poor aqueous solubility of the drug exhibits in variable dissolution rate and hence poor bioavailability. Aceclofenac is poorly water soluble drug. The aim of the present study was to improve the water solubility and the dissolution rate of Aceclofenac by solid dispersion technique using different water soluble polymers. The term solid dispersions refer to the dispersions of one or more active ingredients in an inert carrier or matrix at solid state. Methods: In this study, binary solid dispersion of Aceclofenac were prepared by fusion method using Polyethylene glycol 6000 (PEG 6000), Polyethylene glycol 4000 (PEG 4000), Poloxamer as carrier. Different drug-carrier weight ratio was used for this study. The effect of the carrier on the solubility and in-vitro dissolution were studied. Results: It was found the drug was released 26.86% after 5 minutes and only 40.19% within 60 mins from active Aceclofenac on the other hand the release pattern of Aceclofenac from the binary solid dispersion formulations containing PEG 6000 in 1:5 ratio (Formulation coding: A5) showed the best result in comparison of other binary and ternary solid dispersion formulations which was 62.29% after 5 min and 83.03% within 60 mins. The hydrophilic polymers used for the preparation of solid dispersion are showed significant increase in the solubility of Aceclofenac. Conclusion: This research showed that when Aceclofenac was dispersed in suitable water-soluble carriers such as PEG 6000, PEG 4000, Poloxamer, its dissolution were enhanced compared with pure drug.                                       Peer Review History: Received 20 February 2019;   Revised 28 February; Accepted 4 March, Available online 15 March 2019 Dr. Sally A. El-Zahaby, Pharos University in Alexandria, Egypt, [email protected] UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file:        Reviewer's Comments: Average Peer review marks at initial stage: 4/10 Average Peer review marks at publication stage: 7/10 Reviewer(s) detail: Dr. Jennifer Audu-Peter, University of Jos, Nigeria, [email protected] Dr. Areen Alshweiat, University of Szeged, Hungary, [email protected] Similar Articles: SOLID DISPERSION- STRATEGY TO ENHANCE SOLUBILITY AND DISSOLUTION OF POORLY WATER SOLUBLE DRUGS FABRICATION AND CHARACTERIZATION OF EZETIMIBE SOLID DISPERSION FOR SOLUBILITY ENHANCEMENT IN VITRO DISSOLUTION STUDY OF GLIMEPIRIDE FROM BINARY AND TERNARY SOLID DISPERSION FORMULATIO

Evaluation of anti-moisture effect of HPMC, Kollidon CL and Aerosil - 200 in hydrolysis affinity Clopidogrel-Aspirin tablet using Delta T Moister Sensor

The combined therapeutic effect of aspirin and clopidogrel is more potent than single one. This combined formulation is not stable due to their moisture affinity toward the hydrolysis reaction. The presence of moisture is a crucial factor in any formulation and causes sticking, picking, microbial growth, stability issues, lamination, friability. This combination formulation was designed using hydroxypropylmethyl cellulose 15 cps, Kollidon CL and Aerosil 200 in a controlled clean room class-I to stop the hydrolysis reaction. The tablets were made using slug method and hardness, disintegration, friability, dissolution, drug content, water activity, moisture content was determined. Kollidon CL and Aerosil 200 showed greater moisture reduction rate than HPMC 15 cps. The better anti-moisture effect of the excipient of Kollidon CL and Aerosil 200 was assured by the calculation of water activity using delta T sensor and isotherm, hysteresis. The quantity of excipients in the tablets was demonstrated for the significant affinity to moisture. The investigated simulated amount of Kollidon CL and Aerosil 200 showed the effective lower water activity in the delta T sensor. The mean moisture specification range was considered as 0.5-4.0% while the kollidon CL and Aerosol 200 showed moisture content of 1.32% and water activity (aw)of 0.014. In this study the Delta T sensor is used due to its significant reduction of dead time from 30% to 45% and moisture variation reduced at least 30% than other models treatment

Development of novel nanostructured biosensors for rapid detection of pathogens in clinical diagnostics

The prompt and precise identification of microorganisms is crucial for successful clinical diagnostics and the prevention of infectious disease outbreaks. Traditional diagnostic methods often suffer from limitations such as extended processing durations, elevated expenses, and the necessity for specialized laboratory equipment. In this research, we propose the development of novel nanostructured biosensors that utilize the distinct characteristics of nanomaterials to improve the accuracy, specificity, and efficiency of identifying pathogens. These biosensors are created with the intention of offering point-of-care testing functionality, thus rendering them appropriate for utilization in a range of clinical settings. The integration of advanced nanotechnology with bioanalytical methods aims to create a reliable system for the real-time identification of bacterial, viral, and fungal pathogens. This review encompasses the design, fabrication, and testing of the biosensors, along with a comprehensive analysis of their performance in comparison to conventional diagnostic techniques. The results demonstrate the potential of nanostructured biosensors to revolutionize pathogen detection, offering significant improvements in efficiency and accuracy, which are essential for timely medical intervention and public health management

ANTI-INFLAMMATORY AND ANALGESIC ACTIVITIES OF METHANOLIC SEED EXTRACT OF STERCULIA VILLOSA ROXB.

objective: To scientifically validate the analgesic and anti-inflammatory properties of methanolic seed extract of Sterculia villosa Roxb. (SVME) inanimal models.Methods: Analgesic activity of SVME was evaluated by the hot plate-induced pain model, acetic acid-induced abdominal writhing response, andformalin test in mice at the doses of 250 and 500 mg/kg. A carrageenan-induced paw edema model was also used to evaluate anti-inflammatorypotential of SVME in rats at doses of 250 and 500 mg/kg.Results: Phytochemical analysis revealed the presence of flavonoid, gums and carbohydrates, steroids, alkaloid, reducing sugar, and terpenoids insignificant amounts. The SVME produced significant analgesic activity in the hot-plate test in mice at all the time points measured. Extracts of 250 and500 mg/kg reduced dose-dependent acetic acid-induced writhing by 40.2% (p<0.01) and 59.8% (p<0.001), respectively. Significant inhibitionof formalin-induced pain was also observed, with inhibition of 62.1% (p<0.001) and 66.7% (p<0.001) in the early phase at dosages of 250 and500 mg/kg, respectively, and 64.4% (p<0.01) and 70.3% (p<0.01) in the late stage at these dosages. SVME pretreatments showed significant antiinflammatoryactivityagainstcarrageenan-inducededema at all the time points measured.Conclusion: The results suggest that SVME possesses analgesic and anti-inflammatory activities. These findings provide support use of this plant intraditional medicine to treat pain and inflammation.Keywords: Sterculia villosa Roxb., Analgesic, Anti-inflammatory, Carrageenan.Â