ALICE mesurements of heavy-flavour production in pp and p-Pb collisions at the LHC

The D meson yields as a function of charged-particle multiplicity in pp collisions at $\sqrt{s}=7$ TeV and in p-Pb collisions at $\sqrt{s_{\mathrm{NN}}}=5.02$ TeV are presented. The measurement of the yields of electrons from heavy-flavour hadron decays as a function of charged-particle multiplicity in p-Pb collisions at $\sqrt{s_{\mathrm{NN}}}=5.02$ TeV are shown as well. The measurement of azimuthal correlations of prompt D mesons and charged hadrons in pp collisions at $\sqrt{s}=7$ TeV and in p-Pb collisions at $\sqrt{s_{\mathrm{NN}}}=5.02$ TeV are also presented. The results are compared with expectations from models.Comment: 4 pages, 4 figures, proceedings of the 8th International Conference on Hard and Electromagnetic Probes of High-energy Nuclear Collisions, 23-27 September 2016, Wuhan, Chin

Measurement of D-meson production in p-Pb collisions with the ALICE detector

The ALICE Collaboration has measured the production of prompt D mesons in pPb collisions at sqrt(s_NN) = 5.02 TeV in the rapidity range -0.04 < y_{cms} < 0.96 via the exclusive reconstruction of their hadronic decays D0->K-pi+, D+->K- pi+ pi+, D*+->D0 pi+ and Ds->phi pi+. The pT-differential production cross sections and the pT-dependent nuclear modification factors with respect to a proton-proton reference, RpPb, are presented.Comment: Strangeness in Quark Matter 2013 proceedings, 4 pages, 2 figure

Bright spots in the darkness of cancer: A review of starfishes-derived compounds and their anti-tumor action

The fight against cancer represents a great challenge for researchers and, for this reason, the search for new promising drugs to improve cancer treatments has become inevitable. Oceans, due to their wide diversity of marine species and environmental conditions have proven to be precious sources of potential natural drugs with active properties. As an example, in this context several studies performed on sponges, tunicates, mollusks, and soft corals have brought evidence of the interesting biological activities of the molecules derived from these species. Also, echinoderms constitute an important phylum, whose members produce a huge number of compounds with diverse biological activities. In particular, this review is the first attempt to summarize the knowledge about starfishes and their secondary metabolites that exhibited a significant anticancer effect against different human tumor cell lines. For each species of starfish, the extracted molecules, their effects, and mechanisms of action are described

Future virialized structures: An analysis of superstructures in SDSS-DR7

We construct catalogues of present superstructures that, according to a LCDM scenario, will evolve into isolated, virialized structures in the future. We use a smoothed luminosity density map derived from galaxies in SDSS-DR7 data and separate high luminosity density peaks. The luminosity density map is obtained from a volume-limited sample of galaxies in the spectroscopic galaxy catalogue, within the SDSS-DR7 footprint area and in the redshift range 0.04 < z < 0.12. Other two samples are constructed for calibration and testing purposes, up to z = 0.10 and z = 0.15. The luminosity of each galaxy is spread using an Epanechnikov kernel of 8Mpc/h radius, and the map is constructed on a 1 Mpc/h cubic cells grid. Future virialized structures (FVS) are identified as regions with overdensity above a given threshold, calibrated using a LCDM numerical simulation, and the criteria presented by D\"unner et al. (2006). We assume a constant mass-to-luminosity ratio and impose the further condition of a minimum luminosity of 10^{12}Lsol. According to our calibrations with a numerical simulation, these criteria lead to a negligible contamination by less overdense (non FVS) superstructures.We present a catalogue of superstructures in the SDSS-DR7 area within redshift 0.04 < z < 0.12 and test the reliability of our method by studying different subsamples as well as a mock catalogue.We compute the luminosity and volume distributions of the superstructures finding that about 10% of the luminosity (mass) will end up in future virialized structures. The fraction of groups and X-ray clusters in these superstructures is higher for groups/clusters of higher mass, suggesting that future cluster mergers will involve the most massive systems. We also analyse known structures in the present Universe and compare with our catalogue of FVS.Comment: 14 pages, 11 figures, modified to match accepted version in MNRAS. PDF with high resolution colour figures is available at http://www.oac.uncor.edu/apache2-default/adminweb/html/WEB/preprints/2011.01/FVS-DR7.pd

Cytogenetic characterization of HB2 epithelial cells from the human breast

HB2 is a cell line originated by subcloning of MTSV1-7 mammary luminal epithelial cells isolated from human milk and immortalization via introduction of the gene encoding simian virus 40 (SV40) large T antigen. Despite its wide utilization as non-neoplastic counterpart in assays aimed to elucidating various biochemical and genetical aspects of normal and tumoral breast cells, to our knowledge no literature data have so far appeared concerning the chromosomal characterization of the HB2 cells. Here, we report the cytogenetic characterization of the karyotype of HB2 cells, which puts in evidence the occurrence of changes in chromosomal number and structure and the presence of unidentified chromosomal markers in variable amount. Our results do not detract from the utility of HB2 cells in illustrating fundamental aspects of breast cell biology, but rather interject a note of caution into generalizing results obtained with this cell line to other non-immortalized epithelial cell populations from the human breast. Therefore, this work represents a useful resource for all who want to perform appropriate and focused future studies on this cell line and proposes precise indications for a knowledgeable use of HB2 cells

Cytotoxicity of the urokinase-plasminogen activator inhibitor carbamimidothioic acid (4-boronophenyl) methyl ester hydrobromide (BC-11) on triple-negative MDA-MB231 breast cancer cells

Abstract: BC-11 is an easily synthesized simple thiouronium-substituted phenylboronic acid, which has been shown to be cytotoxic on triple negative MDA-MB231 breast cancer cells by inducing a perturbation of cell cycle when administered at a concentration equal to its ED50 at 72 h (117 μM). Exposure of cells to BC-11, either pre-absorbed with a soluble preparation of the N-terminal fragment of urokinase-plasminogen activator (uPa), or in co-treatment with two different EGFR inhibitors, indicated that: (i) BC-11 acts via binding to the N-terminus of the enzyme where uPa- and EGF receptor-recognizing sites are present, thereby abrogating the growth-sustaining effect resulting from receptor binding; and (ii) the co-presence of the EGFR inhibitor PD153035 potentiates BC-11’s cytotoxicity. Exposure of cells to a higher concentration of BC-11 corresponding to its ED75 at 72 h (250 μM) caused additional impairment of mitochondrial activity, the production of reactive oxygen species and promotion of apoptosis. Therefore, BC-11 treatment appears to show potential for the development of this class of compounds in the prevention and/or therapy of “aggressive” breast carcinoma

Effect of transfection with PLP2 antisense oligonucleotides on gene expression of cadmium-treated MDA-MB231 breast cancer cells

Emerging evidence indicates that cadmium (Cd) is able to regulate gene expression, drastically affecting the pattern of transcriptional activity in human normal and pathological cells. We have already shown that exposure of MDA-MB231 breast cancer cells to 5 uM CdCl2 for 96 h, apart from affecting significantly mitochondrial metabolism, induces modifications of the expression level of genes coding for members of stress response-, mitochondrial respiration-, MAP kinase-, NF-kB and apoptosis-related pathways. In the present study, we have expanded the knowledge on the biological effects of Cd-breast cancer cell interactions, indicating PLP2 (proteolipid protein-2) as a novel member of the list of Cd-upregulated genes by MDA-MB231 cancer cells and, through the application of transfection techniques with specific antisense oligonucleotides, we have demonstrated that such over-expression may be an upstream event to some of the changes of gene expression levels already observed in Cd-treated cells, thus unveiling new possible molecular relationship between PLP2 and genes linked to the stress- and apoptotic responses