97 research outputs found

    Aquaporin water channels in the nervous system.

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    The aquaporins (AQPs) are plasma membrane water-transporting proteins. AQP4 is the principal member of this protein family in the CNS, where it is expressed in astrocytes and is involved in water movement, cell migration and neuroexcitation. AQP1 is expressed in the choroid plexus, where it facilitates cerebrospinal fluid secretion, and in dorsal root ganglion neurons, where it tunes pain perception. The AQPs are potential drug targets for several neurological conditions. Astrocytoma cells strongly express AQP4, which may facilitate their infiltration into the brain, and the neuroinflammatory disease neuromyelitis optica is caused by AQP4-specific autoantibodies that produce complement-mediated astrocytic damage

    Increased proportion of nitric oxide synthase immunoreactive neurons in rat ileal myenteric ganglia after severe acute pancreatitis

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    <p>Abstract</p> <p>Background</p> <p>Severe acute pancreatitis (SAP) remains a potentially life-threatening disease. Gastrointestinal motility disturbance such as intestinal ileus is seen in every case. By now, the mechanisms of pancreatitis-induced ileus are largely unknown. The main purpose of the present study was to observe changes of nitric oxide synthase-immunoreactive (NOS-IR) neurons in ileal myenteric ganglia in SAP rats with gastrointestinal dysmotility, trying to explore underlying nervous mechanisms of pancreatitis-induced ileus.</p> <p>Methods</p> <p>Twenty Sprague Dawley rats were randomly divided into sham operated group and SAP group. SAP was induced by retrograde cholangiopancreatic duct injection of 5% sodium taurocholate. Abdominal X-ray and intestinal transit were performed to detect the existence of paralytic ileus and intestinal dysmotility. Pathological damage of pancreas was evaluated. Double-immunolabeling was employed for the whole-mount preparations of ileal myenteric ganglia. The morphology of NOS-IR neurons were observed and the percentage of NOS-IR neurons was calculated based on the total Hu-immunoreactive neurons. Total RNA of ileum was extracted according to Trizol reagent protocol. Neuronal NOS (nNOS) mRNA expression was evaluated by RT-PCR.</p> <p>Results</p> <p>The small intestinal transit index in the SAP group was significantly lower compared with the sham operated group (29.21 ± 3.68% vs 52.48 ± 6.76%, <it>P <</it>0.01). The percentage of NOS-IR neurons in ileal myenteric ganglia in the SAP group was significantly higher than that in the sham operated group (37.5 ± 12.28% vs 26.32 ± 16.15%, <it>P <</it>0.01). nNOS mRNA expression in ileum of SAP group was significantly higher than that in the sham operated group (1.02 ± 0.10 vs 0.70 ± 0.06, <it>P </it>< 0.01).</p> <p>Conclusions</p> <p>The increased quantity of NOS-IR neurons in ileal myenteric ganglia and increased nNOS mRNA expression may suggest nNOS over expression as one of the nervous mechanisms of gastrointestinal dysmotility in SAP rat.</p

    Migracja płazów oraz ich śmiertelność na lokalnej drodze w Parku Krajobrazowym Puszczy Knyszyńskiej

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    Spring migration and mortality of two common amphibian species on the road in one of the landscape parks in north-eastern Poland (Knyszyn Forest Landscpae Park) were studied in the years 2013&ndash;2015. During three years of the study, total number of 2745 individuals classified to 7 different species and one amphibian group were transfered through the road. The most popular species caught into the traps were: common toad, smooth newt and the common frog. Mortality of two studied amphibian species (Bufo bufoand rana temporaria) dropped over three years of the study. There were positive significant correlation between temperature and the number of migrating amphibians on the road

    Combat-ldentificationtechnieken

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    In dit artikel wordt een overzicht gegeven van de problematiek rondom het thema “eigen vuur” en de mogelijke maatregelen die men kan treffen om deze problematiek te verminderen. Daarbij ligt de focus op technologische maatregelen variërend van systemen die de situational awareness bevorderen door middel van het opbouwen en doorgeven van een common operational picture (COP), systemen voor gerichte target identification (TID) tot systemen voor noncooperative target recognition (NCTR). Het artikel beperkt zich tot technologieën die redelijk uitontwikkeld zijn. Elke categorie wordt kort toegelicht. Na deze beschrijving wordt kort ingegaan op niet-technologische maatregelen. Het artikel eindigt met een aantal conclusies rondom de mogelijke maatregele
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