Variación circadiana de los tiempos de tránsito T3 y T4 sobre el ajuste de las fases S, G2 y M en sistemas celulares parcialmente sincronizados

En el artículo se presenta un modelo continuo y determinista de la actividad proliferativa celular. Sobre dicho modelo básico se aplican sucesivos refinamientos que tienen por objeto mejorar el ajuste a datos experimentales existentes. La base experimental del método son los ensayos realizados sobre el pez Cauratus aclimatado a 25 grados con un fotoperiodo de 12 horas durante un mes. Se analizó la actividad proliferativa en las células intestinales observando que aquélla es parcialmente sincronizada. Las medidas efectuadas y su contraste con el método propuesto sugieren que el modelo determinista y contínuo es una aproximación adecuada a la interpretación del ciclo evolutivo. Los resultados numéricos sugieren un comportamiento circadiano también para los tiempos de tránsito en las fases. Las amplitudes se ajustan observando los mínimos de las curvas y la fase se induce mediante análisis armónico de la tendencia general

Reciclando conocimientos en TICs: herramientas online y offline para su utilización en la enseñanza virtual y en la coordinación docente

El profesorado de nuestra Facultad ha ido solicitando la realización de cursos formativos sobre las nuevas tecnologías y profundizar en las herramientas más habituales, sobre todo, con las posibilidades que ofrece el Campus Virtual. Con este proyecto se pretendieron cubrir fundamentalmente dos demandas: - Optimizar la utilización de las plataformas virtuales. - Conocer herramientas offline útiles para la docencia y la coordinación docente. En total, se han realizado 7 actividades manteniendo una estructura lógica de cronología y continuidad en las mismas. De la mayoría de ellas se han generado tutoriales como material de ayuda, que se han puesto a disposición del resto de profesores y PAS en un Seminario de Trabajo abierto creado en Moodle expresamente, denominado “PIMCD2014: Reciclando conocimientos…” (seminario-invest-97673-11)

Analysis of the existence of major genes affecting alpaca fiber traits

The aim of this study was to determine the presence of major genes for fiber diameter (FD), SD of FD (SDFD), CV of FD, and comfort factor (CF) in Huacaya (HU) and Suri (SU) Peruvian alpaca breeds. Bayesian segregation analyses with relaxed transmission probabilities were performed using 1,906 and 6,592 available records for SU and HU breeds. Evidence for the presence of major genes was statistically supported when the 95% posterior density did not include zero. Significant major genes were found associated with decreased FD, SDFD, CV values, and increased CF values. Additive effects of the major genes were 4.18 and 4.23 μm for FD, 1.67 and 1.61 μm for SDFD, 3.32 and 3.76% for CV, and 15.03 and 14.90% for CF in HU and SU breeds, respectively. Dominance effects were -1.98 and -2.03 μm for FD, -0.88 and -1.11 μm for SDFD, -1.37 and -2.17% for CV, and 13.0 and 11.8% for CF in HU and SU breeds, respectively. Major gene variance was larger than the polygenic variance for all traits. Major gene allelic frequencies for FD, SDFD, and CV ranged from 0.81 to 0.86 for HU breed and from 0.70 to 0.77 for the SU breed and were 0.24 and 0.36, respectively, for CF. It can be concluded that a major gene affecting these traits could be segregating. Then, molecular identification and monitoring of animals carrying favorable genes throughout the worldwide alpaca population would allow for a quick genetic improvement

Spectrometric characterization of amorphous silicon PIN detectors

During the last years, much interest has been dedicated to the use of amorphous silicon PIN diodes as particle and radiation detectors for medical applications. This work presents the spectrometric characterization of PECVD high deposition rate diodes fabricated at our laboratory, with thickness up to 17.5 μm. Results show that the studied devices detect the Am^(241) alpha particles and the medical X-rays generated by a mammograph model Senographe 700T from General Electric. Possible reasons of the observed energy losses are discussed in the lest. Using the SRIM2000 program, the transit of 5.5 MeV alpha particles through a diode was simulated, determining the optimum thickness for these particles to deposit their energy in the intrinsic layer of the diode

Ant-mediated seed dispersal in a warmed world

Climate change affects communities both directly and indirectly via changes in interspecific interactions. One such interaction that may be altered under climate change is the ant-plant seed dispersal mutualism common in deciduous forests of eastern North America. As climatic warming alters the abundance and activity levels of ants, the potential exists for shifts in rates of ant-mediated seed dispersal. We used an experimental temperature manipulation at two sites in the eastern US (Harvard Forest in Massachusetts and Duke Forest in North Carolina) to examine the potential impacts of climatic warming on overall rates of seed dispersal (using Asarum canadense seeds) as well as species-specific rates of seed dispersal at the Duke Forest site. We also examined the relationship between ant critical thermal maxima (CTmax) and the mean seed removal temperature for each ant species. We found that seed removal rates did not change as a result of experimental warming at either study site, nor were there any changes in species-specific rates of seed dispersal. There was, however, a positive relationship between CTmax and mean seed removal temperature, whereby species with higher CTmax removed more seeds at hotter temperatures. The temperature at which seeds were removed was influenced by experimental warming as well as diurnal and day-to-day fluctuations in temperature. Taken together, our results suggest that while temperature may play a role in regulating seed removal by ants, ant plant seed-dispersal mutualisms may be more robust to climate change than currently assumed

Soluble tumor necrosis factor receptor 1 and 2 predict outcomes in advanced chronic kidney disease : a prospective cohort study

Background : Soluble tumor necrosis factor receptors 1 (sTNFR1) and 2 (sTNFR2) have been associated to progression of renal failure, end stage renal disease and mortality in early stages of chronic kidney disease (CKD), mostly in the context of diabetic nephropathy. The predictive value of these markers in advanced stages of CKD irrespective of the specific causes of kidney disease has not yet been defined. In this study, the relationship between sTNFR1 and sTNFR2 and the risk for adverse cardiovascular events (CVE) and all-cause mortality was investigated in a population with CKD stage 4-5, not yet on dialysis, to minimize the confounding by renal function. Patients and methods : In 131 patients, CKD stage 4-5, sTNFR1, sTNFR2 were analysed for their association to a composite endpoint of all-cause mortality or first non-fatal CVE by univariate and multivariate Cox proportional hazards models. In the multivariate models, age, gender, CRP, eGFR and significant comorbidities were included as covariates. Results : During a median follow-up of 33 months, 40 events (30.5%) occurred of which 29 deaths (22.1%) and 11 (8.4%) first non-fatal CVE. In univariate analysis, the hazard ratios (HR) of sTNFR1 and sTNFR2 for negative outcome were 1.49 (95% confidence interval (CI): 1.28-1.75) and 1.13 (95% CI: 1.06-1.20) respectively. After adjustment for clinical covariables (age, CRP, diabetes and a history of cardiovascular disease) both sTNFRs remained independently associated to outcomes (HR: sTNFR1: 1.51, 95% CI: 1.30-1.77; sTNFR2: 1.13, 95% CI: 1.06-1.20). A subanalysis of the non-diabetic patients in the study population confirmed these findings, especially for sTNFR1. Conclusion : sTNFR1 and sTNFR2 are independently associated to all-cause mortality or an increased risk for cardiovascular events in advanced CKD irrespective of the cause of kidney disease

Genetic parameters and relationships between fibre and type traits in two breeds of Peruvian alpacas

Four fibre traits (fibre diameter, FD; coefficient of variation of FD, comfort factor; and standard deviation of FD) were jointly analysed with six subjectively scored type traits (fleece density, crimp, lock structure, head, coverage, and balance) in two breeds of Peruvian Altiplano alpaca (Suri, SU; and Huacayo, HU) to ascertain their genetic relationship. A total of 2405 fibre records and 2194 type scores were available for the HU breed whereas these figures were 709 for fibre records and 650 for type scores for the SU breed. Estimated heritabilities for fibre traits were moderate to high, ranging from 0.565 to 0.699 in the SU breed and from 0.255 to 0.417 in the HU breed. Genetic and permanent environmental correlations between fibre related traits were extremely similar across breeds suggesting that these traits are fairly the same. Heritabilities estimated for the type traits tended to be lower than those estimated for the fibre traits particularly in the SU breed (ranging from 0.173 to 0.272). Fibre and type traits were, in general, genetically poorly correlated except for crimp in the HU breed, which had favourable correlations, from moderate to high, for fibre traits. In Altiplano areas in which fibre performance recording could not be implemented, crimp scoring makes it feasible to carry out mass selection in the Huacayo breed and furthermore include rural communities in national or regional selection programmes

A TRAF2 binding independent region of TNFR2 is responsible for TRAF2 depletion and enhancement of cytotoxicity driven by TNFR1

Work at the authors laboratory was supported by Grant SAF2009-07227 from the Spanish Ministry of Science and Technology (MICINN) and by Grant IB09- 066 from the Plan Regional de Ciencia y Tecnología del Principado de Asturias (PCTI). LCH was recipient of fellowships from the PCTI. The IUOPA is supported by Obra Social Cajastur

Trisomy of a Down Syndrome Critical Region Globally Amplifies Transcription via HMGN1 Overexpression

Down syndrome (DS, trisomy 21) is associated with developmental abnormalities and increased leukemia risk. To reconcile chromatin alterations with transcriptome changes, we performed paired exogenous spike-in normalized RNA and chromatin immunoprecipitation sequencing in DS models. Absolute normalization unmasks global amplification of gene expression associated with trisomy 21. Overexpression of the nucleosome binding protein HMGN1 (encoded on chr21q22) recapitulates transcriptional changes seen with triplication of a Down syndrome critical region on distal chromosome 21, and HMGN1 is necessary for B cell phenotypes in DS models. Absolute exogenous-normalized chromatin immunoprecipitation sequencing (ChIP-Rx) also reveals a global increase in histone H3K27 acetylation caused by HMGN1. Transcriptional amplification downstream of HMGN1 is enriched for stage-specific programs of B cells and B cell acute lymphoblastic leukemia, dependent on the developmental cellular context. These data offer a mechanistic explanation for DS transcriptional patterns and suggest that further study of HMGN1 and RNA amplification in diverse DS phenotypes is warranted. How trisomy 21 contributes to Down syndrome phenotypes, including increased leukemia risk, is not well understood. Mowery et al. use per-cell normalization approaches to reveal global transcriptional amplification in Down syndrome models. HMGN1 overexpression is sufficient to induce these alterations and promotes lineage-associated transcriptional programs, signaling, and B cell progenitor phenotypes

Effects of parity order and reproductive management on the efficiency of rabbit productive systems.

The aim of this work was to study the effect of parity order and reproductive management systems on rabbit production performance. A total of 73 rabbit does (I group) were submitted to a 35-day intensive rhythm [artificial insemination (AI) on day 4 post-partum (pp) and weaning at 25 days of lactation], and 108 rabbit does (SI group) were submitted to a 42-day semi-intensive rhythm (AI on day 11 pp and weaning at 35 days of lactation) during 9 months. Primiparous does had the lowest live body weight at parturition (P < 0.0001) and at 21 days of lactation (P < 0.0001). They also had lower milk production (P < 0.0001) than does with later parities. I group animals needed a higher number of AI than SI group to become pregnant (1.70 ± 0.03 vs. 1.39 ± 0.03; P < 0.0001: especially after the third). Prolificacy was not affected by the management system. Parturition interval (PI) was longer than expected in both groups [56.0 ± 1.4 and 50.9 ± 1.38 days in I and SI groups, respectively (P < 0.05)]. Mean productivity, estimated as number of weaned rabbits per female and year, was 12 kits higher in rabbit does of the SI group (P < 0.05). From the third parturition onward, an increase in live body weight of kits at different ages was observed. At 21 (P < 0.05) and 25 days of age (P < 0.01), kits from the I group rabbit does weighed more than those from the SI group; however, the latter showed a higher weight at 35 (P < 0.05) and 60 days of age (P < 0.05). Rabbit does with two or three parturitions had higher litter size at 21 and 25 days of age (P < 0.0001 and P < 0.001, respectively). Kit mortality between 21 and 25 days of age and between 35 and 60 days of age was not affected by treatments but was higher in the I group between 25 and 35 days (18.2 vs. 5.03% in the I and SI groups, respectively; P < 0.0001) and as age of does increased (P < 0.05). In light of these results, we could conclude that long term doe reproductive performance is negatively affected and litter viability decreased when using intensive compared to a semi-intensive reproductive management