Morbilliviruses in aquatic mammals: report on round table discussion.

A workshop was organised to ascertain the current situation with regard to morbillivirus infections in aquatic animals. The great interest generated by the discovery of these new virus infections in 1988 has to some extent abated but much high quality research has continued in this field as the workshop showed. There is some serological evidence that the viruses have continued to circulate in most areas since the initial epizootics. As to their origin, it appears that the most likely source of the European seal morbillivirus (PDV-1) is the North Atlantic and Artic seal populations. As to the origin of the Mediterranean dolphin morbillivirus and the morbilliviruses isolated from porpoises, there is serological evidence that the viruses are widespread in many cetacean species in the Atlantic and 93% of long-finned pilot whales (Globicephala melas) which mass stranded between 1982 and 1993 were morbillivirus seropositive. The epizootic in freshwater seals in Lake Baikal was unrelated to events in the European marine mammal populations. The virus which infected these animals (PDV-2) is indistinguishable from canine distemper field strains. Serological and molecular biological studies provided evidence for the presence of the virus in the seals, at least as late as the Summer of 1992 when the animals were last sampled

Emerging and recurring diseases in cetaceans worldwide and the role of environmental stressors. Scientific Committee Document SC/60/DW5, International Whaling Commission, June 2008, Santiago, Chile

Emerging and recurring infectious diseases known or suspected to have the potential to significantly impact cetacean populations, and possible synergistic effects of environmental factors are reviewed. Cetacean morbilliviruses and papillomaviruses and brucellosis may affect population densities through high mortality rates or interference with reproduction. Evidence is available for the role of environmental factors in the emergence/recurrence and severity of at least six infectious conditions i.e. lobomycosis, toxoplasmosis, tattoo skin disease, generalized bacterial infections, miscellaneous skin diseases and morbillivirus epizootics. Other micro-parasites of potential importance include rhabdo-, herpes- and parainfluenza-viruses as well as Helicobacter spp., Streptococcus spp., Salmonella spp. and Mycobacterium marinum. The population impact and aetiology of newly emerging skin diseases in South America are unknown and represent a cause of concern

Entrepreneurial intention studies: A hybrid bibliometric method to identify new directions for theory and research

Fragmentation is the main obstacle to scientific progress on entrepreneurial intention. To address this issue, we systematise the current literature with a hybrid bibliometric method that combines co-citation and bibliographic coupling analysis for the first time in entrepreneurial intention studies to show the field's knowledge base and research fronts and to examine how divergent perspectives have challenged the core knowledge of the field. We highlight three recurring dimensions of entrepreneurial intention studies: (1) personal factors, (2) social factors and (3) investigational settings. In addition to introducing new constructs, divergent perspectives have emphasised the interplay between these components and challenged the mechanisms connecting them. Based on these findings, we extend previous classifications in the literature by providing a framework that integrates divergent perspectives with the field's knowledge base, helping establish future research avenues and improving the theorising process of entrepreneurial intention

Location of chlorogenic acid biosynthesis pathway and polyphenol oxidase genes in a new interspecific anchored linkage map of eggplant

© Gramazio et al.; licensee BioMed Central. 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated

EX-TRACT: An excel tool for the estimation of standard deviations from published articles

Meta-analysis, power analysis, and sensitivity analysis are widespread statistical techniques, which can be correctly performed only if variability statistics, such as standard deviation, are available; however, standard deviations are often missing in published articles. This work illustrates the functionality and the versatility of a newly developed Excel© tool for the standard deviation extraction from ANOVA and Multiple Comparison Test (MCT) results. The tool implements four methods, which can be alternatively applied according to the available statistics usually reported in ANOVA and/or MCT tables and graphs: 1) least significant difference (LSD), 2) significance level (p(F)), 3) letters for means separation assigned by MCT, 4) a range of significance level, indicated by "stars". The tool can be applied in one, two and three-way factorial experiments arranged in complete randomization, randomized block, split-plot or split-block. The performances of the different methods were tested in a case study about meta-analysis database preparation

Arsenic movement and fractionation in agricultural soils which received wastewater from an adjacent industrial site for 50 years

Arsenic (As) is an element with important environmental and human health implications due to its toxic properties. It is naturally occurring since it is contained in minerals, but it can also be enriched and distributed in the environment by anthropogenic activities. This paper reports on the historic As contamination of agricultural soils in one of the most important national relevance site for contamination in Italy, the so-called SIN Brescia-Caffaro, in the city of Brescia, northern Italy. These agricultural areas received As through the use of irrigation waters from wastewater coming from a factory of As-based pesticides (lead and calcium arsenates, sodium arsenite). Pesticide production started in 1920 and ended in the '70. Concentrations in the areas are generally beyond the legal threshold values for different soil uses and are up to >200 mg/kg. Arsenic contamination was studied to assess the long-time trend and the dynamics related to the vertical movement of As down to 1 m depth and its horizontal diffusion with surface irrigation in the entire field. Arsenic fractionation analysis (solid phase speciation by sequential extraction procedure) was also performed on samples collected from these areas and employed in greenhouse experiments with several plant species to evaluate the long-term contamination and the role of plant species in modifying As availability in soil. The results of this work can help in the evaluation of the conditions controlling the vertical transfer of As towards surface aquifers, the bioaccumulation likelihood in the agricultural food chain and the selection of sustainable remediation techniques such as phytoextraction

Cetacean <i>Morbillivirus</i>: Current knowledge and future directions

We review the molecular and epidemiological characteristics of cetacean morbillivirus (CeMV) and the diagnosis and pathogenesis of associated disease, with six different strains detected in cetaceans worldwide. CeMV has caused epidemics with high mortality in odontocetes in Europe, the USA and Australia. It represents a distinct species within the Morbillivirus genus. Although most CeMV strains are phylogenetically closely related, recent data indicate that morbilliviruses recovered from Indo-Pacific bottlenose dolphins (Tursiops aduncus), from Western Australia, and a Guiana dolphin (Sotalia guianensis), from Brazil, are divergent. The signaling lymphocyte activation molecule (SLAM) cell receptor for CeMV has been characterized in cetaceans. It shares higher amino acid identity with the ruminant SLAM than with the receptors of carnivores or humans, reflecting the evolutionary history of these mammalian taxa. In Delphinidae, three amino acid substitutions may result in a higher affinity for the virus. Infection is diagnosed by histology, immunohistochemistry, virus isolation, RT-PCR, and serology. Classical CeMV-associated lesions include bronchointerstitial pneumonia, encephalitis, syncytia, and lymphoid depletion associated with immunosuppression. Cetaceans that survive the acute disease may develop fatal secondary infections and chronic encephalitis. Endemically infected, gregarious odontocetes probably serve as reservoirs and vectors. Transmission likely occurs through the inhalation of aerosolized virus but mother to fetus transmission was also reported

Modelling micropollutant cycle in Lake Como in a winter scenario: Implications for water use and reuse, ecosystem services, and the EU zero pollution action plan

The fate and effects of 42 pharmaceuticals was studied in Lake Como (Italy), in wastewater treatment plants delivering water to the lake, in two rivers and in potable water obtained from lake water. Lake Como is one of the deepest and largest lakes in Northern Italy, serving important ecosystem services (i.e., drinking water, recreational, industrial, irrigation uses), some of which are currently at risk giving the current water scarcity and climate change scenarios. The highest concentrations measured in lake water were those of diclofenac, followed by carbamazepine, its metabolite, and clarithromycin. The data measured allowed to calibrate and run a fugacity-based lake model, which showed that the most important chemical load generally comes from the advective water from the north of the lake, rather than from the direct wastewater treatment plant (WWTP) discharges. This indicates that only an important reduction of chemical discharge (reduced use or extensive treatment) at a drainage basin level could significantly reduce concentrations in water. This has strong implications on how to implement the EU zero pollution action plan to significantly improve water ecosystem and human health protection