279 research outputs found
Repetition and difference: Lefebvre, Le Corbusier and modernity's (im)moral landscape: a commentary
This article engages with the relationship between social theory, architectural theory and material culture. The article is a reply to an article in a previous volume of the journal in question (Smith, M. (2001) ‘Repetition and difference: Lefebvre, Le Corbusier and modernity’s (im)moral landscape’, Ethics, Place and Environment, 4(1), 31-34) and, consequently, is also a direct engagement with another academic's scholarship. It represents a critique of their work as well as a recasting of their ideas, arguing that the matter in question went beyond interpretative issues to a direct critique of another author's scholarship on both Le Corbusier and Lefebvre. A reply to my article from the author of the original article was carried in a later issue of the journal (Smith, M. (2002) ‘Ethical Difference(s): a Response to Maycroft on Le Corbusier and Lefebvre’, Ethics, Place and Environment, 5(3), 260-269)
Drop on demand sample delivery for studying biocatalysts in action at X ray free electron lasers
A critical evaluation of predictive models for rooted soil strength with application to predicting the seismic deformation of rooted slopes
This paper presents a comparative study of three different classes of model for estimating the reinforcing effect of plant roots in soil, namely (i) fibre pull-out model, (ii) fibre break models (including Wu and Waldron’s Model (WWM) and the Fibre Bundle Model (FBM)) and (iii) beam bending or p-y models (specifically Beam on a Non-linear Winkler-Foundation (BNWF) models). Firstly, the prediction model of root reinforcement based on pull-out being the dominant mechanism for different potential slip plane depths was proposed. The resulting root reinforcement calculated were then compared with those derived from the other two types of models. The estimated rooted soil strength distributions were then incorporated within a fully dynamic, plane-strain continuum finite element model to assess the consequences of the selection of rooted soil strength model on the global seismic stability of a vegetated slope (assessed via accumulated slip during earthquake shaking). For the particular case considered in this paper (no roots were observed to have broken after shearing), root cohesion predicted by the pull-out model is much closer to that the BNWF model, but is largely over-predicted by the family of fibre break models. In terms of the effects on the stability of vegetated slopes, there exists a threshold value beyond which the position of the critical slip plane would bypass the rooted zones, rather than passing through them. Further increase of root cohesion beyond this value has minimal effect on the global slope behaviour. This implies that significantly over-predicted root cohesion from fibre break models when used to model roots with non-negligible bending stiffness may still provide a reasonable prediction of overall behaviour, so long as the critical failure mechanism is already bypassing the root-reinforced zones. © 2019, The Author(s)
Centrifuge modelling of the effects of root geometry on transpiration-induced suction and stability of vegetated slopes
Shallow landslides (i.e., 1 - 2 m depth) on both man-made and natural slopes are of major concern worldwide that has led to huge amount of socio-economical losses. The use of vegetation has been considered as an environmentally friendly means of stabilising slopes. Existing studies have focused on the use of plant roots with different geometries to mechanically stabilise soil slopes, but there are little data available on the contribution of transpiration-induced suction to slope stability. This study was designed to quantify both the hydrological and mechanical effects of root geometry on the stability of shallow slopes. Centrifuge tests were conducted to measure soil suction in slope models supported by newly-developed artificial roots. These artificial roots exhibit three different representative geometries (i.e., tap, heart and plate) and could simulate the effects of transpiration. The measured suction was then back-analysed through a series of finite element seepage-stability analyses to determine the factor of safety (FOS). It is revealed that after a rainfall event with a return period of 1000 years, the slope supported by heart-shaped roots retained the highest suction of 3 kPa within the root depth and thus this type of root provided the greatest stabilisation effects. The FOS of the slope supported by the heart-shaped roots, through both mechanical reinforcement and transpiration-induced suction, is 16% and 28% higher than that supported by the tap- and plate-shaped roots, respectively
Small-scale modelling of plant root systems using 3D printing, with applications to investigate the role of vegetation on earthquake-induced landslides
Vegetation has been previously proposed as a method for protecting artificial and natural slopes against shallow landslides (e.g. as may be triggered by an earthquake); however, previous research has concentrated on individual root soil interaction during shear deformation rather than the global slope behaviour due to the extreme expense and difficulty involved in conducting full-scale field tests. Geotechnical centrifuge modelling offers an opportunity to investigate in detail the engineering performance of vegetated slopes, but its application has been restricted due to the lack of availability of suitable root analogues that can repeatably replicate appropriate mechanical properties (stiffness and strength) and realistic 3-D geometry. This study employed 3-D printing to develop a representative and repeatable 1:10 scale model of a tree root cluster representing roots up to 1.5 m deep at prototype scale) that can be used within a geotechnical centrifuge to investigate the response of a vegetated slope subject to earthquake ground motion. The printed Acrylonitrile Butadiene Styrene (ABS) plastic root model was identified to be highly representative of the geometry and mechanical behaviour (stiffness and strength) of real woody root systems. A programme of large direct shear tests was also performed to evaluate the additional strength provided by the root analogues within soil that is slipping and investigate the influence of various characteristics (including root area ratio, soil confining effective stress and root morphology) on this reinforcing effect. Our results show that root reinforcement is not only a function of root mechanical properties, but also depends on factors including surrounding effective confining stress (resulting in depth dependency even for the same RAR), depth of the slip plane and root morphology. When subject to shear loading in soil, the tap root appeared to structurally transfer load within the root system, including to smaller and deeper roots which subsequently broke or were pulled out. Finally, the root analogues were added to model slopes subjected toearthquake ground motion in the centrifuge, where it was revealed that vegetation can substantially reduce earthquake-induced slope deformation in the soil conditions tested (76% reduction on crest permanent settlement during slippage). Both the realistic 3-D geometry and highly simplified root morphologies, as characterised mechanically by the shear tests, were tested in the centrifuge which, despite exhibiting very different levels of additional strength in the shear tests, resulted in very similar responses of the slopes. This suggests that once a certain minimum level of reinforcement has been reached which will alter the deformation mechanism within the slope, further increases of root contribution (e.g. due to differences in root morphology) do not have a large further effect on improving slope stability.<br/
Hybridization dynamics between sympatric species of trout: loss of reproductive isolation
Although reinforcement should enhance reproductive barriers in sympatric species, sympatric trout species do hybridize. Using mitochondrial and nuclear species markers, we investigated hybridization directionality, hybrid mating biases, and selection against hybrids in 13 sympatric cutthroat and rainbow trout populations on Vancouver Island, Canada. Approximately 50% of the genotyped fish were hybrid (F1 or higher-order) and populations ranged from very recent (all F1 hybrids) to extremely advanced higher-order hybridization. Overall, interbreeding was reciprocal, although some populations showed directional hybridization. Pronounced cytonuclear disequilibrium in post-F1 hybrids indicated a remarkable mating bias not previously reported, which is most likely because of behavioural reproductive preferences. Selection against hybrids was observed in only two populations, indicative of extrinsic selection. Two populations were ‘hybrid swarms’, with a complete loss of reproductive isolation. The complex hybridization dynamics in this system represent a valuable natural experiment of the genetic and evolutionary implications of recent and on-going interspecific hybridization
Genetic differentiation among sympatric migratory and resident life history forms of rainbow trout in British Columbia
Rainbow trout Oncorhynchus mykiss in coastal areas of North America occur as two divergent migratory types: steelhead that migrate to salt water and return to their natal river to spawn and resident rainbow trout that either do not migrate or migrate locally within the freshwater system. We extracted DNA from sympatrically occurring steelhead and rainbow trout collected from five major drainages in British Columbia. We used three types of genetic markers to test for differentiation within the sympatric population: microsatellite DNA markers, gene intron polymorphisms (restriction fragment length polymorphisms [RFLPs] and fragment size polymorphisms), and major histocompatibility complex (MHC) exon polymorphisms (RFLPs). The migratory and resident forms were genetically differentiated in only one sympatric population based on microsatellite data, but no significant differentiation was found using the combined gene locus marker data. Overall, most of the observed allele variation at all marker types was attributable to among-drainage variance, while migratory life history contributed a nonsignificant component based on hierarchical analysis of molecular variance. Interestingly, the migratory and resident forms were genetically differentiated in two different populations based on the gonadotropin and p53 intron polymorphisms, while no significant genetic differentiation was found between the two migratory types at any of the MHC exon or growth hormone intron-length polymorphisms. Furthermore, the neighbor-joining cluster dendrogram based on the microsatellite markers reflected geographic patterns, while the neighborjoining dendrogram using gene intron and MHC markers did not. Our data indicate that the migratory and resident life histories have probably evolved independently in different drainages in British Columbia. The disagreement between the results of the analyses using the microsatellite and gene locus markers probably reflects differences in selection, mutation, or both, acting at these two types of marker loci
Modeling the interactions between river morphodynamics and riparian vegetation
The study of river-riparian vegetation interactions is an important and intriguing research field in geophysics. Vegetation is an active element of the ecological dynamics of a floodplain which interacts with the fluvial processes and affects the flow field, sediment transport, and the morphology of the river. In turn, the river provides water, sediments, nutrients, and seeds to the nearby riparian vegetation, depending on the hydrological, hydraulic, and geomorphological characteristic of the stream. In the past, the study of this complex theme was approached in two different ways. On the one hand, the subject was faced from a mainly qualitative point of view by ecologists and biogeographers. Riparian vegetation dynamics and its spatial patterns have been described and demonstrated in detail, and the key role of several fluvial processes has been shown, but no mathematical models have been proposed. On the other hand, the quantitative approach to fluvial processes, which is typical of engineers, has led to the development of several morphodynamic models. However, the biological aspect has usually been neglected, and vegetation has only been considered as a static element. In recent years, different scientific communities (ranging from ecologists to biogeographers and from geomorphologists to hydrologists and fluvial engineers) have begun to collaborate and have proposed both semiquantitative and quantitative models of river-vegetation interconnections. These models demonstrate the importance of linking fluvial morphodynamics and riparian vegetation dynamics to understand the key processes that regulate a riparian environment in order to foresee the impact of anthropogenic actions and to carefully manage and rehabilitate riparian areas. In the first part of this work, we review the main interactions between rivers and riparian vegetation, and their possible modeling. In the second part, we discuss the semiquantitative and quantitative models which have been proposed to date, considering both multi- and single-thread river
PCR-RFLP markers detect 29 mitochondrial haplotypes in Pacific lamprey (Entosphenus tridentatus)
We developed five polymerase chain reaction-based markers that detect variation in the mitochondrial genome of the Pacific lamprey, Entosphenus tridentatus, across most of its range. Two gene fragments (ND2 and ND5) were amplified and digested with three and two restriction enzymes, respectively, detecting sequence variation at 18 sites (ND2 = 13; ND5 = 5) and yielding 29 composite haplotypes among 1246 lampreys. These sequencebased markers will be useful in a range of phylogeographical and population genetic studies
Contrasting physiological responses between invasive sea lamprey and non-target bluegill in response to acute lampricide exposure
Control of invasive sea lamprey (Petromyzon marinus) in the Laurentian Great Lakes of North America uses lampricides, which consist of 3-trifluoromethyl-4-nitrophenol (TFM) and niclosamide. Lampricides are thought to inhibit aerobic energy synthesis, with TFM having a relatively greater selective action against lampreys. While the toxicity and physiological effects of TFM are known, the impacts associated with exposure to niclosamide and TFM:niclosamide mixtures are poorly characterized in fishes. Therefore, focusing on energy metabolism, we quantified the physiological responses of larval sea lamprey and bluegill (Lepomis macrochirus), a non-target, native species. Exposures consisted of each lampricide alone (TFM at the species-specific 24 h LC10; niclosamide at 1.5% of the mixture's TFM concentration) or a mixture of the two (larval sea lamprey at TFM 24 h LC10 + 1.5% niclosamide; bluegill at sea lamprey's TFM 24 h LC99.9 + 1.5% niclosamide) for 24 h. Tissues (brain, skeletal muscle, and liver) were sampled at 6, 12, and 24 h of exposure and assayed for concentrations of ATP, phosphocreatine, glycogen, lactate, and glucose and tissue lampricide levels. In larval sea lamprey, TFM had little effect on brain and skeletal muscle, but niclosamide resulted in a depletion of high energy substrates in both tissues. Mixture-exposed lamprey showed depletion of high energy substrates, accumulation of lactate, and high mortality rates. Bluegill were largely unaffected by toxicant exposures. However, bluegill liver showed lower glycogen and lactate under all three toxicant exposures suggesting increased metabolic turnover. Bluegill also had lower concentrations of TFM and niclosamide in their tissues when compared to lamprey. Our results indicate that lampricide toxicity in sea lamprey larvae is mediated through a depletion of high energy substrates because of impaired aerobic ATP synthesis. We also confirmed that non-target bluegill showed high tolerance to lampricide exposure, an effect potentially mediated through a high detoxification capacity relative to lampreys.Fisheries and Oceans Canada||Great Lakes Fishery Commission (Grant #2018_JEF_54072)||University of Manitoba||Natural Sciences and Engineering Research Council of Canada(#05479
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