Effect of vernalization period and bulb size on bolting of onion cultivar “Texas Early Grano” grown under Gezira conditions, Sudan

     Texas Early Grano onion cultivar is very popular in Sudan and is used mainly as a salad vegetable. Unfortunately, this cultivar does not flower and produce seeds under Sudan conditions and seeds should be imported from abroad. Attempts to produce seeds of this cultivar under Gezira conditions have failed. Therefore, the objective of this research was to study the effects of bulb size and vernalization on the induction of flowering of Texas Early Grano onion cultivar. Experiments were conducted at the research farm of the Faculty of Agricultural Sciences, University of Gezira, Wad Medani, Sudan, during the  winter seasons of 2008/09 and 2009/10.   Treatments consisted of vernalization periods ranging from 45 to 180 days, storage temperatures of 4 to 50C, and bulb size which was small and medium. Treatments were arranged in a  randomized complete block design with three replicates. Results indicated that vernalization duration significantly affected emergence percentage. As vernalization duration increased, emergence percentage increased in both seasons. Bulb size had no significant effects on emergence percentage. Vernalization temperatures of 4 to 50C for 90 days or more was the key factor for the induction of bolting of Texas Early Grano cultivar. To obtain the highest bolting percentage and seed yield, a combination of venrnalization at 4 to 50C for 180 days and medium-sized bulbs should be recommended.       يعتبر صنف البصل "تكساس إيرلى جرانو" من الأصناف المعروفة في السودان التي تستخدم كأحد الخضر الرئيسية في طبق السلطة. محاولة إنتاج بذور هذا الصنف محلياً لم تعط نتائج إيجابية نسبة لعدم توفر درجات الحرارة المنخفضة اللازمة لتحفيز الإزهار تحت ظروف ولاية الجزيرة. لذا هدفت هذه الدراسة إلى تحفيز الإزهار لإنتاج البذور من هذا الصنف باستخدام ظاهرة الإرتباع (التخزين المبرد) لأحجام مختلفة من الأبصال. أجريت هذه الدراسة بمزرعة كلية العلوم الزراعية، جامعة الجزيرة، واد مدني، السودان خلال موسمي 2008/09 و 2009/10م. شملت التجارب فترات تخزين مبرد تراوحت ما بين 45- 180 يوما في درجات حرارة تراوحت ما بين 4 إلي 50 م ونوعين من حجم الأبصال (صغير ومتوسط).  استخدم تصميم القطاعات العشوائية الكاملة بثلاث مكررات. أشارت النتائج إلى أن التخزين المبرد في درجات الحرارة 4 - 5°م هو العامل الرئيسي المحفز للإزهار في صنف البصل "تكساس إيرلي جرانو". التخزين المبرد أظهر تاثيراً معنوياً في نسبة الانبات، فكلما زادت فترة التخزين المبرد زادت نسبة الانبات في كلا الموسمين، بينما لم يؤثر حجم الأبصال في نسبة الانبات. تم الحصول على أعلى نسبة للإزهار وإنتاجية البذور بزراعة الأبصال متوسطة الحجم والمخزنة في درجة حرارة 4 - 5°م لفترة 180 يوما. لذلك يوصى بتخزين الأبصال متوسطة الحجم في درجات حرارة تتراوح بين 4 - 5°م لفترة 180 يوما أو اكثر للحصول على بذور صنف البصل "تكساس إيرلي جرانو" تحت ظروف ولاية الجزيرة

Chapter 11 - Agriculture, forestry and other land use (AFOLU)

Agriculture, Forestry, and Other Land Use (AFOLU) plays a central role for food security and sustainable development. Plants take up carbon dioxide (CO2) from the atmosphere and nitrogen (N) from the soil when they grow, re-distributing it among different pools, including above and below-ground living biomass, dead residues, and soil organic matter. The CO2 and other non-CO2 greenhouse gases (GHG), largely methane (CH4) and nitrous oxide (N2O), are in turn released to the atmosphere by plant respiration, by decomposition of dead plant biomass and soil organic matter, and by combustion. Anthropogenic land-use activities (e.g., management of croplands, forests, grasslands, wetlands), and changes in land use / cover (e.g., conversion of forest lands and grasslands to cropland and pasture, afforestation) cause changes superimposed on these natural fluxes. AFOLU activities lead to both sources of CO2 (e.g., deforestation, peatland drainage) and sinks of CO2 (e.g., afforestation, management for soil carbon sequestration), and to non-CO2 emissions primarily from agriculture (e.g., CH4 from livestock and rice cultivation, N2O from manure storage and agricultural soils and biomass burning. The main mitigation options within AFOLU involve one or more of three strategies: reduction / prevention of emissions to the atmosphere by conserving existing carbon pools in soils or vegetation that would otherwise be lost or by reducing emissions of CH4 and N2O; sequestration - enhancing the uptake of carbon in terrestrial reservoirs, and thereby removing CO2 from the atmosphere; and reducing CO2 emissions by substitution of biological products for fossil fuels or energy-intensive products. Demand-side options (e.g., by lifestyle changes, reducing losses and wastes of food, changes in human diet, changes in wood consumption), though known to be difficult to implement, may also play a role. Land is the critical resource for the AFOLU sector and it provides food and fodder to feed the Earth's population of ~7 billion, and fibre and fuel for a variety of purposes. It provides livelihoods for billions of people worldwide. It is finite and provides a multitude of goods and ecosystem services that are fundamental to human well-being. Human economies and quality of life are directly dependent on the services and the resources provided by land. Figure 11.1 shows the many provisioning, regulating, cultural and supporting services provided by land, of which climate regulation is just one. Implementing mitigation options in the AFOLU sector may potentially affect other services provided by land in positive or negative ways. In the Intergovernmental Panel on Climate Change (IPCC) Second Assessment Report (SAR) and in the IPCC Fourth Assessment Report (AR4), agricultural and forestry mitigation were dealt with in separate chapters. In the IPCC Third Assessment Report (TAR), there were no separate sectoral chapters on either agriculture or forestry. In the IPCC Fifth Assessment Report (AR5), for the first time, the vast majority of the terrestrial land surface, comprising agriculture, forestry and other land use (AFOLU), is considered together in a single chapter, though settlements (which are important, with urban areas forecasted to triple in size from 2000 global extent by 2030), are dealt with in Chapter 12. This approach ensures that all land-based mitigation options can be considered together; it minimizes the risk of double counting or inconsistent treatment (e.g., different assumptions about available land) between different land categories, and allows the consideration of systemic feedbacks between mitigation options related to the land surface. Considering AFOLU in a single chapter allows phenomena common across land-use types, such as competition for land and water, co-benefits, adverse side-effects and interactions between mitigation and adaptation to be considered consistently. The complex nature of land presents a unique range of barriers and opportunities, and policies to promote mitigation in the AFOLU sector need to take account of this complexity. In this chapter, we consider the competing uses of land for mitigation and for providing other services. Unlike the chapters on agriculture and forestry in AR4, impacts of sourcing bioenergy from the AFOLU sector are considered explicitly in a dedicated appendix. Also new to this assessment is the explicit consideration of food / dietary demand-side options for GHG mitigation in the AFOLU sector, and some consideration of freshwater fisheries and aquaculture, which may compete with the agriculture and forestry sectors, mainly through their requirements for land and / or water, and indirectly, by providing fish and other products to the same markets as animal husbandry. This chapter deals with AFOLU in an integrated way with respect to the underlying scenario projections of population growth, economic growth, dietary change, land-use change (LUC), and cost of mitigation. We draw evidence from both "bottom-up" studies that estimate mitigation potentials at small scales or for individual options or technologies and then scale up, and multi-sectoral "top-down" studies that consider AFOLU as just one component of a total multi-sector system response. In this chapter, we provide updates on emissions trends and changes in drivers and pressures in the AFOLU sector, describe the practices available in the AFOLU sector, and provide refined estimates of mitigation costs and potentials for the AFOLU sector, by synthesising studies that have become available since AR4. We conclude the chapter by identifying gaps in knowledge and data, providing a selection of Frequently Asked Questions, and presenting an Appendix on bioenergy to update the IPCC Special Report on Renewable Energy Sources and Climate Change Mitigation (SRREN)

Ultrasound- guided fine needle aspiration cytology and cell block in the diagnosis of focal liver lesions at Khartoum Hospital, Sudan

Background: The appropriate clinical management of various hepatic lesions depends on accurate diagnosis. Fine needle aspiration and cell block have gained popularity because they are convenient, minimally invasive, quick and have good performance profiles.Objective: To investigate the cytomorphological features of distinctive non-neoplastic and neoplastic lesions of the liver and to evaluate the sensitivity, specificity and diagnostic accuracy of ultrasound- guided (USG) fine needle aspiration cytology (FNAC) and cell block in the diagnosis ofliver diseases at Khartoum Teaching Hospital.Method: A cross-sectional study was conducted at Khartoum Teaching Hospital, Sudan during the period of November 2008 to October 2011. One hundred and five cytological materials were collected from patients with focal liver lesions who referred after initial clinical and radiologicalassessment for ultrasound–guided fine needle aspiration cytology (USG -FNAC).Results: The age of the patients ranged from 5- 60 years and 64 (61%) of these patients were males. Out of 105 investigated samples 76 (72.4%) and 41(39.0%) were malignant by cytology and cell block respectively. Out of these 105 samples, 71 were investigated by both cytology and cell blocks and were included in the final analyses of the FNAC in comparison to cell block. The sensitivity, specificity, positive predictive value and negative predictive value of the cytology were 100% (93.0 – 100%), 63.3% (45.2 – 79.0%), 78.8% (66.2 – 88.3%) and 0 (0 – 14.6%), respectively.Conclusion: Thus, USG -FNAC is a sensitive but not specific method in differentiating the benign and malignant focal liver lesions. Differentiation between primary liver malignant lesions and metastatic lesion needs adjunct techniqueKey words: neoplastic lesion, non-neoplastic lesio

Primary Tuberculosis of the Maxillary Sinus: A case report

Tuberculosis is up-to-date a leading cause of human suffering and loss of life. Although tuberculosis is a systemic disease, primary tuberculosis of the paranasal sinuses is rare. It should be kept in mind in any undiagnosed or atypical clinical infection or inflammation in this region. We present here a case of primary paranasal sinuses tuberculosis in an elderly Sudanese woman. Although Sudan is one of the highest three countries in the incidence of tuberculosis, this is the first case to be reported in Suda

HR-EBSD analysis of in situ stable crack growth at the micron scale

Understanding the local fracture resistance of microstructural features. such as brittle inclusions, coatings, and interfaces at the microscale under complex loading conditions is critical for microstructure-informed design of materials. In this study, a novel approach has been formulated to decompose the J-integral evaluation of the elastic energy release rate to the three-dimensional stress intensity factors directly from experimental measurements of the elastic deformation gradient tensors of the crack field by in situ high (angular) resolution electron backscatter diffraction (HR-EBSD). An exemplar study is presented of a quasi-static crack, inclined to the observed surface, propagating on low index {hkl} planes in a (001) single crystal silicon wafer

Occurrence and Antimicrobial Susceptibility Profiles of Escherichia coli O157 Isolated from Beef and Poultry Meat in Khartoum State, Sudan

Escherichia coli O157 is a major food-borne pathogen transmitted by consuming contaminated food and water. This study was conducted between January and April 2021 on 400 samples of beef and poultry meat to determine the occurrence and antimicrobial susceptibility profiles of E. coli O157 in Khartoum State, according to the methods of ISO 16654 and Clinical and Laboratory Standards Institute (CLSI). The overall presence rate was found to be 1.7% (7/400). Two isolates were recovered from 200 beef meat samples (1%) and five isolates from 200 poultry meat samples (2.5%). All isolates were sensitive to ciprofloxacin and trimethoprim-sulfamethoxazole, but all were resistant to ampicillin (100%). Six isolates (85.7%) showed resistance to tetracycline, and five (71.4%) to gentamicin. Three isolates (42.8%) showed intermediate sensitivity to ceftazidime, and others (42.8%) were resistant to it. Six isolates (85.5%) showed multiple drug resistance (MDR). This study revealed the presence of resistant isolates in beef and poultry meat. To reduce the incidence of foodborne E. coli O157 species and antimicrobial resistance in Khartoum State, scientists and public health researchers need to know that E. coli O157 is present in beef and poultry meat in the region

Laparoscopy in management of appendicitis in high-, middle-, and low-income countries: a multicenter, prospective, cohort study.

BACKGROUND: Appendicitis is the most common abdominal surgical emergency worldwide. Differences between high- and low-income settings in the availability of laparoscopic appendectomy, alternative management choices, and outcomes are poorly described. The aim was to identify variation in surgical management and outcomes of appendicitis within low-, middle-, and high-Human Development Index (HDI) countries worldwide. METHODS: This is a multicenter, international prospective cohort study. Consecutive sampling of patients undergoing emergency appendectomy over 6 months was conducted. Follow-up lasted 30 days. RESULTS: 4546 patients from 52 countries underwent appendectomy (2499 high-, 1540 middle-, and 507 low-HDI groups). Surgical site infection (SSI) rates were higher in low-HDI (OR 2.57, 95% CI 1.33-4.99, p = 0.005) but not middle-HDI countries (OR 1.38, 95% CI 0.76-2.52, p = 0.291), compared with high-HDI countries after adjustment. A laparoscopic approach was common in high-HDI countries (1693/2499, 67.7%), but infrequent in low-HDI (41/507, 8.1%) and middle-HDI (132/1540, 8.6%) groups. After accounting for case-mix, laparoscopy was still associated with fewer overall complications (OR 0.55, 95% CI 0.42-0.71, p < 0.001) and SSIs (OR 0.22, 95% CI 0.14-0.33, p < 0.001). In propensity-score matched groups within low-/middle-HDI countries, laparoscopy was still associated with fewer overall complications (OR 0.23 95% CI 0.11-0.44) and SSI (OR 0.21 95% CI 0.09-0.45). CONCLUSION: A laparoscopic approach is associated with better outcomes and availability appears to differ by country HDI. Despite the profound clinical, operational, and financial barriers to its widespread introduction, laparoscopy could significantly improve outcomes for patients in low-resource environments. TRIAL REGISTRATION: NCT02179112

Association of Cytokeratin and Vimentin Protein in the Genesis of Transitional Cell Carcinoma of Urinary Bladder Patients

The aim of study was to examine the localization and distribution of cytokeratin (CK) and vimentin protein and their association with clinical outcome of the TCC patients. Expression pattern of cytokeratin and vimentin was evaluated by immunohistochemistry in TCC cases and inflammatory lesions. Cytoplasmic expression of CK was noticed in 52.17% of TCC cases and its expression was not observed in inflammatory lesions of bladder specimens. Vimentin showed expression in 69.00% cases of TCC. Significant differences were noticed in expression pattern of CK and vimentin in inflammatory lesion and Transitional Cell Carcinoma cases. Vimentin expression increased with the grade of TCC and this difference was statistically significant whereas expression of CK decreased according to the grade of TCC. Furthermore, it was also observed that expression pattern of vimentin was high in ≥55 years as compared to &lt;55 age group patients and these differences were significant in men as compared to women. Expression pattern of CK did not show any significant relation with age and gender. Therefore, it can be concluded that cytokeratin and vimentin will be helpful markers in the early diagnosis of Transitional Cell Carcinoma/bladder carcinoma.</jats:p

Application of a multiplex CRISPR/Cas9 strategy for elimination of selection markers from transgenic plants

Selectable marker genes (SMGs) are essential for identifying transgenic plants but raise concerns regarding biosafety, regulatory compliance, and public acceptance. In this study, we used a CRISPR/Cas9-based strategy to eliminate the SMG from transgenic tobacco plants. Leaf discs from plants carrying DsRED (SMG) and aminoglycoside phosphotransferase (gene of interest, GOI) were re-transformed with a CRISPR vector containing four gRNAs designed to target both flanking regions of the SMG cassette. Approximately 20% of the regenerated shoots exhibited loss of red fluorescence, and PCR and sequencing analyses confirmed that about half of these carried a smaller amplicon, indicating a successful SMG excision efficiency of around 10%. Mutation analysis further revealed the presence of small indels at gRNA target sites, in addition to the deletion of SMG cassette. Quantitative real-time PCR (qPCR) analysis confirmed the absence of DsRED expression in SMG-deleted lines, while the Cas9 and GOI remained actively expressed. The SMG-free plants displayed normal growth, flowering, and seed production, indicating CRISPR marker excision had no adverse effects on plant development and fertility. In addition, Cas9-free, marker-free transgenic plants were recovered through segregation in T1 generation. This approach is adaptable to various transgenic plant species and provides a practical solution for generating marker-free transgenic crops, thereby enhancing their acceptance and commercialization

Ticarcillin degradation product thiophene acetic acid is a novel auxin analog that promotes organogenesis in tomato

Efficient regeneration of transgenic plants from explants after transformation is one of the crucial steps in developing genetically modified plants with desirable traits. Identification of novel plant growth regulators and developmental regulators will assist to enhance organogenesis in culture. In this study, we observed enhanced shoot regeneration from tomato cotyledon explants in culture media containing timentin, an antibiotic frequently used to prevent Agrobacterium overgrowth after transformation. Comparative transcriptome analysis of explants grown in the presence and absence of timentin revealed several genes previously reported to play important roles in plant growth and development, including Auxin Response Factors (ARFs), GRF Interacting Factors (GIFs), Flowering Locus T (SP5G), Small auxin up-regulated RNAs (SAUR) etc. Some of the differentially expressed genes were validated by quantitative real-time PCR. We showed that ticarcillin, the main component of timentin, degrades into thiophene acetic acid (TAA) over time. TAA was detected in plant tissue grown in media containing timentin. Our results showed that TAA is indeed a plant growth regulator that promotes root organogenesis from tomato cotyledons in a manner similar to the well-known auxins, indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA). In combination with the cytokinin 6-benzylaminopurine (BAP), TAA was shown to promote shoot organogenesis from tomato cotyledon in a concentration-dependent manner. To the best of our knowledge, the present study reports for the first time demonstrating the function of TAA as a growth regulator in a plant species. Our work will pave the way for future studies involving different combinations of TAA with other plant hormones which may play an important role in in vitro organogenesis of recalcitrant species. Moreover, the differentially expressed genes and long noncoding RNAs identified in our transcriptome studies may serve as contender genes for studying molecular mechanisms of shoot organogenesis