Waddlia chondrophila induces systemic infection, organ pathology, and elicits Th1-associated humoral immunity in a murine model of genital infection.

Waddlia chondrophila is a known bovine abortigenic Chlamydia-related bacterium that has been associated with adverse pregnancy outcomes in human. However, there is a lack of knowledge regarding how W. chondrophila infection spreads, its ability to elicit an immune response and induce pathology. A murine model of genital infection was developed to investigate the pathogenicity and immune response associated with a W. chondrophila infection. Genital inoculation of the bacterial agent resulted in a dose-dependent infection that spread to lumbar lymph nodes and successively to spleen and liver. Bacterial-induced pathology peaked on day 14, characterized by leukocyte infiltration (uterine horn, liver, and spleen), necrosis (liver) and extramedullary hematopoiesis (spleen). Immunohistochemistry demonstrated the presence of a large number of W. chondrophila in the spleen on day 14. Robust IgG titers were detected by day 14 and remained high until day 52. IgG isotypes consisted of high IgG2a, moderate IgG3 and no detectable IgG1, indicating a Th1-associated immune response. This study provides the first evidence that W. chondrophila genital infection is capable of inducing a systemic infection that spreads to major organs, induces uterus, spleen, and liver pathology and elicits a Th1-skewed humoral response. This new animal model will help our understanding of the mechanisms related to intracellular bacteria-induced miscarriages, the most frequent complication of pregnancy that affects one in four women

Resonances Width in Crossed Electric and Magnetic Fields

We study the spectral properties of a charged particle confined to a two-dimensional plane and submitted to homogeneous magnetic and electric fields and an impurity potential. We use the method of complex translations to prove that the life-times of resonances induced by the presence of electric field are at least Gaussian long as the electric field tends to zero.Comment: 3 figure

A centrifugally controlled circuit in the avian retina and its possible role in visual attention switching.

The isthmo-optic nucleus (ION) is the main source of efferents to the retina in birds. Isthmo-optic neurons project in topographical order on amacrine cells in the ventral parts of the retina, and a subclass of these known as proprioretinal neurons project onto the dorsal retina. We propose that, through the intermediary of the amacrine target cells, activity in the isthmo-optic pathway excites ganglion cells locally in the ventral retina but inhibits those in dorsal regions. This circuit would thereby mediate centrifugally controlled switches in attention between the dorsal retina, involved in feeding, and the more ventral parts, involved in scanning for predators. This hypothesis accounts for a wide range of disparate data from behavior, comparative anatomy, endocrinology, hodology, and neurophysiology

Contactless Gait Assessment in Home-like Environments

Gait analysis is an important part of assessments for a variety of health conditions, specifically neurodegenerative diseases. Currently, most methods for gait assessment are based on manual scoring of certain tasks or restrictive technologies. We present an unobtrusive sensor system based on light detection and ranging sensor technology for use in home-like environments. In our evaluation, we compared six different gait parameters, based on recordings from 25 different people performing eight different walks each, resulting in 200 unique measurements. We compared the proposed sensor system against two state-of-the art technologies, a pressure mat and a set of inertial measurement unit sensors. In addition to test usability and long-term measurement, multi-hour recordings were conducted. Our evaluation showed very high correlation (r>0.95) with the gold standards across all assessed gait parameters except for cycle time (r=0.91). Similarly, the coefficient of determination was high (R2>0.9) for all gait parameters except cycle time. The highest correlation was achieved for stride length and velocity (r≥0.98,R2≥0.95). Furthermore, the multi-hour recordings did not show the systematic drift of measurements over time. Overall, the unobtrusive gait measurement system allows for contactless, highly accurate long- and short-term assessments of gait in home-like environments

Phonon heat capacity and self-heating normal domains in NbTiN nanostrips

Self-heating normal domains in thin superconducting NbTiN nanostrips were characterized via steady-state hysteretic current-voltage characteristics measured at different substrate temperatures. The temperature dependence and the magnitude of the current, which sustains a domain in equilibrium at different voltages, can only be explained with a phonon heat capacity noticeably less than expected for 3-d Debye phonons. This reduced heat capacity coincides with the value obtained earlier from magnetoconductance and photoresponse studies of the same films. The rate of heat flow from electrons at a temperature Te to phonons in the substrate at a temperature TB is proportional to (T_e^p - T_B^p) with the exponent p~3, which differs from the exponents for heat flows mediated by the electron-phonon interaction or by escaping of 3-d Debye phonons via the film/substrate interface. We attribute both findings to the effect of the mean grain size on the phonon spectrum of thin granular NbTiN films. Our findings are significant for understanding the thermal transport in superconducting devices exploiting thin granular films

Magnetoconductance and photoresponse properties of disordered NbTiN films

We report on the experimental study of phonon properties and electron-phonon scattering in thin superconducting NbTiN films, which are intensively exploited in various applications. Studied NbTiN films with sub-10-nm thicknesses are disordered with respect to electron transport, the Ioffe-Regel parameter of kFle=2.5–3.0 (kF is the Fermi wave vector, and le is the electron mean free path), the inelastic electron-phonon interaction, and the product qTle≪1 (qT is the wave vector of a thermal phonon). By means of magnetoconductance and photoresponse techniques, we derive the inelastic electron-phonon scattering rate 1/τe-ph and determine sound velocities and phonon heat capacities. In the temperature range from 12 to 20 K, the scattering rate varies with temperature as 1/τe-ph∝T3.45±0.05; its value extrapolated to 10 K amounts to approximately 1/16 ps. Making a comparative analysis of our films and other films used in superconducting devices, such as polycrystalline granular NbN and amorphous WSi, we find a systematic reduction of the sound velocity in all these films by about 50% compared to the corresponding bulk crystalline materials. A corresponding increase in the phonon heat capacities in all these films is, however, less than the Debye model predicts. We attribute these findings to reduced film dimensionality and film morphology

A two-dimensional optomechanical crystal for quantum transduction

Integrated optomechanical systems are one of the leading platforms for manipulating, sensing, and distributing quantum information. The temperature increase due to residual optical absorption sets the ultimate limit on performance for these applications. In this work, we demonstrate a two-dimensional optomechanical crystal geometry, named \textbf{b-dagger}, that alleviates this problem through increased thermal anchoring to the surrounding material. Our mechanical mode operates at 7.4 GHz, well within the operation range of standard cryogenic microwave hardware and piezoelectric transducers. The enhanced thermalization combined with the large optomechanical coupling rates, $g_0/2\pi \approx 880~\mathrm{kHz}$, and high optical quality factors, $Q_\text{opt} = 2.4 \times 10^5$, enables the ground-state cooling of the acoustic mode to phononic occupancies as low as $n_\text{m} = 0.35$ from an initial temperature of 3 kelvin, as well as entering the optomechanical strong-coupling regime. Finally, we perform pulsed sideband asymmetry of our devices at a temperature below 10 millikelvin and demonstrate ground-state operation ($n_\text{m} < 0.45$) for repetition rates as high as 3 MHz. Our results extend the boundaries of optomechanical system capabilities and establish a robust foundation for the next generation of microwave-to-optical transducers with entanglement rates overcoming the decoherence rates of state-of-the-art superconducting qubits.Comment: 13 pages, 4 main figure

Continuous and simultaneous multigas monitoring using a highly sensitive and selective photoacoustic sensor

A multi-gas photoacoustic sensor is reported. Simultaneous measurement of ammonia, carbon dioxide and water vapor is demonstrated at ppm level using semiconductor lasers. In-situ measurements to control the housing conditions of laboratory animals are presente