Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts

This article has been made available through the Brunel Open Access Publishing Fund.Background: Radial chromosome positioning in interphase nuclei is nonrandom and can alter according to developmental, differentiation, proliferation, or disease status. However, it is not yet clear when and how chromosome repositioning is elicited. Results: By investigating the positioning of all human chromosomes in primary fibroblasts that have left the proliferative cell cycle, we have demonstrated that in cells made quiescent by reversible growth arrest, chromosome positioning is altered considerably. We found that with the removal of serum from the culture medium, chromosome repositioning took less than 15 minutes, required energy and was inhibited by drugs affecting the polymerization of myosin and actin. We also observed that when cells became quiescent, the nuclear distribution of nuclear myosin 1ß was dramatically different from that in proliferating cells. If we suppressed the expression of nuclear myosin 1ß by using RNA-interference procedures, the movement of chromosomes after 15 minutes in low serum was inhibited. When high serum was restored to the serum-starved cultures, chromosome repositioning was evident only after 24 to 36 hours, and this coincided with a return to a proliferating distribution of nuclear myosin 1ß. Conclusions: These findings demonstrate that genome organization in interphase nuclei is altered considerably when cells leave the proliferative cell cycle and that repositioning of chromosomes relies on efficient functioning of an active nuclear motor complex that contains nuclear myosin 1ß.Brunel Open Access Publishing Fun

Transformations between HCLP and PCSP

We present a general methodology for transforming between HCLP and PCSP in both directions. HCLP and PCSP each have advantages when modelling problems, and each have advantages when implementing models and solving them. Using the work presented in this paper, the appropriate paradigm can be used for each of these steps, with a meaning-preserving transformation in between if necessary

A process algebra for synchronous concurrent constraint programming

Concurrent constraint programming is classically based on asynchronous communication via a shared store. This paper presents new version of the ask and tell primitives which features synchronicity. Our approach is based on the idea of telling new information just in the case that a concurrently running process is asking for it. An operational and an algebraic semantics are defined. The algebraic semantics is proved to be sound and complete with respect to a compositional operational semantics which is also presented in the paper

Jet Trimming

Initial state radiation, multiple interactions, and event pileup can contaminate jets and degrade event reconstruction. Here we introduce a procedure, jet trimming, designed to mitigate these sources of contamination in jets initiated by light partons. This procedure is complimentary to existing methods developed for boosted heavy particles. We find that jet trimming can achieve significant improvements in event reconstruction, especially at high energy/luminosity hadron colliders like the LHC.Comment: 20 pages, 11 figures, 3 tables - Minor changes to text/figure

The one-loop six-dimensional hexagon integral and its relation to MHV amplitudes in N=4 SYM

We provide an analytic formula for the (rescaled) one-loop scalar hexagon integral $\tilde\Phi_6$ with all external legs massless, in terms of classical polylogarithms. We show that this integral is closely connected to two integrals appearing in one- and two-loop amplitudes in planar $\\mathcal{N}=4$ super-Yang-Mills theory, $\Omega^{(1)}$ and $\Omega^{(2)}$. The derivative of $\Omega^{(2)}$ with respect to one of the conformal invariants yields $\tilde\Phi_6$, while another first-order differential operator applied to $\tilde\Phi_6$ yields $\Omega^{(1)}$. We also introduce some kinematic variables that rationalize the arguments of the polylogarithms, making it easy to verify the latter differential equation. We also give a further example of a six-dimensional integral relevant for amplitudes in $\\mathcal{N}=4$ super-Yang-Mills.Comment: 18 pages, 2 figure

Design of Marine Protected Areas on high seas and territorial waters of rockall bank

Fisheries closures are rapidly being developed to protect vulnerable marine ecosystems worldwide. Satellite monitoring of fishing vessel activity indicates that these closures can work effectively with good compliance by international fleets even in remote areas. Here we summarise how remote fisheries closures were designed to protect Lophelia pertusa habitat in a region of the NE Atlantic that straddles the EU fishing zone and the high seas. We show how scientific records, fishers' knowledge and surveillance data on fishing activity can be combined to provide a powerful tool for the design of Marine Protected Areas. © Inter-Research 2009

The Yangian origin of the Grassmannian integral

In this paper we analyse formulas which reproduce different contributions to scattering amplitudes in N=4 super Yang-Mills theory through a Grassmannian integral. Recently their Yangian invariance has been proved directly by using the explicit expression of the Yangian level-one generators. The specific cyclic structure of the form integrated over the Grassmannian enters in a crucial way in demonstrating the symmetry. Here we show that the Yangian symmetry fixes this structure uniquely.Comment: 26 pages. v2: typos corrected, published versio

Comments on gauge-invariance in cosmology

We revisit the gauge issue in cosmological perturbation theory, and highlight its relation to the notion of covariance in general relativity. We also discuss the similarities and differences of the covariant approach in perturbation theory to the Bardeen or metric approach in a non-technical fashion.Comment: 7 pages, 1 figure, revtex4; v3: minor changes, typos corrected, discussion extended; v4: typos corrected, corresponding to published versio