Primer relevamiento de marcadores de resistencia a antibióticos en Enterobacteriaceae en Cochabamba, Bolivia

Se llevó a cabo un relevamiento molecular de la resistencia a antibióticos de importancia clínica en aislamientos recuperados en Cochabamba, Bolivia. Se estudiaron los genes codificantes de β-lactamasas de espectro extendido y de resistencia a quinolonas de localización plasmídica (PMQR) en un total de 101 aislamientos de enterobacterias resistentes a oximinocefalosporinas recuperados en distintos centros de salud, durante 4 meses (2012-2013). En todos ellos se detectó la presencia de cefotaximasas, las CTX-M grupo 1 fueron las más prevalentes (88,1%). La presencia de blaOXA-1 se detectó en el 76,4% de estos aislamientos. Se observó una elevada proporción de aislamientos resistentes a quinolonas. El gen aac(6′)-Ib-cr fue el determinante PMQR más frecuentemente identificado (83%). Además, 6 aislamientos resultaron ser portadores de qnrB. Por otro lado, cabe remarcar que 7 Escherichia coli presentaron qepA1 (6) y oqxAB (1); se documenta así por primera vez la presencia de oqxAB en Bolivia. Este estudio constituye el primer relevamiento de marcadores de resistencia en aislamientos clínicos de enterobacterias en Cochabamba, Bolivia; de este modo se contribuye al conocimiento regional de la situación epidemiológica, la cual presenta un escenario similar al observado en el resto de Latinoamérica.A molecular survey was conducted in Cochabamba, Bolivia, to characterize the mechanism involved in the resistance to clinically relevant antibiotics. Extended Spectrum β-lactamase encoding genes and plasmid-mediated quinolone resistance (PMQR) markers were investigated in a total of 101 oxyimino-cephalosporin-resistant enterobacteria recovered from different health centers during four months (2012?2013). CTX-M enzymes were detected in all isolates, being the CTX-M-1 group the most prevalent (88.1%). The presence of blaOXA-1 was detected in 76.4% of these isolates. A high quinolone resistance rate was observed among the included isolates. The aac(6′)-Ib-cr gene was the most frequent PMQR identified (83.0%). Furthermore, 6 isolates harbored the qnrB gene. Interestingly, qepA1 (6) and oqxAB (1), were detected in 7 Escherichia coli, being the latter the first to be reported in Bolivia. This study constitutes the first molecular survey on resistance markers in clinical enterobacterial isolates in Cochabamba, Bolivia, contributing to the regional knowledge of the epidemiological situation. The molecular epidemiology observed herein resembles the scene reported in South America.Fil: Saba Villarroel, Paola M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gutkind, Gabriel Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Di Conza, José Alejandro. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Radice, Marcela Alejandra. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Fractional multiphase hereditary materials: Mellin Transforms and Multi-Scale Fractances

The rheological features of several complex organic natural tissues such as bones, muscles as well as of complex artificial polymers are well described by power-laws. Indeed, it is well-established that the time-dependence of the stress and the strain in relaxation/creep test may be well captured by power-laws with exponent β ∈ [0, 1]. In this context a generalization of linear springs and linear dashpots has been introduced in scientific literature in terms of a mechanical device dubbed spring-pot. Recently the authors introduced a mechanical analogue to spring-pot built upon a proper arrangements of springs and dashpots that results in Elasto-Viscous (EV) materials, as β ∈ [0, 1/2] and Visco-Elastic ones, as β ∈ [1/2, 1]. In this paper the authors will discuss the rheological description of the presence of multiple material phases that is highlighted by a linear combination of power-laws in the relaxation function G(t) with different exponents. Such rehological model is represented by a linear combination of fractional derivatives with different order and the inverse relations have been formulated in terms of the complex method Mellin transform. Additionally an alternative representation of direct and inverse relations of multi-phase fractional hereditary materials based on the exact mechanical description of spring-pot element will be discussed in the course of the paper

Fractional differential equations of multiphase hereditary materials and exact mechanical models

Creep and relaxation tests, performed on various materials like polymers, rubbers and so on are well-tted by power-laws with exponent 2 [0; 1] (Nutting (1921), Di Paola et al. (2011)). The consequence of this observation is that the stress-strain relation of hereditary materials is ruled by fractional operators (Scott Blair (1947), Slonimsky (1961)). A large amount of researches have been performed in the second part of the last century with the aim to connect constitutive fractional relations with some mechanical models by means of fractance trees and ladders (see Podlubny (1999)). Recently, Di Paola and Zingales (2012) proposed a mechanical model that corresponds to fractional stress-strain relation with any real exponent and they have proposed a description of above model (Di Paola et al. (2012)). In this study the authors aim to extend the study to cases with more fractional phases and to fractional Kelvin-Voigt model of hereditariness

Interpreting the simultaneous variability of near-IR continuum and line emission in young stellar objects

We present new near-infrared (IR) spectra (0.80-1.35um) of the pre-Main Sequence source PV Cep taken during a monitoring program of eruptive variables we are conducting since some years. Simultaneous photometric and spectroscopic observations are systematically carried out during outburst and quiescence periods. By correlating extinction-free parameters, such as HI recombination lines and underlying continuum, it is possible to infer on the mechanism(s) responsible for their origin. Accretion and mass loss processes have a dominant role in determining the PV Cep irregular variability of both continuum and line emission. The potentialities of the observational modality are also discussed.Comment: accepted by Astrophysics and Space Scenc

Nod2 Deficiency in mice is Associated with Microbiota Variation Favouring the Expansion of mucosal CD4+ LAP+ Regulatory Cells

Nucleotide-binding Oligomerization Domain-2 (NOD2) mutations are associated with an increased risk to develop Crohn's Disease. In previous studies, we have shown that Nod2-/- mice manifest increased proportion of Lamina Propria (LP) CD4+ LAP+ Foxp3- regulatory cells, when compared with Nod2+/+ mice, while CD4+ Foxp3 + regulatory cells were not affected. Here, we investigated the Nod2 gut microbiota, by 16S rRNA pyrosequencing, at steady state and after TNBS-colitis induction in mice reared separately or in cohousing, correlating the microbial profiles with LP regulatory T cells proportion and tissue cytokines content. We found that enrichment of Rikenella and Alistipes (Rikenellaceae) in Nod2-/- mice at 8 weeks of age reared separately was associated with increased proportion of CD4+ LAP+ Foxp3- cells and less severe TNBS-colitis. In co-housed mice the acquisition of Rickenellaceae by Nod2+/+ mice was associated with increased CD4+ LAP+ Foxp3- proportion and less severe colitis. Severe colitis was associated with enrichment of gram-negative pathobionts (Escherichia and Enterococcus), while less severe colitis with protective bacteria (Barnesiella, Odoribacter and Clostridium IV). Environmental factors acting on genetic background with different outcomes according to their impact on microbiota, predispose in different ways to inflammation. These results open a new scenario for therapeutic attempt to re-establish eubiosis in Inflammatory Bowel Disease patients with NOD2 polymorphisms

GALNT2 as a novel modulator of adipogenesis and adipocyte insulin signaling

Background/objectives: A better understanding of adipose tissue biology is crucial to tackle insulin resistance and eventually coronary heart disease and diabetes, leading causes of morbidity and mortality worldwide. GALNT2, a GalNAc-transferase, positively modulates insulin signaling in human liver cells by down-regulating ENPP1, an insulin signaling inhibitor. GALNT2 expression is increased in adipose tissue of obese as compared to that of non-obese individuals. Whether this association is secondary to a GALNT2-insulin sensitizing effect exerted also in adipocytes is unknown. We then investigated in mouse 3T3-L1 adipocytes the GALNT2 effect on adipogenesis, insulin signaling and expression levels of both Enpp1 and 72 adipogenesis-related genes. Methods: Stable over-expressing GALNT2 and GFP preadipocytes (T 0 ) were generated. Adipogenesis was induced with (R+) or without (R−) rosiglitazone and investigated after 15 days (T 15 ). Lipid accumulation (by Oil Red-O staining) and intracellular triglycerides (by fluorimetric assay) were measured. Lipid droplets (LD) measures were analyzed at confocal microscope. Gene expression was assessed by RT-PCR and insulin-induced insulin receptor (IR), IRS1, JNK and AKT phosphorylation by Western blot. Results: Lipid accumulation, triglycerides and LD measures progressively increased from T 0 to T 15 R- and furthermore to T 15 R+. Such increases were significantly higher in GALNT2 than in GFP cells so that, as compared to T 15 R+GFP, T 15 R- GALNT2 cells showed similar (intracellular lipid and triglycerides accumulation) or even higher (LD measures, p < 0.01) values. In GALNT2 preadipocytes, insulin-induced IR, IRS1 and AKT activation was higher than that in GFP cells. GALNT2 effect was totally abolished during adipocyte maturation and completely reversed at late stage maturation. Such GALNT2 effect trajectory was paralleled by coordinated changes in the expression of Enpp1 and adipocyte-maturation key genes. Conclusions: GALNT2 is a novel modulator of adipogenesis and related cellular phenotypes, thus becoming a potential target for tackling the obesity epidemics and its devastating sequelae

An explicit mechanical interpretation of Eringen non-local elasticity by means of fractional calculus

If the attenuation function of strain is expressed as a power law, the formalism of fractional calculus may be used to handle Eringen non-local elastic model. Aim of the present paper is to provide a mechanical interpretation to this non-local fractional elastic model by showing that it is equivalent to a discrete, point-spring model. A one-dimensional geometry is considered; static, kinematic and constitutive equations as well as the proper boundary conditions are derived and discussed