Evaluation of growth and yield attributing characteristics of indigenous Boro rice varieties

A field experiment was conducted to evaluate the growth, yield and yield attributing characteristics of 12 indigenous Boro rice varieties collected from South-Western regions of Bangladesh namely, Nayon moni, Tere bale, Bere ratna, Ashan boro, Kajol lata, Koijore, Kali boro, Bapoy, Latai balam, Choite boro, GS one and Sylhety boro. The experiment was laid out in a Randomized Complete Block Design (RCBD) with three replicates. Parameters on, growth parameter viz. plant height and number of tillers hill-1 (at different days after transplanting); yield contributing characters such as effective tillers hill⁻¹, panicle length, number of grains panicle⁻¹, filled grains panicle1, thousand grain weight, grain yield, straw yield, biological yield and harvest index were recorded. The plant height and number of tillers hill⁻¹ at different days after transplanting varied significantly among the varieties up to harvest. At harvest, the tallest plant (123.80 cm) was recorded in Bapoy and the shortest (81.13 cm) was found in GS one. The maximum number of tillers hill⁻¹ (46.00) was observed in Sylhety boro and the minimum (19.80) in Bere ratna. All of the parameters of yield and yield contributing characters differed significantly at 1% level except grain yield, biological yield and harvest index. The maximum number of effective tillers hill⁻¹ (43.87) was recorded in the variety Sylhety boro while Bere ratna produced the lowest effective tillers hill⁻¹ (17.73). The highest (110.57) and the lowest (42.13) number of filled grains panicle⁻¹ was observed in the variety Koijore and Sylhety boro, respectively. Thousand grain weight was the highest (26.35g) in Kali boro and the lowest (17.83g) in GS one. Grain did not differ significantly among the varieties but numerically the highest grain yield (5.01 t ha⁻¹) was found in the variety Koijore and the lowest in GS one (3.17 t ha⁻¹)

Proses Pembuatan Kerupuk opak Singkong yang Renyah dan Gurih untuk Bisnis UMKM

Opak singkong adalah olahan krupuk yang berbahan dasar singkong. Pada proses pengolahan singkong menjadi opak masih menerapkan bentuk kegiatan produksi yang sederhana dan belum melakukan inovasi dalam produksi. Pengabdian masyarakat ini bertujuan untuk meningkatkan keterampilan masyarakat dalam mengolah singkong, walaupun kegiatan telah usai peserta dapat melakukan sendiri dan memiliki inisiatif untuk pengembangan produk. Kegiatan ini dilaksanakan pada tanggal 7 Februari 2025 di Desa Senuro Timur, Kecamatan Tanjung Batu, Kabupaten Ogan Ilir. Pendekatan yang digunakan dalam pengabdian kepada masyarakat ini adalah metode partisipatif, yang menekankan keterlibatan aktif pelaku UMKM dalam perencanaan, pelaksanaan, hingga evaluasi dalam pembuatan kerupuk opak singkong. Mahasiswa KKN melakukan diskusi kelompok membahas pelaksanaan secara langsung pembuatan keripuk opak singkong. Hasil dari pelaksanaan kegiatan pengabdian ini yaitu dapat meningkatkan pengetahuan dan kemampuan masyarakat mengenai pengembangan produk pangan serta dapat melanjutkan dan mengembangkan secara mandiri usaha ini karena memiliki peluang pasar dengan nilai jual yang tinggi

Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species

Many herbicide-resistant weed species are polyploids, but far too little about the evolution of resistance mutations in polyploids is understood. Hexaploid wild oat (Avena fatua) is a global crop weed and many populations have evolved herbicide resistance. We studied plastidic acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide resistance in hexaploid wild oat and revealed that resistant individuals can express one, two or three different plastidic ACCase gene resistance mutations (Ile-1781-Leu, Asp-2078-Gly and Cys-2088-Arg). Using ACCase resistance mutations as molecular markers, combined with genetic, molecular and biochemical approaches, we found in individual resistant wild-oat plants that (1) up to three unlinked ACCase gene loci assort independently following Mendelian laws for disomic inheritance, (2) all three of these homoeologous ACCase genes were transcribed, with each able to carry its own mutation and (3) in a hexaploid background, each individual ACCase resistance mutation confers relatively low-level herbicide resistance, in contrast to high-level resistance conferred by the same mutations in unrelated diploid weed species of the Poaceae (grass) family. Low resistance conferred by individual ACCase resistance mutations is likely due to a dilution effect by susceptible ACCase expressed by homoeologs in hexaploid wild oat and/or differential expression of homoeologous ACCase gene copies. Thus, polyploidy in hexaploid wild oat may slow resistance evolution. Evidence of coexisting non-target-site resistance mechanisms among wild-oat populations was also revealed. In all, these results demonstrate that herbicide resistance and its evolution can be more complex in hexaploid wild oat than in unrelated diploid grass weeds. Our data provide a starting point for the daunting task of understanding resistance evolution in polyploids

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase 1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age  6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score  652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc = 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N = 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in Asia and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701

Ser-653-Asn substitution in the acetohydroxyacid synthase gene confers resistance in weedy rice to imidazolinone herbicides imazapic and imazapyr in Malaysia

AbstractThe IMI-herbicides rice package has been recognized by all means among the most efficient chemical approaches for weedy rice control nowadays. Inevitably, the continuous and sole dependence, as well as ignorance on the appropriate use of imidazolinone herbicides in the IMI-herbicides rice package by rice growers has caused the development of herbicide resistance in weedy rice populations across many IMI-herbicides rice package adopted countries, inclusive of Malaysia. Hence, a comprehensive study was conducted to elucidate the occurrence, level, and mechanisms endowing resistance to IMI-herbicides on field-reported resistant (R) weedy rice populations collected from IMI-rice fields in Kampung Simpang Sanglang, Perlis (A), Kampung Behor Mentalon, Perlis (B), and Kampung Sungai Kering, Kedah (C). The collected weedy rice populations were compared with a susceptible weedy rice population (S), an imidazolinone-resistant rice cultivar (IMI-rice), and a susceptible local rice cultivar (MR219). Dose-response experiments were carried out using commercial IMI-herbicides (premix of imazapic and imazapyr) available in the IMI-herbicides rice package, in the seed bioassay and whole-plant dose-response. Based on the Resistance Index (RI) quantification in both experiments, the cross-resistance pattern of weedy rice populations and rice varieties to imazapic and imazapyr was determined. Molecular investigation was carried out by comparing acetohydroxyacid synthase (AHAS) gene sequences between resistant (R) weedy rice populations (A, B, and C), S population, IMI-rice, and MR219. Evidently, the AHAS gene sequences of R weedy rice were identical to the IMI-rice, revealing that amino acid substitution of Ser-653-Asn occurs in both R populations and IMI-rice, but neither in MR219 nor S plants. In vitro assays were conducted using analytical grade imidazolinone herbicides of imazapic (99.3%) and imazapyr (99.6%) with seven concentrations. The results demonstrated that the AHAS enzyme extracted from R populations and IMI-rice were less sensitive to IMI-herbicides in comparison to S and MR219, further supporting the IMI-herbicides resistance was conferred by target site mutation. In conclusion, the basis of imidazolinone resistance in selected populations of Malaysia weedy rice was due to a Ser-653-Asn mutation that reduced sensitivity of the target site to IMI-herbicides. The current study presents the first report of resistance mechanism in weedy rice in Malaysian rice fields.</jats:p

ACCase-Inhibiting Herbicide-Resistant<i>Avena</i>spp. Populations from the Western Australian Grain Belt

Avenaspp. are world weeds with many cases of evolved herbicide resistance. In Australia,Avenaspp. (wild oat and sterile oat) are a major problem, especially in grain crops. Acetyl-CoA carboxylase (ACCase)–inhibiting herbicides have been used extensively since the late 1970s forAvenaspp. control. However, continued reliance on these herbicides has resulted in the evolution of resistantAvenaspp. populations. Resistance across many ACCase-inhibiting herbicides was characterized in fourAvenaspp. populations from the Western Australian grain belt. Dose–response experiments were conducted to determine the level of resistance to the aryloxyphenoxypropionates and cyclohexanediones and to the phenylpyrazoline herbicide pinoxaden. On the basis of resistance index values, all four resistant populations exhibited high-level diclofop resistance but varied in the level of resistance to other ACCase-inhibiting herbicides tested. It is evident thatAvenaspp. populations from the Western Australian grain belt have evolved resistance to a number of ACCase-inhibiting herbicides.</jats:p

Data from: Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): Insights into resistance evolution in a hexaploid species

Many herbicide-resistant weed species are polyploids, but far too little about the evolution of resistance mutations in polyploids is understood. Hexaploid wild oat (Avena fatua) is a global crop weed and many populations have evolved herbicide resistance. We studied plastidic acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicide resistance in hexaploid wild oat and revealed that resistant individuals can express one, two or three different plastidic ACCase gene resistance mutations (Ile-1781-Leu, Asp-2078-Gly and Cys-2088-Arg). Using ACCase resistance mutations as molecular markers, combined with genetic, molecular and biochemical approaches, we found in individual resistant wild-oat plants that (1) up to three unlinked ACCase gene loci assort independently following Mendelian laws for disomic inheritance, (2) all three of these homoeologous ACCase genes were transcribed, with each able to carry its own mutation and (3) in a hexaploid background, each individual ACCase resistance mutation confers relatively low-level herbicide resistance, in contrast to high-level resistance conferred by the same mutations in unrelated diploid weed species of the Poaceae (grass) family. Low resistance conferred by individual ACCase resistance mutations is likely due to a dilution effect by susceptible ACCase expressed by homoeologs in hexaploid wild oat and/or differential expression of homoeologous ACCase gene copies. Thus, polyploidy in hexaploid wild oat may slow resistance evolution. Evidence of coexisting non-target-site resistance mechanisms among wild-oat populations was also revealed. In all, these results demonstrate that herbicide resistance and its evolution can be more complex in hexaploid wild oat than in unrelated diploid grass weeds. Our data provide a starting point for the daunting task of understanding resistance evolution in polyploids

Fig. 5. Plastidic ACCase gene partial sequence alignment

Partial sequence alignment (from cloned fragments) showing the three haplotype sequences of the 2088, 1781 and 2078 mutations identified in the present study, corresponding respectively to the ACCase gene copies Acc1;1, Acc1;2, and Acc1;3 in wild oat (Christoffers et al., 2002). Note that the sequence region containing the 1781, 2078 or 2088 codon was not included due to infrequent informative SNPs. Boxed regions indicate SNPs discriminating the three haplotypes (GenBank accession AF231335 for Acc1;1-1, AF231334 for Acc1;1-2, AF231336 for Acc1;2 and AF464875 for Acc1;3)

Fig. S4( d)CAPS analysis of clones

Fig. S4 (d)CAPS marker analysis of ACCase mutations at (a) 1781, (b) 2078 or (c) 2088 in representative E. coli clones containing the 1122 kb ACCase cDNA insert. Each lane represents a single clone analysed by each of the three markers. Note that none of these clones contain more than one mutation (R)