INVESTIGATION OF GENETIC DIVERSITY, PHYLOGENY AND SUB-SPECIES STRUCTURING IN THE ENTOMOPATHOGENIC NEMATODE HETERORHABDITIS

We used Random amplified polymorphic DNA (RAPDs), partial ribosomal DNA (rDNA), the mitochondrial gene cytochrome oxidase subunit I (cox1) and major sperm protein (msp) sequence analysis to investigate genetic diversity and population structure in Heterorhabditis bacteriophora, H. indica, H. marelata, H. megidis, H. downesi and H. zealandica comprising 18 isolates collected from different parts of the world. Blastn similarity search performed for the partial rDNA sequences confirmed the identity of Heterorhabditis species and suggested that all the unknown isolates belonged to H. bacteriophora. Blastn e-values for the species and isolates ranged between 0 to 9e-145. Genetic diversity and phylogenetic analysis produced dendrograms that showed high degrees of genetic variations among Heterorhabditis species with the overall average pairwise distance values of 0.3217, 0.6391, 0.7963 and 0.0572 for RAPD, partial rDNA, cox1 and msp, respectively. Although we expected low genetic diversity among H. bacteriophora isolates due to alternate automictic and amphimictic lifecycle and lack of long distance movement capability restricting gene flow, our results demonstrate highly structured genetic variation among the isolates. Phylogenetic analysis showed that H. bacteriophora isolates is a species complex that contain at least two two new species KMD10 and GPS5. Further H. bacteriophora sensu Pionar populations can be divided into two major groups: “HP88” and “Oswego”. We conclude that strictly relying on ITS sequences based blastn for Heterorhabditis species identification is misleading.OARD

Thyroid exposure in brain CT-scan and skull X-ray, using different levels of mA and Kvp with and without thyroid shield.

زمینه و هدف: امروزه در تصویر برداری های رادیولوژیکی از جمجمه، پرتو گیری تیروئید، به عنوان یک عضو بحرانی، اهمیت فراوانی دارد. این مطالعه با هدف بررسی دز دریافتی تیروئید در دو روش سی تی اسکن مغز و رادیوگرافی معمول جمجمه، با اعمال مقادیر مختلف شدت جریان و اختلاف پتانسیل با و بدون استفاده از شیلد تیروئید انجام شد. روش بررسی: در این مطالعه توصیفی- تحلیلی 350 بیمارسرپایی انتخاب و قبل از پرتودهی 2 عدد قرص TLD (Thermo luminescence Dosimetr) بر روی تیروئید هر بیمار نصب شد. برای انجام سی تی اسکن مغز سه سطح مختلف شدت جریان (در 210 بیمار) و برای رادیوگرافی معمول جمجمه دو سطح مختلف اختلاف پتانسیـــل (در 140 بیمار) استفاده گردید. پرتودهی ها با و بدون استفاده از شیلد تیروئید انجام شد. سپس TLD ها قرائت و داده به کمک آزمون آماری t دانشجویی تجزیه و تحلیل شدند. یافته ها: در آزمون های سی تی اسکن (با Kvp ثابت)، با کاهش شدت جریان از 150 به 125 میلی آمپر میزان پرتوگیری تیروئید از 38/9±101 به 04/8±2/82 میلی رم رسید (01/0

Dose assessment and radioactivity of the mineral water resources of Dimeh springs in the Chaharmahal and Bakhtiari Province, Iran

One of the main sources of public exposure from natural radioactivity is radium and radon and its short lived decay products. The aim of this study is focused on determining the concentration of mentioned radionuclides in the mineral water resources in one of the highest altitude regions (Zagros Mountains), the Chaharmahal and Bakhtiari province, in South-West Iran, namely the Dimeh springs. Eighty drinking spring water samples were taken from the Dimeh springs (ten samples for each spring) to determine radium-226 ( 226Ra) and radon-222 (222Rn) concentrations using the emanation method and a liquid scintillation counting method, respectively. The results of this study showed that the effective dose from the consumption of drinking water of Dimeh springs (6.4 μSv/y) is not comparable to the other annual effective dose such as inhalation of radon and its products in cosmic and terrestrial rays published by one of the authors previously. Also, the annual effective dose measured here is much lower than the worldwide mean value 0.45 mSv reported by USCEAR, 2000 and is also low enough and below the proposed limits in other countries and this is the reason why this sources of water may be considered as safe drinking water in the region

Problems Experienced by Adolescents with Autism Spectrum Disorder: Exploring Social, Educational and Economic Variables

The study investigates the problems of adolescents with Autism Spectrum Disorder (ASD) from the perspective of ASD specialists and adolescent’s families. It comprises of 228 ASD specialists and 294 families of ASD adolescents. The sample represents all areas of the Kingdom of Saudi Arabia (henceforth – KSA): north, south, east, west and centre. Variables of the study are adolescents’ gender, age, residential area, as well as the qualification of ASD specialists and family members. To accomplish the study, the researchers designed a questionnaire that includes variables regarding six significant problems: Educational, Recreational, Economic, Health, Psychological, and Social. The results reveal that families rate the economic problems higher than average, while the recreational ones – lower; specialists rate economic problems higher than average and educational ones – the lowest. As for gender variable, specialists and families note the differences in favour of females; for age variable, specialists and families note that 15-18 years old ASD adolescents have more problems. The results show that qualification of specialists as well as family members is of significant relevance in relation to ASD adolescents. Both specialists and family members are mostly from the central regions

Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids

Treatment of common bile duct disorders such as biliary atresia or ischaemic strictures is limited to liver transplantation or hepatojejunostomy due to the lack of suitable tissue for surgical reconstruction. Here, we report a novel method for the isolation and propagation of human cholangiocytes from the extrahepatic biliary tree and we explore the potential of bioengineered biliary tissue consisting of these extrahepatic cholangiocyte organoids (ECOs) and biodegradable scaffolds for transplantation and biliary reconstruction in vivo. ECOs closely correlate with primary cholangiocytes in terms of transcriptomic profile and functional properties (ALP, GGT). Following transplantation in immunocompromised mice ECOs self-organize into tubular structures expressing biliary markers (CK7). When seeded on biodegradable scaffolds, ECOs form tissue-like structures retaining biliary marker expression (CK7) and function (ALP, GGT). This bioengineered tissue can reconstruct the wall of the biliary tree (gallbladder) and rescue and extrahepatic biliary injury mouse model following transplantation. Furthermore, it can be fashioned into bioengineered ducts and replace the native common bile duct of immunocompromised mice, with no evidence of cholestasis or lumen occlusion up to one month after reconstruction. In conclusion, ECOs can successfully reconstruct the biliary tree following transplantation, providing proof-of-principle for organ regeneration using human primary cells expanded in vitro

Folliculogenesis and follicular fluid adiponectin in cows: Its alterations and relationships with ovarian function [Folikulogeneza i adiponektin u folikularnoj tekućini krava: Promjene i odnos s funkcijom jajnika]

The objectives of the present study were to study the dynamic changes in adiponectin concentration in the growing luteal as well as the preovulatory follicles in dairy cows. In the first study, the ovaries and blood of 15 Holstein dairy cows in the luteal phase were collected from a slaughterhouse. Clear antral follicles were divided into three diameter groups (small, 3-5 mm; medium, 6-9 mm and large, ≥10 mm) and their fluid was aspirated. In the second study, the coccygeal blood and fluid of the preovulatory follicles of eight live Holstein dairy cows were aspirated transrectally, using a transrectal-guided fine-needle. Concentrations of adiponectin in the serum, and follicular fluid and progesterone in the serum were measured. Serum adiponectin concentrations in both luteal and follicular phases were higher than the follicular fluid adiponectin concentrations in all types of follicles (P0.05), and the reduction was seen in preovulatory follicles in comparison with small follicles (P = 0.001). In the luteal phase, a significant positive correlation was observed between the adiponectin concentrations in different sized follicles, and also in the serum progesterone and follicular fluid adiponectin of follicles (P<0.05). In conclusion, lower adiponectin concentrations in blood serum and preovulatory follicles in comparison to luteal growing follicles reflect the effect of ovarian stage on adiponectin alterations

Analysis of advanced supercritical carbon dioxide power cycles with a bottoming cycle for concentrating solar power applications

A number of studies have been performed to assess the potential of using supercritical carbon dioxide (S-CO 2 ) in closed-loop Brayton cycles for power generation. Different configurations have been examined among which recompression and partial cooling configurations have been found very promising, especially for concentrating solar power (CSP) applications. It has been demonstrated that the S-CO 2 Brayton cycle using these configurations is capable of achieving more than 50% efficiency at operating conditions that could be achieved in central receiver tower type CSP systems. Although this efficiency is high, it might be further improved by considering an appropriate bottoming cycle utilizing waste heat from the top S-CO 2 Brayton cycle. The organic Rankine cycle (ORC) is one alternative proposed for this purpose; however, its performance is substantially affected by the selection of the working fluid. In this paper, a simple S-CO 2 Brayton cycle, a recompression S-CO 2 Brayton cycle, and a partial cooling S-CO 2 Brayton cycle are first simulated and compared with the available data in the literature. Then, an ORC is added to each configuration for utilizing the waste heat. Different working fluids are examined for the bottoming cycles and the operating conditions are optimized. The combined cycle efficiencies and turbine expansion ratios are compared to find the appropriate working fluids for each configuration. It is also shown that combined recompression-ORC cycle achieves higher efficiency compared with other configurations

A Unifying Mechanism for Mitochondrial Superoxide Production during Ischemia-Reperfusion Injury.

Ischemia-reperfusion (IR) injury occurs when blood supply to an organ is disrupted--ischemia--and then restored--reperfusion--leading to a burst of reactive oxygen species (ROS) from mitochondria. It has been tacitly assumed that ROS production during IR is a non-specific consequence of oxygen interacting with dysfunctional mitochondria upon reperfusion. Recently, this view has changed, suggesting that ROS production during IR occurs by a defined mechanism. Here we survey the metabolic factors underlying IR injury and propose a unifying mechanism for its causes that makes sense of the huge amount of disparate data in this area and provides testable hypotheses and new directions for therapies.Work in our laboratories is supported by the Medical Research Council (UK) and the British Heart Foundation. E.T.C. is supported by a Human Frontiers Science Program fellowship.This is the author accepted manuscript. The final version is available from Cell Press via http://dx.doi.org/10.1016/j.cmet.2015.12.00

A secure key distribution protocol based on hash functions and a quantum-authenticated channel using 6DP (KDP-6DP)

Security is the most tedious problem in highly sensitive communications. Quantum security is the key issue in solving the problem. A key distribution protocol based on the use of hash functions is proposed. The essential part of the protocol depends on sending a string of random characters from sender to receiver. Then, a selected hash or a cascade of two hash functions and a long-term shared secret are used to construct the key. Consequently, the session key is generated on-site by independently applying a hash function on the random string at the sender and receiver sides. This protocol requires a reliable method of authentication. Therefore, it is further proposed to use an out-of-band authentication methodology based on the deterministic six-state quantum authentication protocol that is referred to as 6DP