Formulation and standardization of homoeopathic mother tincture of Cinnamomum zeylanicum

Alcoholic preparation of drugs in Homoeopathy, are called as tincture. The mother tincture of Cinnamomum zeylanicum was prepared by 100 g of coarse powder (40 mess) of Cinnamomum zeylanicum was macerated with 95 v/v % of alcohol to make 1000 ml of mother tincture for eight days (drug strength 1/10). It was shaken occasionally, and filtered. The mother tincture was standardized by using various physical, chemical and chromatographic parameters.Keywords: Cinnamomum zeylanicum, mother tinctur

Periodontitis as a causative agent for systemic disease of pancreas

In recent years it has been studied that periodontal diseases have a strong correlation with systemic health. The purpose of the present study is to correlate the periodontitis with the pancreatic health. Two hundred sixty subjects were selected for this study and divided in control and chronic periodontitis. Amylase, lipase, glucose, cholesterol, sodium, potassium and calcium level were measured in serum of all subjects. Significant changes were observed in amylase, glucose, cholesterol and calcium levels in periodontitis subjects

Formulation and standardization of homoeopathic mother tincture of Cinnamomum zeylanicum

Alcoholic preparation of drugs in Homoeopathy, are called as tincture. The mother tincture of Cinnamomum zeylanicum was prepared by 100 g of coarse powder (40 mess) of Cinnamomum zeylanicum was macerated with 95 v/v % of alcohol to make 1000 ml of mother tincture for eight days (drug strength 1/10). It was shaken occasionally, and filtered. The mother tincture was standardized by using various physical, chemical and chromatographic parameters

Comparison between caudal epidural block and popliteal nerve block for postoperative analgesia in children undergoing foot surgery: a randomized controlled trial

Background: Pain following surgery in children cause discomfort, restlessness and agitation in the postoperative period which may result in an increased incidence of nausea, vomiting and maladaptive behavioural changes. Regional anaesthesia is commonly used as an adjunct to general anaesthesia for perioperative analgesia in children as part of a multimodal approach of pain relief. This study is to compare between caudal epidural block and popliteal nerve block for postoperative analgesia in children undergoing foot surgery.Methods: A prospective randomized single blind study was carried out on 30 children aged 1-12 years of either sex undergoing foot surgery. Patients were randomly assigned into caudal epidural block group and Popliteal nerve block group, 15 children each. Both groups receive 1 ml/kg of 0.25% bupivacaine. Foot surgery was carried out under general anesthesia along with regional block for all children. After completion of surgery, children were shifted to PACU and HR, BP, SPO2 were monitored. Patient was discharged from PACU after CHEOPS (1-5 years) or VAS (6-12 years) <4. Parental satisfaction, sedation score, PONV, and any other side effects were recorded.Results: Demographic data and baseline vital signs were comparable between two groups. Statistically significant difference (p=0.025) in number of attempts in giving block in group A (1.20±0.41) than group B (1.80±0.86). The mean postoperative pain scores, CHEOPS and VAS were comparable in both groups.Conclusions: Both caudal epidural block and popliteal nerve block provides comparable and adequate analgesia in children undergoing elective foot surgery

Therapeutic implications of current Janus kinase inhibitors as anti-COVID agents: A review

Severe cases of COVID-19 are characterized by hyperinflammation induced by cytokine storm, ARDS leading to multiorgan failure and death. JAK-STAT signaling has been implicated in immunopathogenesis of COVID-19 infection under different stages such as viral entry, escaping innate immunity, replication, and subsequent inflammatory processes. Prompted by this fact and prior utilization as an immunomodulatory agent for several autoimmune, allergic, and inflammatory conditions, Jakinibs have been recognized as validated small molecules targeting the rapid release of proinflammatory cytokines, primarily IL-6, and GM-CSF. Various clinical trials are under investigation to evaluate Jakinibs as potential candidates for treating COVID-19. Till date, there is only one small molecule Jakinib known as baricitinib has received FDA-approval as a standalone immunomodulatory agent in treating critical COVID-19 patients. Though various meta-analyses have confirmed and validated the safety and efficacy of Jakinibs, further studies are required to understand the elaborated pathogenesis of COVID-19, duration of Jakinib treatment, and assess the combination therapeutic strategies. In this review, we highlighted JAK-STAT signalling in the pathogenesis of COVID-19 and clinically approved Jakinibs. Moreover, this review described substantially the promising use of Jakinibs and discussed their limitations in the context of COVID-19 therapy. Hence, this review article provides a concise, yet significant insight into the therapeutic implications of Jakinibs as potential anti-COVID agents which opens up a new horizon in the treatment of COVID-19, effectively

Integrating network pharmacology with molecular docking to rationalize the ethnomedicinal use of Alchornea laxiflora (Benth.) Pax & K. Hoffm. for efficient treatment of depression

Background: Alchornea laxiflora (Benth.) Pax &amp; K. Hoffm. (A. laxiflora) has been indicated in traditional medicine to treat depression. However, scientific rationalization is still lacking. Hence, this study aimed to investigate the antidepressant potential of A. laxiflora using network pharmacology and molecular docking analysis.Materials and methods: The active compounds and potential targets of A. laxiflora and depression-related targets were retrieved from public databases, such as PubMed, PubChem, DisGeNET, GeneCards, OMIM, SwissTargetprediction, BindingDB, STRING, and DAVID. Essential bioactive compounds, potential targets, and signaling pathways were predicted using in silico analysis, including BA-TAR, PPI, BA-TAR-PATH network construction, and GO and KEGG pathway enrichment analysis. Later on, with molecular docking analysis, the interaction of essential bioactive compounds of A. laxiflora and predicted core targets of depression were verified.Results: The network pharmacology approach identified 15 active compounds, a total of 219 compound-related targets, and 14,574 depression-related targets with 200 intersecting targets between them. SRC, EGFR, PIK3R1, AKT1, and MAPK1 were the core targets, whereas 3-acetyloleanolic acid and 3-acetylursolic acid were the most active compounds of A. laxiflora with anti-depressant potential. GO functional enrichment analysis revealed 129 GO terms, including 82 biological processes, 14 cellular components, and 34 molecular function terms. KEGG pathway enrichment analysis yielded significantly enriched 108 signaling pathways. Out of them, PI3K-Akt and MAPK signaling pathways might have a key role in treating depression. Molecular docking analysis results exhibited that core targets of depression, such as SRC, EGFR, PIK3R1, AKT1, and MAPK1, bind stably with the analyzed bioactive compounds of A. laxiflora.Conclusion: The present study elucidates the bioactive compounds, potential targets, and pertinent mechanism of action of A. laxiflora in treating depression. A. laxiflora might exert an antidepressant effect by regulating PI3K-Akt and MAPK signaling pathways. However, further investigations are required to validate

Patterns of Use of Oral Rehydration Therapy in Srinagar (Garhwal), Uttaranchal, India

A study was conducted to assess the knowledge of preparing packet oral rehydration solution (ORS) and home-made salt-sugar solution (SSS) among mothers in Srinagar (Garhwal), Uttaranchal. Two hundred and twenty-five mothers were interviewed. Only a small proportion recognized the ORS packets (18.66%) and only 17.77% mentioned the correct method of preparing a solution from a packet (even after reading the instructions on the packet). Home-made SSS was adequately discussed by only 6.22% mothers and they were taught to correctly prepare and administer ORS and home-made SSS. After the educational programme, significant ( P&lt;0.001) improvement in their knowledge was found. Eighty-six per cent knew the correct method of preparing packet ORS and 80.88% the correct method of preparing home-made SSS ( P&lt;0.001). Interventions of this kind should be carried out to improve the knowledge and skills of mothers in treating childhood diarrhoea. </jats:p

Prescription Audit: Experience in Garhwal (Uttaranchal), India

A total of 400 prescriptions were randomly audited from Govern Combined Hospital, Srinagar (Garhwal). Various prescribing specific indicators (recommended by World Health Organization and others) were studied and patient compliance was measured. All of the prescriptions were written for outpatients (males 59.25%; females 40.75%). The average number of drugs prescribed was 3.65 and about 51% of the drugs were prescribed by generic names. NSAIDs were the most widely prescribed (89.75%), antibiotics (77.25%), and vitamins (59.74%) in various clinical conditions. The diagnosis was mentioned only in 22.25% of the prescriptions. A total of 59% fixed dose combinations (FDCs) were prescribed. The patient compliance was 71.5%. The injection use was found to be 7% only. Our survey reveals increased drug exposure to the patients, indiscriminate use of NSAIDs, antibiotics and vitamins. Further studies in this area using a larger sample size should be carried out, and a well designed training programme should be conducted on rational drug use. </jats:p

In Vitro Cytotoxicity of Nanoparticles: A Comparison between Particle Size and Cell Type

The reduction in size of Zinc oxide (ZnO) and Silicon dioxide (SiO2) particles from micron to nano scale offers unique physical characteristics on one hand while making them cytotoxic on other hand. The present study was aimed at comparing cytotoxic effects of ZnO and SiO2 nanoparticles with their micron size and secondary aim was to compare responses of these particles to two different cell types, namely, human lung epithelial cells (L-132) and human monocytes (THP-1). The L-132 and THP-1 cells were exposed to nano and micron size of ZnO and SiO2 particles with different concentrations (5–500 μg/mL) for 24 h, and cytotoxicity was analyzed by MTT assay, live-dead staining, and TC-50 was calculated. ZnO and SiO2 particles showed concentration-dependent cytotoxicity in both cell lines. In size-dependent study, ZnO particles exhibited nearly equal toxicity profile in L-132 cells while in THP-1 cells nano ZnO showed more toxicity than its micron size. The SiO2 particles showed more toxicity in their nano size than micron size in both cell lines. Human monocytes, THP-1 cells, were more sensitive towards the toxicity of both particles than human lung cells, L-132. The results highlight the difference of cytotoxicity between particle sizes and differential sensitivity of cells towards the particles of same composition. In conclusion, ZnO and SiO2 particles exhibited concentration-dependent toxicity, which was more in their nano size than micron counterpart. However, the toxic response varies depending on type of cell exposed due to differential sensitivity

Comparison between caudal epidural block and popliteal nerve block for postoperative analgesia in children undergoing foot surgery: a randomized controlled trial

Background: Pain following surgery in children cause discomfort, restlessness and agitation in the postoperative period which may result in an increased incidence of nausea, vomiting and maladaptive behavioural changes. Regional anaesthesia is commonly used as an adjunct to general anaesthesia for perioperative analgesia in children as part of a multimodal approach of pain relief. This study is to compare between caudal epidural block and popliteal nerve block for postoperative analgesia in children undergoing foot surgery.Methods: A prospective randomized single blind study was carried out on 30 children aged 1-12 years of either sex undergoing foot surgery. Patients were randomly assigned into caudal epidural block group and Popliteal nerve block group, 15 children each. Both groups receive 1 ml/kg of 0.25% bupivacaine. Foot surgery was carried out under general anesthesia along with regional block for all children. After completion of surgery, children were shifted to PACU and HR, BP, SPO2 were monitored. Patient was discharged from PACU after CHEOPS (1-5 years) or VAS (6-12 years) &lt;4. Parental satisfaction, sedation score, PONV, and any other side effects were recorded.Results: Demographic data and baseline vital signs were comparable between two groups. Statistically significant difference (p=0.025) in number of attempts in giving block in group A (1.20±0.41) than group B (1.80±0.86). The mean postoperative pain scores, CHEOPS and VAS were comparable in both groups.Conclusions: Both caudal epidural block and popliteal nerve block provides comparable and adequate analgesia in children undergoing elective foot surgery.</jats:p