Variability of Iberian upwelling implied by ERA-40 and ERA-Interim reanalyses

The Regional Ocean Modeling System ocean model is used to simulate the decadal evolution of the regional waters in offshore Iberia in response to atmospheric fields given by ECMWF ERA-40 (1961–2001) and ERA-Interim (1989–2008) reanalyses. The simulated sea surface temperature (SST) fields are verified against satellite AVHRR SST, and they are analysed to characterise the variability and trends of coastal upwelling in the region. Opposing trends in upwelling frequency are found at the northern limit, where upwelling has been decreasing in recent decades, and at its southern edge, where there is some evidence of increased upwelling. These results confirm previous observational studies and, more importantly, indicate that observed SST trends are not only due to changes in radiative or atmospheric heat fluxes alone but also due to changes in upwelling dynamics, suggesting that such a process may be relevant in climate change scenarios

Evidence for rangewide panmixia despite multiple barriers to dispersal in a marine mussel

Oceanographic features shape the distributional and genetic patterns of marine species by interrupting or promoting connections among populations. Although general patterns commonly arise, distributional ranges and genetic structure are species-specific and do not always comply with the expected trends. By applying a multimarker genetic approach combined with Lagrangian particle simulations (LPS) we tested the hypothesis that oceanographic features along northeastern Atlantic and Mediterranean shores influence dispersal potential and genetic structure of the intertidal mussel Perna perna. Additionally, by performing environmental niche modelling we assessed the potential and realized niche of P. perna along its entire native distributional range and the environmental factors that best explain its realized distribution. Perna perna showed evidence of panmixia across > 4,000 km despite several oceanographic breaking points detected by LPS. This is probably the result of a combination of life history traits, continuous habitat availability and stepping-stone dynamics. Moreover, the niche modelling framework depicted minimum sea surface temperatures (SST) as the major factor shaping P. perna distributional range limits along its native areas. Forthcoming warming SST is expected to further change these limits and allow the species to expand its range polewards though this may be accompanied by retreat from warmer areas.Fundacao para a Ciencia e Tecnologia (FCT-MEC, Portugal) [UID/Multi/04326/2013, IF/01413/2014/CP1217/CT0004]; South African Research Chairs Initiative (SARChI) of the Department of Science and Technology; National Research Foundation; South African National Research Foundation (NRF); Portuguese Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BPD/85040/2012, SFRH/BPD/111003/2015]info:eu-repo/semantics/publishedVersio

A study of Docetaxel-induced effects in MCF-7 cells by means of Raman microspectroscopy

Chemotherapies feature a low success rate of about 25%, and therefore, the choice of the most effective cytostatic drug for the individual patient and monitoring the efficiency of an ongoing chemotherapy are important steps towards personalized therapy. Thereby, an objective method able to differentiate between treated and untreated cancer cells would be essential. In this study, we provide molecular insights into Docetaxel-induced effects in MCF-7 cells, as a model system for adenocarcinoma, by means of Raman microspectroscopy combined with powerful chemometric methods. The analysis of the Raman data is divided into two steps. In the first part, the morphology of cell organelles, e.g. the cell nucleus has been visualized by analysing the Raman spectra with k-means cluster analysis and artificial neural networks and compared to the histopathologic gold standard method hematoxylin and eosin staining. This comparison showed that Raman microscopy is capable of displaying the cell morphology; however, this is in contrast to hematoxylin and eosin staining label free and can therefore be applied potentially in vivo. Because Docetaxel is a drug acting within the cell nucleus, Raman spectra originating from the cell nucleus region were further investigated in a next step. Thereby we were able to differentiate treated from untreated MCF-7 cells and to quantify the cell–drug response by utilizing linear discriminant analysis models

Bio-physical coupling around three shallow seamounts in the South Western Indian Ocean, with regional comparisons based on modelling, remote sensing and observational studies

Based on satellite and in situ data, the dynamic characteristics and vertical structure of a surface intensified mesoscale eddy dipole recently expelled from the South East Madagascar Current (SEMC) is described for the first time. The dipole was surveyed 250 nautical miles south of Madagascar between 14 and 23 November 2016, during west-east and south-north transects carried out over the northern Madagascar Ridge. The dipole consisted of two counter-rotating vortices of similar size (100 km) and intensity (0.7 f), and an intense southwestward jet (150 cm s(-1)) in the frontal region between the two eddies. The cyclonic eddy was lying on the western side of the anticyclonic eddy. With azimuthal velocities reaching 100 cm s(-1) at the surface and decreasing slowly with depth (40 cm s(-1) at -600 m), this dipole was defined as a highly non-linear (Ro similar to 0.7) isolated eddy-type structure (c(beta) similar to 11 cm s(-1) and U/c(beta) similar to 0.7) capable of trapping and advecting water masses over large distances. The enhanced concentration of chlorophyll-a found in the cyclone relative to the anticyclone could be tracked back to the spin-up phase of the two eddies and attributed to eddy-pumping. The eddy cores were located above the pycnocline (1026.4 kg m(-3)), within the upper 600 m, and consisted of varieties of Subtropical Underwater (STUW) found within the SEMC. The STUW found in the anticyclone was more saline and oxygenated than in the cyclone, highlighting mixing with the inshore shelf waters from the southeastern coastal upwelling cell off Madagascar. Observations suggest that the dipole interacted strongly with the chaotic bathymetry of the region, characterized by a group of five seamounts lying between -240 m and -1200 m. The bathymetry blocked its westward advection, trapping it in the vicinity of one shallow seamount for more than 4 weeks, so enhancing the role of the eddy-induced velocities in stirring the surrounding water masses. Squeezed between the southern Madagascan shelf and the northern flank of the anticyclone, two filament-like dynamic features with very different water-mass properties could be observed on the south-north transect: i) one filament highly concentrated in chlorophyll-a demonstrating the capacity of the eddy to export shelf water offshore; ii) intrusions of southern-type STUW generally found south of the South Indian Counter Current (SICC) recirculating on the external flanks of the anticyclone. Although the observed circulation and hydmgraphy were largely constrained by the presence of the mesoscale eddy dipole, unmistakable fine-scale dynamics were also observed in the vicinity of the MAD-Ridge seamount, superimposed onto the mesoscale eddy flow