Biosensors for Cellular Imaging on the Base of Colloidal CdMnS Nanoparticles

Semiconductor nanoparticles have rapidly found a broad range of applications as optical imaging agents. The presented paper describes the growth, analysis of optical spectra and study of cellular imaging of Mn-doped cadmium sulfide nanoparticles. Semimagnetic semiconductor nanoparticles CdMnS are characterized by high brightness, improved photostability and multicolor size- and structure-dependent light emission in the visible range of spectra. They are emerging as a new class of fluorescent reporters with properties and applications that are not available with traditional organic dyes. In order to obtain nanosensors different surfactants, growth factors and conditions such as surfactant concentrations, ionic strength, precursor concentration have been varied. Formation of nanoparticles was monitored by optical methods. The average nanoparticle size was estimated by optical spectra. For cellular research histological section of a placental tissues and liver tissues has been used. This procedure is possible only after cell fixation and permeabilization treatment is needed to allow the nanosensors to enter inside the cell

Inverse spectral problems for Dirac operators with summable matrix-valued potentials

We consider the direct and inverse spectral problems for Dirac operators on $(0,1)$ with matrix-valued potentials whose entries belong to $L_p(0,1)$, $p\in[1,\infty)$. We give a complete description of the spectral data (eigenvalues and suitably introduced norming matrices) for the operators under consideration and suggest a method for reconstructing the potential from the corresponding spectral data.Comment: 32 page

Сучасні підходи до лікування травматичної хвороби головного мозку (травматичної енцефалопатії)

Наведено сучасні уявлення щодо лікування травматичної хвороби головного мозку (травматичної енцефалопатії). Особливу увагу приділено екстракорпоральним методам лікування травматичної енцефалопатії.</jats:p

INTENSITY OF LIPID PEROXIDATION IN THE MICROSOMAL FRACTION OF RAT LIVER UNDER THE EFFECTS OF SODIUM BENZOATE AND ASCORBIC ACID

The effect of food preservatives on the state of free radical processes in rat liver cells are studied in the paper. The changes of lipid peroxidation (LPO) products (the level of primary products – diene conjugates (DK), secondary products – ketodienes and conjugated trienes (KD + CT), TBA-active products and final products – Schiff bases) in the microsomal fraction of rat liver were studied under conditions of introduction into the body of sodium benzoate and ascorbic acid. Animals were divided into four groups: group I – intact animals (control); group II – rats, which were administra-tion of ascorbic acid; group III – rats, which were administration of sodium benzoate; group IV – rats, which were administration of sodium benzoate 30 min before the introduction of ascorbic acid. Sodium benzoate and ascorbic acid were administered per os daily for 21 days. Euthanasia of animals was performed under light ether anesthesia on the 21st day after the administration of sodium benzoate and ascorbic acid. It was established that the daily introduction of sodium benzoate into the body leads to the initiation of LPO in the microsomal fraction of the liver, which is evidenced by an increase in the levels of primary, secondary and final prod-ucts of LPO compared to the indicators of intact animals. Sodium benzoate exhibits a higher pro-oxidant effect when combined with ascorbic acid. It has been shown that under the conditions of administration of sodium benzoate and ascorbic acid, LPO processes are intensified, as the levels of DK, KD + ST, TBK-active products and Schiff bases significantly exceed the indicators of the group of ani-mals that were administered only sodium benzoate.</jats:p

ENZYMATIC ACTIVITY OF THE DEHYDROGENASE AND OXIDASE FORMS OF XANTHINOXIDASE IN THE LIVER OF RATS UNDER THE CONDITIONS OF ω-3 POLYNUNSATURATED FATTY ACIDS AND RETINOIDS

The effect of retinoid different doses in a complex with ω-3 polyunsaturated fatty acids (PUFA) on the enzyme activity of dehydrogenase (D-form – xanthine:NAD+-oxidoreductase, EC 1.17.1.4)) and oxidase (O-form – xanthine: oxygen oxidoreductase, EC 1.17.3.2) isoforms of xanthine oxidase and the generation of superoxide radical (O2•-) in the liver cytosolic fraction of rat are studied. Animals were divided into four groups: I – intact animals (control); II – rats injected with ω-3 PUFA at a dose of 120 mg/kg of animal weight; III, IV, V – animals administered retinol acetate at a dose of 60 IU/kg, 600 IU/kg, 1800 IU/kg, respectively; VI, VII, VIII – rats that were injected with a complex of ω-3 PUFAs and retinol acetate in doses of 60 IU/kg, 600 IU/kg, and 1800 IU/kg, respectively. Euthanasia of animals was performed under light ether anesthesia on the 14th day after the start of drug administration. It was established in-crease in total xanthine oxidase activity by 2.5 and 2.7 times compared to the values of the group of intact animals under the administering of a therapeutic dose of 600 IU/kg of retinol acetate and a maximum daily dose of 1800 IU/kg, respectively. The combined use of ω-3 PUFA and retinol acetate in doses of 600 IU/kg and 1800 IU/kg led to a decrease in the total xanthine oxidase activity in comparison with the values of animals that were administered the retinol acetate preparation separately in the corresponding doses. It is shown that the increase in the total activity of xanthine oxidase occurs due to the increase in the activity level of its O-form with a simultaneous decrease in the activity of the D-form. An increase of O-form xanthine oxidase activity in the liver cytosolic fraction the occurs in parallel with an increase in the rate of O2•- formation. This indicates the participation of this isoform in the induction of redox imbalance in the cytosol of liver cells under the influence of high doses of retinol acetate. The use of ω-3 PUFA along with retinol acetate contributes to the studied indicators normalization.</jats:p

Functional activity of NADH-dependent reductase system in liver and guerin’s carcinoma microsomal fraction in rats exposed to preliminary irradiation

The activity of liver and Guerin’s carcinoma microsomal NADH-dependent reductase system has been investigated in tumor-bearing rats exposed to preliminary irradiation. Preliminary irradiation of rats (before transplantation of Guerin’s carcinoma) resulted in the decrease of NADH-cytochrome b reductase activity and contents of cytochrome b in the Guerin’s carcinoma microsomal fraction in the logarithmic phases of oncogenesis compared with the non-irradiated tumor-bearing rats. The effect of irradiation preceding transplantation of the tumor to rats results in the increase of enzymatic activities of liver microsomal NADH-cytochrome b reductase in the latent and logarithmic phases of tumor growth as compared with non-irradiated tumor-bearing rats. At the same time the contents of cytochrome b decreases. During longer periods after irradiation the investigated parameters approached to those in the group of non-irradiated tumor-bearing animals.</jats:p

Invariant Schrödinger Operators with Point Interactions at the Vertices of a Regular Polyhedron

[No abstract available