α1-Antitrypsin and serum albumin mRNA accumulation in normal, acute phase and ZZ human liver

Abstractα1-Antitrypsin and albumin mRNA levels of 4 human livers were assessed using a newly sequenced cDNA clone of the carboxyterminal third of α1-antitrypsin and a previously cloned albumin cDNA sequence. The relative concentration of α1-antitrypsin mRNA was the same in poly(A)-containing RNA isolated from acute phase (MM) and α1-antitrypsin deficient (ZZ) individuals. In the acute phase liver relative to the normal (MM) liver, total RNA extracts showed a marked decrease in albumin mRNA concentration but no increase in α1-antitrypsin mRNA. The ZZ liver showed decreased total and poly(A)-containing RNA content but the same proportion of α-antitrypsin to albumin mRNA as in the normal (MM) liver. This supports other evidence that ZZ α1-antitrypsin deficiency is due to a defect in polypeptide processing (secretion) rather than a deficiency in mRNA accumulation