Laboratory observations of permeability enhancement by fluid pressure oscillation of in situ fractured rock

We report on laboratory experiments designed to investigate the influence of pore pressure oscillations on the effective permeability of fractured rock. Berea sandstone samples were fractured in situ under triaxial stresses of tens of megapascals, and deionized water was forced through the incipient fracture under conditions of steady and oscillating pore pressure. We find that short-term pore pressure oscillations induce long-term transient increases in effective permeability of the fractured samples. The magnitude of the effective permeability enhancements scales with the amplitude of pore pressure oscillations, and changes persist well after the stress perturbation. The maximum value of effective permeability enhancement is 5 × 10^(−16) m^2 with a background permeability of 1 × 10^(−15) m^2; that is, the maximum enhanced permeability is 1.5 × 10^(−15) m^2. We evaluate poroelastic effects and show that hydraulic storage release does not explain our observations. Effective permeability recovery following dynamic oscillations occurs as the inverse square root of time. The recovery indicates that a reversible mechanism, such as clogging/unclogging of fractures, as opposed to an irreversible one, like microfracturing, is responsible for the transient effective permeability increase. Our work suggests the feasibility of dynamically controlling the effective permeability of fractured systems. The result has consequences for models of earthquake triggering and permeability enhancement in fault zones due to dynamic shaking from near and distant earthquakes

Xray Tomographic Microscopic Studies a Resin Embedded Paint Sample from a Mechanical Failing Area in the Floor Tiles of The Art of Painting by Johannes Vermeer (1632-1675)

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 - August 5, 201

Diffraction dissociation in proton-proton collisions at s\sqrt{s} = 0.9 TeV, 2.76 TeV and 7 TeV with ALICE at the LHC

The relative rates of single- and double- diffractive processes were measured with the ALICE detector by studying properties of gaps in the pseudorapidity distribution of particles produced in proton-proton collisions at $\sqrt{s}$ = 0.9 TeV, 2.76 TeV and 7 TeV. ALICE triggering efficiencies are determined for various classes of events, using a detector simulation validated with data on inclusive particle production. Cross-sections are determined using van der Meer scans to measure beam properties and obtain a measurement of the luminosity

The mechanisms of spatial and temporal earthquake clustering

The number of earthquakes as a function of magnitude decays as a power law. This trend is usually justified using spring-block models, where slips with the appropriate global statistics have been numerically observed. However, prominent spatial and temporal clustering features of earthquakes are not reproduced by this kind of modeling. We show that when a spring-block model is complemented with a mechanism allowing for structural relaxation, realistic earthquake patterns are obtained. The proposed model does not need to include a phenomenological velocity weakening friction law, as traditional spring-block models do, since this behavior is effectively induced by the relaxational mechanism as well. In this way, the model provides also a simple microscopic basis for the widely used phenomenological rate-and-state equations of rock friction.Comment: 7 pages, 10 figures, comments welcom

Mechanisms for slow strengthening in granular materials

Several mechanisms cause a granular material to strengthen over time at low applied stress. The strength is determined from the maximum frictional force F_max experienced by a shearing plate in contact with wet or dry granular material after the layer has been at rest for a waiting time \tau. The layer strength increases roughly logarithmically with \tau -only- if a shear stress is applied during the waiting time. The mechanisms of strengthening are investigated by sensitive displacement measurements and by imaging of particle motion in the shear zone. Granular matter can strengthen due to a slow shift in the particle arrangement under shear stress. Humidity also leads to strengthening, but is found not to be its sole cause. In addition to these time dependent effects, the static friction coefficient can also be increased by compaction of the granular material under some circumstances, and by cycling of the applied shear stress.Comment: 21 pages, 11 figures, submitted to Phys. Rev.

Caffeine Inhibits EGF-Stimulated Trophoblast Cell Motility through the Inhibition of mTORC2 and Akt.

Impaired trophoblast invasion is associated with pregnancy disorders such as early pregnancy loss and preeclampsia. There is evidence to suggest that the consumption of caffeine during pregnancy may increase the risk of pregnancy loss; however, little is known about the direct effect of caffeine on normal trophoblast biology. Our objectives were to examine the effect of caffeine on trophoblast migration and motility after stimulation with epidermal growth factor (EGF) and to investigate the intracellular signaling pathways involved in this process. Primary first-trimester extravillous trophoblasts (EVT) and the EVT-derived cell line SGHPL-4 were used to study the effect of caffeine on EGF-stimulated cellular motility using time-lapse microscopy. SGHPL-4 cells were further used to study the effect of caffeine and cAMP on EGF-stimulated invasion of fibrin gels. The influence of caffeine and cAMP on EGF-stimulated intracellular signaling pathways leading to the activation of Akt were investigated by Western blot analysis. Caffeine inhibits both EGF-stimulated primary EVT and SGHPL-4 cell motility. EGF stimulation activates phosphatidylinositol 3-kinase, and Akt and caffeine inhibit this activation. Although cAMP inhibits both motility and invasion, it does not inhibit the activation of Akt, indicating that the effects of caffeine seen in this study are independent of cAMP. Further investigation indicated a role for mammalian target of rapamycin complex 2 (mTORC2) as a target for the inhibitory effect of caffeine. In conclusion, we demonstrate that caffeine inhibits EGF-stimulated trophoblast invasion and motility in vitro and so could adversely influence trophoblast biology in vivo

Scanning Electron Microscopy and Synchrotron Radiation X-Ray Tomographic Microscopy of 330 Million Year Old Charcoalified Seed Fern Fertile Organs

Abundant charcoalified seed fern (pteridosperm) pollen organs and ovules have been recovered from Late Viséan (Mississippian 330 Ma) limestones from Kingswood, Fife, Scotland. To overcome limitations of data collection from these tiny, sometimes unique, fossils, we have combined low vacuum scanning electron microscopy on uncoated specimens with backscatter detector and synchrotron radiation X-ray tomographic microscopy utilizing the Materials Science and TOMCAT beamlines at the Swiss Light Source of the Paul Scherrer Institut. In combination these techniques improve upon traditional cellulose acetate peel sectioning because they enable study of external morphology and internal anatomy in multiple planes of section on a single specimen that is retained intact. The pollen organ Melissiotheca shows a basal parenchymatous cushion bearing more than 100 sporangia on the distal face. Digital sections show the occurrence of pollen in some sporangia. The described ovule is new and has eight integumentary lobes that are covered in spirally arranged glandular hairs. Virtual longitudinal sections reveal the lobes are free above the pollen chamber. Results are applied in taxonomy and will subsequently contribute to our understanding of the former diversity and evolution of ovules, seeds, and pollen organs in the seed ferns, the first seed-bearing plants to conquer the lan