Dimensão pessoal do processo de trabalho para enfermeiras de Unidades de Terapia Intensiva

OBJETIVO: Compreender os aspectos da dimensão pessoal do processo de trabalho para enfermeiras que atuam em Unidades de Terapia Intensiva (UTI) de um hospital universitário do interior do Estado de São Paulo. MÉTODOS: Estudo de abordagem qualitativa utilizando o método fenomenológico. Foram entrevistadas 12 enfermeiras que trabalhavam nas UTI de adulto, pediátrica e coronariana dessa instituição hospitalar e para análise dos relatos, utilizou-se o referencial da estrutura do fenômeno situado. RESULTADOS: A análise dos relatos permitiu identificar a categoria: dimensão pessoal do trabalho na UTI, com os temas - trabalho gratificante, trabalho desgastante e (des) valorização do trabalho. CONCLUSÃO: Os resultados do estudo revelam que as enfermeiras das UTI selecionadas identificam-se com o trabalho, porém vivenciam situações desmotivadoras decorrentes da elevada carga de trabalho, que dificulta o desenvolvimento do processo de trabalho e compromete a qualidade do cuidado.OBJETIVO: Comprender los aspectos de la dimensión personal del proceso de trabajo para enfermeras que actúan en Unidades de Cuidados Intensivos (UCI) de un hospital universitario del interior del Estado de Sao Paulo. MÉTODOS: Se trata de un estudio con abordaje cualitativo en el que se usó el método fenomenológico. Se entrevistaron a 12 enfermeras que trabajaban en las UCI de adulto, pediátrica y coronaria de esa institución hospitalaria y para el análisis de los relatos, se consideró el referencial de la estructura del fenómeno situado. RESULTADOS: El análisis de los relatos permitió identificar la categoría: dimensión personal del trabajo en la UCI, con los temas - trabajo gratificante, trabajo desgastante y (des) valorización del trabajo. CONCLUSIÓN: Los resultados del estudio revelan que las enfermeras de las UCI seleccionadas se identifican con el trabajo, no obstante vivencian situaciones desmotivadoras como consecuencia de la elevada carga de trabajo, que dificulta el desarrollo del proceso de trabajo y compromete la calidad del cuidado.OBJECTIVE: To understand the personal dimension of the work process for nurses working in intensive care units (ICU) of a university hospital in the state of São Paulo. METHODS: A qualitative study using the phenomenological method. We interviewed 12 nurses working in adult, pediatric and coronary ICUs in this hospital; to analyze results, we used the phenomenonogical method. RESULTS: The analysis of the results identified the category, personal dimension of ICU work, which included the themes, "rewarding work", "exhausting work", and, "lack of appreciation of the work". CONCLUSION: The results of this study revealed that the ICU nurses interviewed identified with their work, but that they experience discouraging situations resulting from high workload, which hinders the development of the work process and compromises the quality of care

Endothelial Differentiation of Human Stem Cells Seeded onto Electrospun Polyhydroxybutyrate/Polyhydroxybutyrate-Co-Hydroxyvalerate Fiber Mesh

Tissue engineering is based on the association of cultured cells with structural matrices and the incorporation of signaling molecules for inducing tissue regeneration. Despite its enormous potential, tissue engineering faces a major challenge concerning the maintenance of cell viability after the implantation of the constructs. The lack of a functional vasculature within the implant compromises the delivery of nutrients to and removal of metabolites from the cells, which can lead to implant failure. In this sense, our investigation aims to develop a new strategy for enhancing vascularization in tissue engineering constructs. This study's aim was to establish a culture of human adipose tissue-derived stem cells (hASCs) to evaluate the biocompatibility of electrospun fiber mesh made of polyhydroxybutyrate (PHB) and its copolymer poly-3-hydroxybutyrate-co-3-hydroxyvalerate (PHB-HV) and to promote the differentiation of hASCs into the endothelial lineage. Fiber mesh was produced by blending 30% PHB with 70% PHB-HV and its physical characterization was conducted using scanning electron microscopy analysis (SEM). Using electrospinning, fiber mesh was obtained with diameters ranging 300 nm to 1.3 µm. To assess the biological performance, hASCs were extracted, cultured, characterized by flow cytometry, expanded and seeded onto electrospun PHB/PHB-HV fiber mesh. Various aspects of the cells were analyzed in vitro using SEM, MTT assay and Calcein-AM staining. The in vitro evaluation demonstrated good adhesion and a normal morphology of the hASCs. After 7, 14 and 21 days of seeding hASCs onto electrospun PHB/PHB-HV fiber mesh, the cells remained viable and proliferative. Moreover, when cultured with endothelial differentiation medium (i.e., medium containing VEGF and bFGF), the hASCs expressed endothelial markers such as VE-Cadherin and the vWF factor. Therefore, the electrospun PHB/PHB-HV fiber mesh appears to be a suitable material that can be used in combination with endothelial-differentiated cells to improve vascularization in engineered bone tissues

Endoparasites of horses from the Formiga city, located in center-west region of the state of Minas Gerais, Brazil

With the aim of studying the endoparasite fauna of horses from the Formiga city, located in center-west region of the state of Minas Gerais, 25 animals that were naturally infected with helminths were evaluated. By means of parasitological necropsies, different endoparasites were found. The subfamily Cyathostominae presented the highest incidence, followed by Trichostrongylus axei, Oxyuris equi, Triodontophorus serratus, Strongyloides westeri, Strongylus edentatus, Habronema muscae, Parascaris equorum, Probstmayria vivipara, Strongylus vulgaris, Gasterophilus nasalis, Anoplocephala magna and Anoplocephala perfoliata. In the present study, if the species Probstmayria vivipara was not considered in the prevalence, the frequency of Cyathostominae was equivalent to 94.85%. The results obtained in this study allowed us to detect and identify different species of helminths in horses, and confirmed the high incidence of nematodes belonging to the subfamily Cyathostominae in the center-west region of Minas Gerais.Com o objetivo de estudar a fauna de endoparasitas de equinos da Região Centro-Oeste do Estado de Minas Gerais, 25 animais naturalmente infectados por helmintos foram avaliados. Por meio de necropsias parasitológicas, diferentes endoparasitas foram identificados. A sub - família Cyathostominae apresentou maior incidência, seguido por Trichostrongylus axei, Oxyuris equi, Triodontophorus serratus, Strongyloides westeri, Strongylus edentatus,Habronema muscae, Parascaris equorum, Probstmayria vivipara, Strongylus vulgaris, Gasterophilus nasalis,Anoplocephala magnae Anoplocephala perfoliata. No presente estudo, se não for considerada a espécieProbstmayria vivipara na prevalência, a frequência de Cyathostominae é equivalente a 94,85%. Os resultados obtidos neste estudo, permitiu detectar e identificar diferentes espécies de helmintos em equinos, bem como confirmar a elevada incidência de nematódeos pertencentes à sub-família Cyathostominae na Região Centro-Oeste de Minas Gerais.State Univ Sao Paulo UNESP, Fac Agr &Vet Sci, Ctr Res Anim Hlth CPPAR, BR-14884900 Jaboticabal, SP, BrazilState Univ Sao Paulo UNESP, Fac Agr &Vet Sci, Ctr Res Anim Hlth CPPAR, BR-14884900 Jaboticabal, SP, Brazi