Detection of human neutrophil elastase (HNE) on wound dressings as marker of inflammation

Chronic wound fluids have elevated concentration of human neutrophil elastase (HNE) which can be used as inflammation/infection marker. Our goal is to develop functional materials for fast diagnosis of wound inflammation/infection by using HNE as a specific marker. For that, fluorogenic peptides with a HNE-specific cleavage sequence were incorporated into traditional textile dressings, to allow real-time detection of the wound status. Two different fluorogenic approaches were studied in terms of intensity of the signal generated upon HNE addition: a fluorophore 7-amino-4-trifluormethylcoumarin (AFC) conjugated to a HNE-specific peptide and two fluorophore/quencher pairs (FAM/Dabcyl and EDANS/Dabcyl) coupled to a similar peptide as a Förster resonance energy transfer (FRET) strategy. Also, two immobilization methods were tested: sonochemistry immobilization onto a cotton bandage and glutaraldehyde (GTA)-assisted chemical crosslinking onto a polyamide dressing. The immobilized fluorogenic AFC peptide showed an intense fluorescence emission in the presence of HNE. HNE also induced an enhanced fluorescent signal with the EDANS/Dabcyl FRET peptide which showed to be a more sensitive and effective strategy than the AFC peptide. However, its chemical immobilization onto the polyamide dressing greatly decreased its detection, mainly due to the more difficult access of the enzyme to the cleavage sequence of the immobilized peptide. After optimization of the in situ immobilization, it will be possible to use these fluorescence-functionalized dressings for an effective and specific monitoring of chronic wounds by simply using a portable ultraviolet (UV) light source. We envision that the development of this point-of-care medical device for wound control will have a great impact on patients life quality and reduction of costs on health care system.This study was funded by the European project InFact-Functional materials for fast diagnosis of wound infection (FP7-NMP-2013-SME-7-grant agreement no. 604278). The work done at Centre of Biological Engineering (CEB) was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte 2020-Programa Operacional Regional do Norte

Superhydrophilic self-cleaning cotton fabric with enhanced antibacterial and UV protection properties

202501 bcchVersion of RecordSelf-fundedPublishedSpringer Nature (2024)T

Antibacterial Superhydrophobic Cotton Fabric with Photothermal, Self-Cleaning, and Ultraviolet Protection Functionalities

Cotton fabrics with superhydrophobic, antibacterial, UV protection, and photothermal properties were developed using Ag/PDMS coatings, and the role of coating formulations on the obtained functionalities was studied. Specific attention was paid to understanding the relationships between the fabrics’ superhydrophobicity and antibacterial activity against Escherichia coli (E. coli) bacteria. UV protection performance of Ag/PDMS coatings was thoroughly evaluated based on the variation of UV transmission rate through coated fabrics and photoinduced chemiluminescence spectra. Moreover, the effect of silver nanoparticles (Ag NPs) and PDMS on developing a photothermal effect on fabrics was discussed. It was found that the content of Ag NPs and PDMS played critical roles in determining the water contact angle (WCA) on modified fabrics. The largest WCA was 171.31°, which was durable even after numerous accelerated wash cycles and abrasions. Antibacterial activity of fabrics showed the positive effect of pure PDMS in bacterial growth inhibition. Moreover, it was found that the antibacterial performance was greatly affected by the content of Ag NPs loaded on fabrics rather than their superhydrophobic status. Moreover, increasing the content of Ag NPs boosted the UV protection level of fabrics, improved fabrics photostability, and reduced the UV transmission rate through fabrics. Testing the photothermal effect confirmed that the content of Ag NPs and PDMS both played prominent roles, where Ag acted as a photothermal agent and PDMS determined the NIR reflection rate from the coated surface. The modified fabrics were characterized using TGA, SEM, FTIR, and XRD techniques, and it was confirmed that using a higher amount of PDMS increased the amount of Ag NPs deposition on fabrics

Nonlinear deformation and localized failure of bacterial streamers in creeping flows

We investigate the failure of bacterial floc mediated streamers in a microfluidic device in a creeping flow regime using both experimental observations and analytical modeling. The quantification of streamer deformation and failure behavior is possible due to the use of 200 nm fluorescent polystyrene beads which firmly embed in the extracellular polymeric substance (EPS) and act as tracers. The streamers, which form soon after the commencement of flow begin to deviate from an apparently quiescent fully formed state in spite of steady background flow and limited mass accretion indicating significant mechanical nonlinearity. This nonlinear behavior shows distinct phases of deformation with mutually different characteristic times and comes to an end with a distinct localized failure of the streamer far from the walls. We investigate this deformation and failure behavior for two separate bacterial strains and develop a simplified but nonlinear analytical model describing the experimentally observed instability phenomena assuming a necking route to instability. Our model leads to a power law relation between the critical strain at failure and the fluid velocity scale exhibiting excellent qualitative and quantitative agreeing with the experimental rupture behavior