MOTIVASI DAN PERSEPSI MENONTON PROGRAM DIALOG INTERAKTIF (Studi Deskriptif Kualitatif Motivasi dan Persepsi Menonton Acara Debat Partai di TvOne di Kalangan Mahasiswa Komunikasi Non Reguler 2006 FISIP UNS Surakarta)

Television as a media accepted by society give much benefit in entertainment and information. People needs of information and entertainment had growth in recent year, so it makes television as a media that needed by every family. The existence of mass media especially will support the creation of information society, where to become an information society, people disposed information. For that reason, the television world race together to gives a lot of information to people. The competition between television programs give profit to the TV viewers, where viewers have a habit to select interactive dialogue programs that can be satisfied them. The viewer’s decision to select and enjoy an interactive dialogue program from TV station will be influenced by their motivation. Is the interactive dialogue programs could fulfill their information needs. In fact, it is impossible for viewers to consume (without certain technology) two programs in the same slots. This research purposed to know the motivation and perception of college student in watching party debate on TvOne, which those program perform debate between parties in general election 2009. From all of the informants, TV media can be one thing which supports them in getting various information especially the information of general election in 2009. Where each TV station broadcasts various programs from news until interactive dialogue programs that discuss about general election in 2009. The interactive dialogue programs have become something that gives various information, especially about general election in 2009. The interactive dialogue programs that broadcasted by TV one is party debate, where this programs be one of interesting interactive dialogue programs discuss about general election in 2009 which attracting to be watched, because it is not only gives information about the election but also gives information about the profile of each party in addressing an opinion from its rival party in general election or from the society

THE POTENTIAL GENOTYPES WITH HIGH TIMBER VOLUME FROM SIX SPECIES IN THE 1995 RRIM HEVEA GERMPLASM COLLECTION

The Hevea germplasm expedition by Rubber Research Institute of Malaysia in 1995 collected six Hevea species (H. brasiliensis, H. spruceana, H. guainensis, H. nitida, H. benthamiana and H. pauciflora) from the state of Amazonas of Brazil which are being conserved ex-situ in Rantau Panjang Reserve Forest, Batu Arang, Selangor. This expedition was a worthwhile scientific activity to safeguard valuable genetic resources for the future crop improvement of the Hevea species. However, after twenty years of establishment in the field, the evaluation of these materials is still rather limited and needs to be continued in order to explore the genetic potential of this germplasm. This paper highlights the evaluation of these genotypes for their potential as timber cultivars. A total of 5,789 individuals of the six Hevea species were evaluated in terms of girth, girth increment, bark thickness, annual bark increment, clear bole volume and total wood volume. At the age of 20 years, the highest clear bole volume observed for H. brasiliensis was 1.84 m3 with straight, smooth and rounded trunk. As for the other Hevea species, the highest clear bole volume for H. spruceana was 1.19 m3, followed by H. guainensis with 1.10 m3, H. nitida with 0.98 m3, H. pauciflora with 0.90 m3 and H. benthamiana with 0.89 m3. The identified genotypes have the potential to be used as a resource for timber to fulfil the demand of the rubber wood industry. However, further evaluation of these genotypes in high-density planting and under different environment is essential to verify their performance before a recommendation for wide scale planting could be made.Keywords: Genetic resources, Hevea species, the 1995 RRIM Hevea germplas

Implementasi Perangkat Peremajaan dan Perawatan Alat Berat Container Crane

Proses perbaikan dan perencanaan perawatan pada container crane yang di pelabuhan sering dianggap kurang efektif karena kurangnya perencanaan dalam pengelolaan yang berpengaruh dalam penentuan jadwal perawatan. Terminal Nilam PELINDO III Surabaya merupakan perusahaan yang bergerak dalam bidang jasa bongkar muat container crane. Proses bongkar muat ditentukan oleh beberapa faktor, seperti banyaknya container dan berat muatan yang dikelola, maupun kualitas dan kuantitas alat yang digunakan. Proses secara terus-menerus tersebut mempengaruhi usia container crane. Kurang tepatnya penentuan perawatan bisa berdampak pada performa container crane. Untuk menjawab permasalahan ini, dibutuhkan perencanaan sehingga mampu menentukan waktu perawatan yang tepat dalam kurun waktu tertentu. Pada penelitian ini, kami mengimplementasikan perencanaan perawatan dengan implementasi aplikasi berbasis web menggunakan bahasa pemrograman PHP dan database MariaDB. Kami menggunakan data operasional pada PT. BIMA PELINDO III Surabaya yang meliputi produksi terjadwal, penjagaan terjadwal, aktivitas pemeliharaan dan perkiraan terjadwal. Kami menentukan variabel input berdasarkan data operasional, yang dilanjutkan dengan permodelan metode perencanaan. Berdasarkan variabel input tersebut, akan diperoleh variabel output berupa kluster ringan dan berat, dengan setiap class diberi pembobotan prediksi perencanaan perawatan container crane yang ditampilkan ke aplikasi. Hasil dari aplikasi perencanaan perawatan ini diharapkan dapat menentukan perkiraan aktivitas pemeliharaan, perawatan terjadwal agar berjalan dengan baik, pemakaian bahan bakar dan sumber daya operasional container crane yang lain menjadi lebih efisien.</jats:p

Klasifikasi Jenis Pemeliharaan dan Perawatan Container Crane menggunakan Algoritma Machine Learning

Pada industri jasa pelayanan peti kemas, Terminal Nilam merupakan pelanggan dari PT. BIMA, yang secara khusus bergerak dibidang jasa perbaikan dan perawatan alat berat. Terminal ini menjadi sentral tempat untuk melakukan aktifitas bongkar muat peti kemas domestik yang memiliki empat buah container crane untuk melayani dua kapal. Proses perawatan alat berat seperti container crane yang selama ini beroperasi, agaknya kurang memperhatikan data pengelompokkan atau klasifikasi jenis perawatan yang dibutuhkan oleh alat berat tersebut. Di kemudian hari, alat berat dapat menunjukkan kinerja yang tidak maksimal bahkan dapat berujung pada kecelakaan kerja. Selain itu, kelalaian perawatan container crane juga dapat menyebabkan pembengkakan biaya perawatan lanjut. Target produksi bongkar muat dapat berkurang dan juga keterlambatan jadwal kapal sandar sangat mungkin terjadi. Metode pembelajaran menggunakan mesin atau biasa disebut dengan Machine Learning (ML), dengan mudah dapat melenyapkan kemungkinan-kemungkinan tersebut. ML dalam penelitian ini, kami rancang agar bekerja dengan mengidentifikasi lalu mengelompokkan jenis perawatan container crane yang sesuai, yaitu ringan atau berat. Metode ML yang pilih untuk digunakan dalam penelitian ini yaitu Random Forest, Support Vector Machine, k-Nearest Neighbor, Naïve Bayes, Logistic Regression, J48, dan Decision Tree. Penelitian ini menunjukkan keberhasilan ML model tree dalam melakukan pembelajaran jenis data perawatan container crane (numerik dan kategoris), dengan J48 menunjukkan performa terbaik dengan nilai akurasi dan nilai ROC-AUC mencapai 99,1%. Pertimbangan klasifikasi kami lakukan dengan mengacu kepada tanggal terakhir perawatan, hour meter, breakdown, shutdown, dan sparepart.</jats:p

Skin Bacteria Mediate Glycerol Fermentation to Produce Electricity and Resist UV-B

Bacteria that use electron transport proteins in the membrane to produce electricity in the gut microbiome have been identified recently. However, the identification of electrogenic bacteria in the skin microbiome is almost completely unexplored. Using a ferric iron-based ferrozine assay, we have identified the skin Staphylococcus epidermidis (S. epidermidis) as an electrogenic bacterial strain. Glycerol fermentation was essential for the electricity production of S. epidermidis since the inhibition of fermentation by 5-methyl furfural (5-MF) significantly diminished the bacterial electricity measured by voltage changes in a microbial fuel cell (MFC). A small-scale chamber with both anode and cathode was fabricated in order to study the effect of ultraviolet-B (UV-B) on electricity production and bacterial resistance to UV-B. Although UV-B lowered bacterial electricity, a prolonged incubation of S. epidermidis in the presence of glycerol promoted fermentation and elicited higher electricity to suppress the effect of UV-B. Furthermore, the addition of glycerol into S. epidermidis enhanced bacterial resistance to UV-B. Electricity produced by human skin commensal bacteria may be used as a dynamic biomarker to reflect the UV radiation in real-time.</jats:p

Skin Bacteria Mediate Glycerol Fermentation to Produce Electricity and Resist UV-B

Bacteria that use electron transport proteins in the membrane to produce electricity in the gut microbiome have been identified recently. However, the identification of electrogenic bacteria in the skin microbiome is almost completely unexplored. Using a ferric iron-based ferrozine assay, we have identified the skin Staphylococcus epidermidis (S. epidermidis) as an electrogenic bacterial strain. Glycerol fermentation was essential for the electricity production of S. epidermidis since the inhibition of fermentation by 5-methyl furfural (5-MF) significantly diminished the bacterial electricity measured by voltage changes in a microbial fuel cell (MFC). A small-scale chamber with both anode and cathode was fabricated in order to study the effect of ultraviolet-B (UV-B) on electricity production and bacterial resistance to UV-B. Although UV-B lowered bacterial electricity, a prolonged incubation of S. epidermidis in the presence of glycerol promoted fermentation and elicited higher electricity to suppress the effect of UV-B. Furthermore, the addition of glycerol into S. epidermidis enhanced bacterial resistance to UV-B. Electricity produced by human skin commensal bacteria may be used as a dynamic biomarker to reflect the UV radiation in real-time

Repurposing INCI-registered compounds as skin prebiotics for probiotic Staphylococcus epidermidis against UV-B

AbstractRepurposing existing compounds for new indications may facilitate the discovery of skin prebiotics which have not been well defined. Four compounds that have been registered by the International Nomenclature of Cosmetic Ingredients (INCI) were included to study their abilities to induce the fermentation of Staphylococcusepidermidis (S. epidermidis), a bacterial species abundant in the human skin. Liquid coco-caprylate/caprate (LCC), originally used as an emollient, effectively initiated the fermentation of S. epidermidis ATCC 12228, produced short-chain fatty acids (SCFAs), and provoked robust electricity. Application of LCC plus electrogenic S. epidermidis ATCC 12228 on mouse skin significantly reduced ultraviolet B (UV-B)-induced injuries which were evaluated by the formation of 4-hydroxynonenal (4-HNE), cyclobutane pyrimidine dimers (CPD), and skin lesions. A S. epidermidis S2 isolate with low expressions of genes encoding pyruvate dehydrogenase (pdh), and phosphate acetyltransferase (pta) was found to be poorly electrogenic. The protective action of electrogenic S. epidermidis against UV-B-induced skin injuries was considerably suppressed when mouse skin was applied with LCC in combination with a poorly electrogenic S. epidermidis S2 isolate. Exploring new indication of LCC for promoting S. epidermidis against UV-B provided an example of repurposing INCI-registered compounds as skin prebiotics.</jats:p

Electricity-producing Staphylococcus epidermidis counteracts Cutibacterium acnes

AbstractStaphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) activity of electrogenic S. epidermidis was assessed in vitro and in vivo. The voltage change (~ 4.4 mV) reached a peak 60 min after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anodes. The electricity produced by S. epidermidis caused significant growth attenuation and cell lysis of C. acnes. Intradermal injection of C. acnes and S. epidermidis plus PEG-8 Laurate into the mouse ear considerably suppressed the growth of C. acnes. This suppressive effect was noticeably reversed when cyclophilin A of S. epidermidis was inhibited, indicating the essential role of cyclophilin A in electricity production of S. epidermidis against C. acnes. In summary, we demonstrate for the first time that skin S. epidermidis, in the presence of PEG-8 Laurate, can mediate cyclophilin A to elicit an electrical current that has anti-C. acnes effects. Electricity generated by S. epidermidis may confer immediate innate immunity in acne lesions to rein in the overgrowth of C. acnes at the onset of acne vulgaris.</jats:p

Electricity-Producing Staphylococcus Epidermidis Counteracts Cutibacterium Acnes

Abstract Staphylococcus epidermidis (S. epidermidis) ATCC 12228 was incubated with 2% polyethylene glycol (PEG)-8 Laurate to yield electricity which was measured by a voltage difference between electrodes. Production of electron was validated by a Ferrozine assay. The anti-Cutibacterium acnes (C. acnes) activity of electrogenic S. epidermidis was assessed in vitro and in vivo. The voltage change (~ 4.4 mV) reached a peak 60 minutes after pipetting S. epidermidis plus 2% PEG-8 Laurate onto anodes. The electricity produced by S. epidermidis caused significant growth attenuation and cell lysis of C. acnes. Intradermal injection of C. acnes and S. epidermidis plus PEG-8 Laurate into the mouse ear considerably suppressed the growth of C. acnes. This suppressive effect was noticeably reversed when cyclophilin A of S. epidermidis was inhibited, indicating the essential role of cyclophilin A in electricity production of S. epidermidis against C. acnes. In summary, we demonstrate for the first time that skin S. epidermidis, in the presence of PEG-8 Laurate, can mediate cyclophilin A to elicit an electrical current that has anti-C. acnes effects. Electricity generated by S. epidermidis may confer immediate innate immunity in acne lesions to rein in the overgrowth of C. acnes at the onset of acne vulgaris.</jats:p

Leuconostoc mesenteroides mediates an electrogenic pathway to attenuate the accumulation of abdominal fat mass induced by high fat diet

AbstractAlthough several electrogenic bacteria have been identified, the physiological effect of electricity generated by bacteria on host health remains elusive. We found that probiotic Leuconostoc mesenteroides (L. mesenteroides) can metabolize linoleic acid to yield electricity via an intracellular cyclophilin A-dependent pathway. Inhibition of cyclophilin A significantly abolished bacterial electricity and lowered the adhesion of L. mesenteroides to the human gut epithelial cell line. Butyrate from L. mesenteroides in the presence of linoleic acid were detectable and mediated free fatty acid receptor 2 (Ffar2) to reduce the lipid contents in differentiating 3T3-L1 adipocytes. Oral administration of L. mesenteroides plus linoleic acid remarkably reduced high-fat-diet (HFD)-induced formation of 4-hydroxy-2-nonenal (4-HNE), a reactive oxygen species (ROS) biomarker, and decreased abdominal fat mass in mice. The reduction of 4-HNE and abdominal fat mass was reversed when cyclophilin A inhibitor-pretreated bacteria were administered to mice. Our studies present a novel mechanism of reducing abdominal fat mass by electrogenic L. mesenteroides which may yield electrons to enhance colonization and sustain high amounts of butyrate to limit ROS during adipocyte differentiation.</jats:p