10 research outputs found
Activated cyclophosphamide: An enzyme-mechanism-based suicide inactivator of DNA polymerase/3??5? exonuclease
No full textInsertion of specific bases during DNA synthesis past the oxidation-damaged base 8-oxodG
No full textA slipped-CAG DNA-binding small molecule induces trinucleotide-repeat contractions in vivo.
No full textSequential initiation of lagging and leading strand synthesis by two different polymerase complexes at the SV40 DNA replication origin
No full text
Identification and sequence characterization of a 1.3 Kb EcoRI repeat fragment that harbors a DNA repair site of rat pachytene spermatocytes
No full textSV40 DNA Replication
No full textJust as some of the pioneers of molecular biology turned to bacteriophages because of their relative simplicity (Cairns et al. 1966), investigations into the molecular biology of eukaryotes has often relied on studies of viruses. One of these viruses, Simian Virus 40, (SV40) has been the focus of many seminal studies. The virus, tumorigenic in nonpermissive rodent cells, was crucial in the early studies of oncogenic transformation. Because the virus is permissive in both simian and human cells, it has become a superb tool for investigating human cell biology. Biological questions as diverse as intracellular targeting, transcription, and transformation have all taken advantage of studies on SV40. Similarly, research on human DNA replication has benefited greatly by using the SV40 system as a tool. SV40 has the additional property that its DNA is assembled into chromatin in a way similar to human chromosomal DNA. Hence, the system can be used to study not only DNA replication but also the concomitant process of chromatin assembly
