Gelatinase activity of exoantigens from virulent and non-virulent isolates of Paracoccidioides brasiliensis

Differences in the occurrence of components with gelatinase activity were detected among four isolates of Paracoccidioides brasiliensis: Pb339 and Pb18 (highly virulent), and Pb265 and Pb18AV (very low virulence). Culture filtrates from these isolates were electrophoresed in substrate gels and tested for gelatinase activity. Pb339 showed three enzyme bands of apparent molecular masses: 43, 53 and 78 kDa; Pb18 had two bands, one at 59 kDa and another with molecular mass higher than 78 kDa. Isolate Pb18AV showed only one band at 78 kDa and Pb265 exhibited a component of molecular mass which failed to enter the separating gel.CENT PUBL HLTH LAB,MYCOL REFERENCE LAB,LONDON,ENGLANDESCOLA PAULISTA MED,DEPT MOLEC BIOL,SAO PAULO,SP,BRAZILESCOLA PAULISTA MED,DEPT MOLEC BIOL,SAO PAULO,SP,BRAZILWeb of Scienc

SPECIFIC RECOGNITION PATTERN OF IGM AND IGG ANTIBODIES PRODUCED IN THE COURSE OF EXPERIMENTAL PARACOCCIDIOIDOMYCOSIS

Specific IgM and IgG responses to Paracoccidioides brasiliensis produced in resistant and susceptible mice during experimental paracoccidioidomycosis were examined by the immunoblotting procedure. Sera from infected mice recognized 51 antigen bands with apparent molecular masses from 8 to 86 kD. Sixteen of these were defined as major antigen bands because of almost universal presence of antibodies to them, and their intense staining. All sera, including those from normal control mice, tested for both IgM and IgG antibody reacted with the major E antigen which appeared as a large diffuse band from 43 to 47 kD. Comparisons between resistant and susceptible mice showed some significant differences in IgM responses to many antigen bands. While IgG responses were quite similar for both strains, differences were apparent in the response to the antigens at 62 and 68 kD.UNIV SAO PAULO,INST CIENCIAS BIOMED,DEPT IMMUNOL,SAO PAULO,BRAZILESCOLA PAULISTA MED SCH,DISCIPLINA BIOL CELULAR,BR-04023 SAO PAULO,BRAZILESCOLA PAULISTA MED SCH,DISCIPLINA BIOL CELULAR,BR-04023 SAO PAULO,BRAZILWeb of Scienc