Cannabis through the looking glass: chemo- and enantio-selective separation of phytocannabinoids by enantioselective ultra high performance supercritical fluid chromatography

By using the Inverted Chirality Columns Approach (ICCA) we have developed an enantioselective UHPSFC method to determine the enantiomeric excess (ee) of (-)-Δ(9)-THC in medicinal marijuana (Bedrocan®). The ee was high (99.73%), but the concentration of the (+)-enantiomer (0.135%) was not negligible, and it is worth a systematic evaluation of bioactivity

An Update on the Lithium-Ion Cell Low-Earth-Orbit Verification Test Program

A Lithium-Ion Cell Low-Earth-Orbit Verification Test Program is being conducted by NASA Glenn Research Center to assess the performance of lithium-ion (Li-ion) cells over a wide range of low-Earth-orbit (LEO) conditions. The data generated will be used to build an empirical model for Li-ion batteries. The goal of the modeling will be to develop a tool to predict the performance and cycle life of Li-ion batteries operating at a specified set of mission conditions. Using this tool, mission planners will be able to design operation points of the battery system while factoring in mission requirements and the expected life and performance of the batteries. Test conditions for the program were selected via a statistical design of experiments to span a range of feasible operational conditions for LEO aerospace applications. The variables under evaluation are temperature, depth-of-discharge (DOD), and end-of-charge voltage (EOCV). The baseline matrix was formed by generating combinations from a set of three values for each variable. Temperature values are 10 C, 20 C and 30 C. Depth-of-discharge values are 20%, 30% and 40%. EOCV values are 3.85 V, 3.95 V, and 4.05 V. Test conditions for individual cells may vary slightly from the baseline test matrix depending upon the cell manufacturer s recommended operating conditions. Cells from each vendor are being evaluated at each of ten sets of test conditions. Cells from four cell manufacturers are undergoing life cycle tests. Life cycling on the first sets of cells began in September 2004. These cells consist of Saft 40 ampere-hour (Ah) cells and Lith ion 30 Ah cells. These cells have achieved over 10,000 cycles each, equivalent to about 20 months in LEO. In the past year, the test program has expanded to include the evaluation of Mine Safety Appliances (MSA) 50 Ah cells and ABSL battery modules. The MSA cells will begin life cycling in October 2006. The ABSL battery modules consist of commercial Sony hard carbon 18650 lithium-ion cells configured in series and parallel combinations to create nominal 14.4 volt, 3 Ah packs (4s-2p). These modules have accumulated approximately 3000 cycles. Results on the performance of the cells and modules will be presented in this paper. The life prediction and performance model for Li-ion cells in LEO will be built by analyzing the data statistically and performing regression analysis. Cells are being cycled to failure so that differences in performance trends that occur at different stages in the life of the cell can be observed and accurately modeled. Cell testing is being performed at the Naval Surface Warfare Center in Crane, IN

Neurobiology of social attachments

Many types of social attachments can be observed in nature. We discuss the neurobiology of two types (1) intraspecific (with a partner) and (2) parental (with the offspring). Stimuli related to copulation facilitate the first, whereas pregnancy, parturition and lactation facilitate the second. Both types develop as consequence of cohabitation. These events seem to stimulate similar neural pathways that increase (1) social recognition, (2) motivation, reward; and (3) decrease fear/anxiety. Subregions of the amygdala and cortex facilitate social recognition and also disinhibition to decrease rejection responses. The interrelationship between MeA, BNST, LS may mediate the activation of NAcc via the mPOA to increase motivation and reward. Cortical areas such as the ACC discriminate between stimuli. The interaction between OT and D2-type receptors in NAcc shell facilitates intraspecific attachment, but D1-type appears to facilitate parental attachment. This difference may be important for maternal females to direct their attention, motivation and expression of attachment toward the appropriate target.Cuerpo Académico UV-CA-28 Neurociencias. Grant SEP-CONACYT (167773) to GACA

Why should we keep the cerebellum in mind when thinking about addiction?

Increasing evidence has involved the cerebellum in functions beyond the sphere of motor control. In the present article, we review evidence that involves the cerebellum in addictive behaviour. We aimed on molecular and cellular targets in the cerebellum where addictive drugs can act and induce mechanisms of neuroplasticity that may contribute to the development of an addictive pattern of behaviour. Also, we analyzed the behavioural consequences of repetitive drug administration that result from activitydependent changes in the efficacy of cerebellar synapses. Revised research involves the cerebellum in drug-induced long-term memory, druginduced sensitization and the perseverative behavioural phenotype. Results agree to relevant participation of the cerebellum in the functional systems underlying drug addiction. The molecular and cellular actions of addictive drugs in the cerebellum involve long-term adaptative changes in receptors, neurotransmitters and intracellular signalling transduction pathways that may lead to the re-organization of cerebellar microzones and in turn to functional networks where the cerebellum is an important nodal structure. We propose that drug induced activity-dependent synaptic changes in the cerebellum are crucial to the transition from a pattern of recreational drug taking to the compulsive behavioural phenotype. Functional and structural modifications produced by drugs in the cerebellum may enhance the susceptibility of fronto-cerebellar circuitry to be changed by repeated drug exposure. As a part of this functional reorganization, drug-induced cerebellar hyper-responsiveness appears to be central to reducing the influence of executive control of the prefrontal cortex on behaviour and aiding the transition to an automatic mode of contro

Genetic Polymorphism in Evolving Population

We present a model for evolving population which maintains genetic polymorphism. By introducing random mutation in the model population at a constant rate, we observe that the population does not become extinct but survives, keeping diversity in the gene pool under abrupt environmental changes. The model provides reasonable estimates for the proportions of polymorphic and heterozygous loci and for the mutation rate, as observed in nature

The 0.1-200 keV spectrum of the blazar PKS 2005-489 during an active state

The bright BL Lac object PKS 2005-489 was observed by BeppoSAX on November 1-2, 1998, following an active X-ray state detected by RossiXTE. The source, detected between 0.1 and 200 keV, was in a very high state with a continuum well fitted by a steepening spectrum due to synchrotron emission only. Our X-ray spectrum is the flattest ever observed for this source. The different X-ray spectral slopes and fluxes, as measured by various satellites, are consistent with relatively little changes of the peak frequency of the synchrotron emission, always located below 10^{17} Hz. We discuss these results in the framework of synchrotron self-Compton models. We found that for the BeppoSAX observation, the synchrotron peak frequency is between 10^{15} and 2.5x10^{16} Hz, depending on the model assumptions.Comment: 7 pages, 4 figures, accepted for publication in A&

Amino acid tissue levels and GABAa receptor binding in the developing rat cerebellum following status epilepticus

Incidence of status epilepticus (SE) is higher in children than in adults and SE can be induced in developing rats. The cerebellum can be affected after SE; however, consequences of cerebellar amino acid transmission have been poorly studied. The goal of this study was to determine amino acid tissue concentration and GABAA receptor binding in the immature rat cerebellum after an episode of SE. Thirteen-day-old (P13) rat pups received in- traperitoneal injections of lithium chloride (3 mEq/kg). Twenty hours later, on P14, SE was induced by subcutaneous injection of pilocarpine hydrochloride (60 mg/kg). Control ani- mals were given an equal volume of saline subcutaneously. Animals were killed 24 h after SE induction, the cerebellum was quickly removed, and the vermis and hemispheres were rapidly dissected out on ice. Amino acid tissue concentrations in the vermis and hemi- spheres were evaluated by HPLC and fluorescent detection. GABAA receptor binding in the medial vermis was analyzed by in vitro autoradiography. SE increased the tissue levels of the inhibitory amino acids taurine (80%) and alanine (91%), as well as glutamine (168%) in the cerebellar hemisphere; no changes were observed in the vermis. SE did not modify GABAA receptor binding in any cerebellar lobule from the vermis. Our data demonstrate that SE produces region-specific changes in amino acid concentrations in the developing cerebellum

High redshift X-ray galaxy clusters. I. The impact of point sources on the cluster properties

The current generation of X-ray observatories like Chandra allows studies with very fine spatial details. It is now possible to resolve X-ray point sources projected into the cluster diffuse emission and exclude them from the analysis to estimate the ``correct'' X-ray observables. In order to verify the incidence of point sources on the cluster thermal emission and to evaluate the impact of their non-thermal emission on the determination of cluster properties, we used a sample of 18 high-z (0.25 < z < 1.01) clusters from the Chandra archive. We performed a detailed analysis of the cluster properties and compared the changes observed in the X-ray observables, like temperature and luminosity or their inter-relation, when one keeps the point sources in the analysis. The point sources projected into the cluster extended emission affect the estimates of cluster temperature or luminosity considerably (up to 13% and 17% respectively). These percentages become even larger for clusters with z > 0.7 where temperature and luminosity increase up to 24% and 22%, respectively. Thus the point sources should be removed to correctly estimate the cluster properties. However the inclusion of the point sources does not impact significantly the slope and normalization of the L-T relationship since for each cluster the correction to be applied to T and L produces a moderate shift in the L-T plane almost parallel to the best-fit of the ``correct'' L-T relation.Comment: 16 pages, 18 postscript figures. Accepted for publication in Astronomy & Astrophysic

Spectral Inversion of Multi-Line Full-Disk Observations of Quiet Sun Magnetic Fields

Spectral inversion codes are powerful tools to analyze spectropolarimetric observations, and they provide important diagnostics of solar magnetic fields. Inversion codes differ by numerical procedures, approximations of the atmospheric model, and description of radiative transfer. Stokes Inversion based on Response functions (SIR) is an implementation widely used by the solar physics community. It allows to work with different atmospheric components, where gradients of different physical parameters are possible, e.g., magnetic field strength and velocities. The spectropolarimetric full-disk observations were carried out with the Stokesmeter of the Solar Telescope for Operative Predictions (STOP) at the Sayan Observatory on 3 February 2009, when neither an active region nor any other extended flux concentration was present on the Sun. In this study of quiet Sun magnetic fields, we apply the SIR code simultaneously to 15 spectral lines. A tendency is found that weaker magnetic field strengths occur closer to the limb. We explain this finding by the fact that close to the limb, we are more sensitive to higher altitudes in an expanding flux tube, where the field strength should be smaller since the magnetic flux is conserved with height. Typically, the inversions deliver two populations of magnetic elements: (1) high magnetic field strengths (1500-2000 G) and high temperatures (5500-6500 K) and (2) weak magnetic fields (50-150 G) and low temperatures (5000-5300 K).Comment: 10 pages, 6 figures, accepted for Solar Physic