10,214 research outputs found

    Study of rotation curves of spiral galaxies with a scalar field dark matter model

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    In this work we study rotation curves of spiral galaxies using a model of dark matter based on a scalar-tensor theory of gravity. We show how to estimate the scalar field dark matter parameters using a sample of observed rotation curves.Comment: 6 pages, 2 figures. arXiv admin note: substantial text overlap with arXiv:1112.520

    Nucleic acids enrichment of fungal pathogens to study host-pathogen interactions

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    Fungal infections, ranging from superficial to life-threatening infections, represent a major public health problem that affects 25% of the worldwide population. In this context, the study of host-pathogen interactions within the host is crucial to advance antifungal therapy. However, since fungal cells are usually outnumbered by host cells, the fungal transcriptome frequently remains uncovered. We compared three different methods to selectively lyse human cells from in vitro mixes, composed of Candida cells and peripheral blood mononuclear cells. In order to prevent transcriptional modification, the mixes were stored in RNAlater. We evaluated the enrichment of fungal cells through cell counting using microscopy and aimed to further enrich fungal nucleic acids by centrifugation and by reducing contaminant nucleic acids from the host. We verified the enrichment of fungal DNA and RNA through qPCR and RT-qPCR respectively and confirmed that the resulting RNA has high integrity scores, suitable for downstream applications. The enrichment method provided here, i.e., lysis with Buffer RLT followed by centrifugation, may contribute to increase the proportion of nucleic acids from fungi in clinical samples, thus promoting more comprehensive analysis of fungal transcriptional profiles. Although we focused on C. albicans, the enrichment may be applicable to other fungal pathogens

    Los agujeros negros y las ondas del Doctor Einstein

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    We describe the main scientific developments that lead LIGO project to the detection of the gravitational waves: general relativity, black holes and gravitational waves predictions; numerical relativity and the collision and coalescence simulations of binary black holes and the development of different kind of gravitational wave detectors. Most important, this detection is confirming the existence of the enigmatic black holes.Comment: 9 pages, in Spanish, 2 figure

    Hydrometallurgical extraction of Al and Si from kaolinitic clays

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    Herein is presented the results of a study on the hydrometallurgic extractionand recovery of aluminum and silicon by leaching of kaolinitic clays with HF. Thestudied extraction parameters were: temperature, reaction time, solid/liquid ratio,concentration, and precipitating agent mass. In the leaching process, mineraldissolutions near 100% were obtained when working at 348 K, solid/liquid ratio2% w/v, HF 12% v/v, for 120 minutes. The HF leach liquor generated from thedissolution of kaolinitic clays contains H2SiF6 and H3AlF6. Studies were conductedto recover the two valuable fluorides as K2SiF6 and Na3AlF6 by precipitation withalkaline salts from the leach liquor. Phases of precipitated fluorides were identifiedby XRD and surface morphology by SEM. The purity of the K2SiF6 precipitate was98.8%, whereas for Na3AlF6, it was 89.3%. Also, both synthesized solids are ofhigh commercial value due to their industrial applications.Fil: Pinna, Eliana Guadalupe. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Suarez, Daniela Silvana. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Universidad Nacional de Cuyo; ArgentinaFil: Rosales, Gustavo Daniel. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; ArgentinaFil: Rodriguez, Mario Humberto. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza; Argentin
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