NUTRITIONAL ANALYSIS AND CHEMICAL FINGERPRINTING OF BLACK SOLDIER FLY (Hermetia illucens) LARVAE MEAL NATIVE TO THE PERUVIAN AMAZON

The search for new protein sources for food formulation is increasing. Black soldier fly (Hermetia illucens) larvae have high levels of protein, good fat content and are rich in essential amino acids, making them an alternative source of dietary protein. In this work, the nutritional properties and metabolomic characterization of black soldier fly larvae meal native to the Peruvian Amazon reared on three organic waste substrates were carried out. The three meals had a high protein content (38.90-44.68%), lipids (26.96-31.01%), as well as a high potassium, calcium and low sodium content. The larvae meal fed with orange bagasse presented the best content of iron and manganese. The fatty acid profile showed a high content of palmitic, oleic and linoleic acids for the three flours. The most abundant essential amino acids in the three meals were leucine, lysine and valine. A total of 48 compounds were identified in the larvae meal fed with orange bagasse, 24 compounds in the larvae meal fed with oats and 44 compounds in the larvae meal fed with ripe banana pulp. These results indicate that black soldier fly larvae meals native to the Peruvian Amazon are a good source of proteins, minerals, essential amino acids and unsaturated fatty acids with high nutritional value.La búsqueda de nuevas fuentes de proteínas para la formulación de alimentos es cada vez mayor. Las larvas de la mosca soldado negra (Hermetia illucens) tienen altos niveles de proteína, buen contenido de grasa y son ricas en aminoácidos esenciales, lo que las convierte en una fuente alternativa de proteína dietética. En este trabajo se evaluó las propiedades nutricionales mediante la composición proximal, contenido de minerales, perfil de ácidos grasos, aminoácidos y la caracterización metabolómica de harina de larvas de mosca soldado negra nativa de la amazonia peruana criadas en tres sustratos de residuos orgánicos. Las tres harinas presentaron un alto contenido de proteínas (38,90-44,68%), lípidos (26,96-31,01%), así como un alto contenido de potasio, calcio y bajo contenido en sodio. Las harinas de larvas alimentadas con el bagazo de naranja presentaron los mejores contenidos de hierro y manganeso. El perfil de ácidos grasos mostró un alto contenido los ácidos palmítico, oleico y linoleico para las tres harinas. Los amino ácidos esenciales más abundantes en las tres harinas fueron leucina, lisina y valina. Un total de 48 compuestos fueron identificados en la harina de larva alimentada con bagazo de naranja, 24 compuestos en la harina de larva alimentada con avena y 44 compuestos en la harina de larva alimentada con pulpa de plátano maduro. Estos resultados nos indican que las harinas de larva de mosca soldado negra nativa de la Amazonía peruana son una buena fuente de proteínas, minerales, aminoácidos esenciales y ácidos grasos insaturados con alto valor nutricional

Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient

The aim of this review is to provide overall information on Argentine propolis and to shed light on its potential, especially the one from the Monte region so as to support future research in the field. Around 1999, the Argentine propolis began to be chemically and functionally characterized to give it greater added value. Because Argentina has a wide plant biodiversity, it is expected that its propolis will have various botanical origins, and consequently, a different chemical composition. To date, five types have been defined. Based on their functionality, several products have been developed for use in human and veterinary medicine and in animal and human food. Because the Argentine propolis with the greatest potential is that of the Monte eco-region, this review will describe the findings of the last 20 years on this propolis, its botanical source (Zuccagnia punctata Cav.), its chemical composition, and a description of markers of chemical quality (chalcones) and functionality. Propolis can regulate the activity of various pro-inflammatory enzymes and carbohydrate and lipid metabolism enzymes, as well as remove reactive oxygen and nitrogen species. Consequently, it can modulate metabolic syndrome and could be used as a functional ingredient in food. Furthermore, hydroalcoholic extracts can act against human and animal pathogenic bacteria and human yeast, and mycelial pathogenic fungi. The ability to stop the growth of post-harvest pathogenic bacteria and fungi was also demonstrated. For this reason, Argentine propolis are natural products capable of protecting crops and increasing the lifespan of harvested fruit and vegetables. Several reports indicate the potential of Argentine propolis to be used in innovative products to improve health, food preservation, and packaging. However, there is still much to learn about these natural products to make a wholesome use of them.EEA FamailláFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina.Fil: Salas, Ana Lilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Salas, Ana Lilia. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Salas, Ana Lilia. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; ArgentinaFil: Maldonado, Luis Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Simirgiotis, Mario J. Universidad Austral de Chile. Facultad de Ciencias. Instituto de Farmacia; ChileFil: Isla, María Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Isla, María Inés. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Isla, María Inés. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina

Potencial antidiabético de plantas medicinales de la Amazonía peruana: Una revisión

La diabetes mellitus es un trastorno metabólico caracterizado por la presencia de hiperglucemia crónica acompañada, en mayor o menor medida, de alteraciones en el metabolismo de carbohidratos, proteínas y lípidos, es la principal causa de muerte en los países desarrollados. Los tratamientos actuales con fármacos anti-diabéticos presentan varios efectos adversos, por lo que se ha iniciado una búsqueda de nuevos hipoglucemiantes orales a partir de especies vegetales. Esta revisión presenta las plantas medicinales de la amazonia peruana utilizadas en el tratamiento de la diabetes y una revisión sistemática de sus propiedades hipoglucémicas reportadas en la literatura en los últimos veinte años utilizando bases de datos bibliográficas. Se reportan 77 plantas medicinales utilizadas para el tratamiento de la diabetes por pobladores amazónicos, de las cuales el 46.75% reporta actividad hipoglucémica, evidenciando que el conocimiento tradicional es una fuente para la búsqueda de fármacos antidiabéticos y una alternativa para futuras investigaciones.Revisión por pares

Potencial antidiabético de plantas medicinales de la amazonía peruana: Una revisión

Diabetes mellitus is a metabolic disorder characterized by the presence of chronic hyper-glycemia that is accompanied, to a greater or lesser extent, by alterations in the metabolism of carbohydrates, proteins and lipids. It is the leading cause of death in developed countries. Current antidiabetic drug treatments present several adverse effects for which it has started searching for new oral hypoglycemic agents from vegetal species. This review presents the medicinal plants from the Peruvian Amazon used in the treatment of diabetes and a systematic review of their hypoglycemic properties reported in the literature in the last twenty years used in different bibliographic databases. Seventy seven medicinal plants are reported as being used for the treatment of diabetes by Amazonian indigenous people, which 46.75% of species report hypoglycemic activity, evidencing that traditional knowledge is a great source for to searching antidiabetic drugs and also an alternative for future research.La diabetes mellitus es un trastorno metabólico caracterizado por la presencia de hiperglucemia crónica acompañada, en mayor o menor medida, de alteraciones en el metabolismo de carbohidratos, proteínas y lípidos, es la principal causa de muerte en los países desarrollados. Los tratamientos actuales con fármacos anti-diabéticos presentan varios efectos adversos, por lo que se ha iniciado una búsqueda de nuevos hipoglucemiantes orales a partir de especies vegetales. Esta revisión presenta las plantas medicinales de la amazonia peruana utilizadas en el tratamiento de la diabetes y una revisión sistemática de sus propiedades hipoglucémicas reportadas en la literatura en los últimos veinte años utilizando bases de datos bibliográficas. Se reportan 77 plantas medicinales utilizadas para el tratamiento de la diabetes por pobladores amazónicos, de las cuales el 46.75% reporta actividad hipoglucémica, evidenciando que el conocimiento tradicional es una fuente para la búsqueda de fármacos antidiabéticos y una alternativa para futuras investigaciones

Propiedades fisicoquímicas, análisis metabolómico, actividad antioxidante e hipolipemiante de una bebida funcional a base de cocona (Solanum sessiliflorum Dunal

The physicochemical, microbiological and metabolomics analysis, antioxidant and lipid-lowering effect, and shelf life prediction of a functional beverage based on cocona pulp of SRN9 ecotype was to carry out. According to the results obtained, the beverage complies with all the characteristics of the Peruvian technical standard for juices, nectars and fruit beverages NTP 203.110:2009 and is within the limits established by the sanitary technical standard NTS N° 071-MINSA/DIGESA-V.01, with a shelf-life period of 4 months and 1 day. The metabolome regarding bioactive compounds showed the presence of 30 compounds, including several glycosylated flavonols, two flavanols, and two spermidines. Likewise, showed a lipid-lowering effect statistically significant (p < 0.05) about the serum levels of total cholesterol and triglycerides, with a mean reduction of 41.52 mg/dL for total cholesterol levels and 130.80 mg/dL for triglyceride levels. This beverage could be an alternative for the treatment of atherosclerosis and prevention of cardiovascular diseases.Se realizó el análisis fisicoquímico, microbiológico y metabolómico, efecto antioxidante e hipolipemiante, y vida útil de una bebida funcional a base de cocona ecotipo SRN9. De acuerdo a los resultados, la bebida cumple con las características de la norma técnica peruana para jugos, néctares y bebidas de frutas NTP 203.110:2009 y se encuentra dentro de los límites establecidos por la norma técnica sanitaria NTS N° 071-MINSA/DIGESA-V.01, con una vida útil de 4 meses y 1 día. Del perfil metabolómico se identificaron 30 compuestos, entre ellos varios flavonoles glicosilados, dos flavanoles y dos espermidinas. Asimismo, mostró un efecto hipolipemiante estadísticamente significativo (p < 0,05) sobre los niveles séricos de colesterol total y triglicéridos, con una reducción media de 41,52 mg/dL y de 130,80 mg/dL para los niveles de colesterol total y de triglicéridos, respectivamente. Esta bebida podría ser una alternativa para el tratamiento de la aterosclerosis y prevención de enfermedades cardiovasculares

UHPLC–Q/Orbitrap/MS/MS fingerprinting, free radical scavenging and antimicrobial activity of tessaria absinthiodes (Hook. & Arn.) DC. (Asteraceae) lyophilized decoction from Argentina and Chile

The decoction of Tessaria absinthioides is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC–PDA–OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC50 = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86–88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC–PDA–OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health.Fil: Gómez, Jessica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Simirgiotis, Mario Juan. Universidad Austral de Chile. , Facultad de Ciencias. Instituto de Farmacia; Chile. Universidad Austral de Chile. Center for Interdisciplinary Studies on the Nervous System; ChileFil: Lima, Beatriz. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gamarra Luques, Carlos Diego. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Bórquez, Jorge. Universidad de Antofagasta; ChileFil: Caballero, Duilio. Hospital Marcial Quiroga; ArgentinaFil: Feresin, Gabriela Egly. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; ArgentinaFil: Tapia, Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería; Argentin

Zuccagnia punctata Cav, a Potential Environmentally Friendly and Sustainable Bionematicide for the Control of Argentinean Horticultural Crops

This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential nematicidal properties of decoction (ZpDe), orange-yellow resin (ZpRe) and essential oil (ZpEO) from Argentinean medicinal plant Zuccagnia punctata Cav. Additionally, the antioxidant and antibacterial properties of ZpDe and ZpEO were determined. Metabolite profiling was obtained by an ultrahigh-resolution liquid chromatography MS analysis (UHPLC-ESI-QTOF/OT-MS-MS) and GCMS. The nematicidal activity was assayed by a standardized method against Meloidogyne incognita. The antioxidant properties were screened by four methods: (2,2-diphenyl-1-picrylhydrazyl assay (DPPH), Trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP), and lipid peroxidation in erythrocytes (ILP). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The ZpDe, ZpRe and ZpEO displayed a strong nematicidal activity with an LC50 of 0.208, 0.017 and 0.142 mg/mL, respectively. On the other hand, the ZpDe showed a strong DPPH scavenging activity (IC50 = 28.54 µg/mL); ILP of 87.75% at 250 µg ZpDe/mL and moderated antimicrobial activity. The ZpEO showed promising activity against a panel of yeasts Candida albicans and non-albicans (ATCC and clinically isolated) with MIC values from 750 to 1500 µg/mL. The ZpDe showed a content of phenolics and flavonoid compounds of 241 mg GAE/g and 10 mg EQ/g, respectively. Fifty phenolic compounds were identified in ZpDe by ultrahigh-resolution liquid chromatography (UHPLC–PDA– Q-TOF-MS) analysis, while forty-six phenolic compounds were identified in ZpRe by UHPLC-ESI-Q-OT-MS-MS and twenty-nine in ZpEO using a GC-MS analysis, updating the knowledge on the chemical profile of this species. The results support and standardize this medicinal plant mainly as a potential environmentally friendly and sustainable bionematicide for the control of Argentinean horticultural crops including tomatoes and peppers and as a source of antimicrobial and antioxidant compounds which could be further explored and exploited for potential applications

Antifungal activity of phytotherapeutic preparation of Baccharis species from argentine Puna against clinically relevant fungi

Ethnopharmacological relevance: B. boliviensis and B. tola are used in traditional medicine in the Argentine Puna to treat skin and soft tissue infections and inflammatory processes in humans and animals. Aim of the study: To assess the potential of phytotherapeutic preparations of Baccharis species as antifungal agents against clinically relevant fungi and to determine the chemical composition of the extracts. Material and methods: Phytotherapeutic preparations of B. boliviensis and B. tola collected in Argentine Puna were evaluated as an antifungal agent against clinically relevant fungi (yeast, non-dermatophytes, and dermatophytes) isolated of patients from a local Hospital, and reference strains, using macrodilution and microdilution assays. The bioactivity was supported by UHPLC-OT-MS metabolome fingerprinting. Results: The results revealed that the plant preparations were active against most of evaluated fungal strains; B. boliviensis was more active than B. tola. Dermatophyte fungi strains were the most sensitive isolates. The phytotherapeutic preparation showed Minimal Inhibitory Concentration (MIC) values between 25 and 400 μg GAE/mL and Minimum Fungicidal Concentration (MFC) values between 50 and 400 μg GAE/mL. Regarding the phytochemical analysis, total phenolic and total flavonoid contents of hydroalcoholic preparation of B. boliviensis were greater than those of the B. tola extract. Both Baccharis species showed similar chromatographic patterns, fifty-two compounds were identified based on UHPLC-OT-MS including several terpenoids, flavonoids and phenolic acids that have been identified in this two endemic South American Baccharis species for the first time. Several identified compounds present antifungal properties, the presence of these compounds support the bioactivity of the Baccharis extracts. Conclusions: In this work the traditional use of both Baccharis species as an antimicrobial against commercial products resistant fungal strains was validate, principally against dermatophytes fungi such as T. rubrum, T. mentagrophytes, M. canis, and M. gypseum. These results indicate that the hydroalcoholic preparations could be used for the treatment of fungal infectious.Fil: Carrizo, Silvana Lorena. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; ArgentinaFil: Zampini, Iris Catiana. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Sayago, Jorge Esteban. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Simirgiotis, Mario Juan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Austral de Chile; ChileFil: Bórquez, Jorge. Universidad de Antofagasta. Facultad de Ciencias Básica. Departamento de Química. Laboratorio de Productos Naturales; ChileFil: Cuello, Ana Soledad. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentin

UHPLC-HESI-OT-MS-MS biomolecules profiling, antioxidant and antibacterial activity of the “orange-yellow resin” from zuccagnia punctata Cav

This research was designed to investigate the metabolite profiling, phenolics, and flavonoids content as well as the potential antioxidant and antibacterial, properties of orange-yellow resin from Zuccagnia punctata Cav (ZpRe). Metabolite profiling was obtained by a ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS-MS). The antioxidant properties were screened by four methods: 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP), and lipid peroxidation in erythrocytes (LP)). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) rules. The resin displayed a strong DPPH scavenging activity (IC50 = 25.72 µg/mL) and showed a percentage of inhibition of LP close to that of the reference compound catechin (70% at 100 µg ZpRe/mL), while a moderated effect was observed in the FRAP and TEAC assays. The resin showed a content of phenolic and flavonoid compounds of 391 mg GAE/g and 313 mg EQ/g respectively. Fifty phenolics compounds were identified by ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-PDA-OT-MS) analysis. Thirty-one compounds are reported for the first time, updating the knowledge on the chemical profile of this species. The importance of the biomolecules identified support traditional use of this endemic plant. Furthermore, additional pharmacological data is presented that increase the potential interest of this plant for industrial sustainable applications.Fil: Gómez Pelaytay, Jessica Belén. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Simirgiotis, Mario Juan. Universidad Austral de Chile; Chile. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Manrique, Sofia. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Lima, Beatriz Viviana. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Bórquez, Jorge. Universidad de Antofagasta; ChileFil: Feresin, Gabriela Egly. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Juan; ArgentinaFil: Tapia, Aníbal Alejandro. Universidad Nacional de San Juan. Facultad de Ingeniería. Instituto de Biotecnología; Argentin

Anthocyanin Characterization, Total Phenolic Quantification and Antioxidant Features of Some Chilean Edible Berry Extracts

The anthocyanin composition and HPLC fingerprints of six small berries endemic of the VIII region of Chile were investigated using high resolution mass analysis for the first time (HR-ToF-ESI-MS). The antioxidant features of the six endemic species were compared, including a variety of blueberries which is one of the most commercially significant berry crops in Chile. The anthocyanin fingerprints obtained for the fruits were compared and correlated with the antioxidant features measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP), the superoxide anion scavenging activity assay (SA), and total content of phenolics, flavonoids and anthocyanins measured by spectroscopic methods. Thirty one anthocyanins were identified, and the major ones were quantified by HPLC-DAD, mostly branched 3-O-glycosides of delphinidin, cyanidin, petunidin, peonidin and malvidin. Three phenolic acids (feruloylquinic acid, chlorogenic acid, and neochlorogenic acid) and five flavonols (hyperoside, isoquercitrin, quercetin, rutin, myricetin and isorhamnetin) were also identified. Calafate fruits showed the highest antioxidant activity (2.33 ± 0.21 μg/mL in the DPPH assay), followed by blueberry (3.32 ± 0.18 μg/mL), and arrayán (5.88 ± 0.21), respectively