Exact properties of Efron's biased coin randomization procedure

Efron [Biometrika 58 (1971) 403--417] developed a restricted randomization procedure to promote balance between two treatment groups in a sequential clinical trial. He called this the biased coin design. He also introduced the concept of accidental bias, and investigated properties of the procedure with respect to both accidental and selection bias, balance, and randomization-based inference using the steady-state properties of the induced Markov chain. In this paper we revisit this procedure, and derive closed-form expressions for the exact properties of the measures derived asymptotically in Efron's paper. In particular, we derive the exact distribution of the treatment imbalance and the variance-covariance matrix of the treatment assignments. These results have application in the design and analysis of clinical trials, by providing exact formulas to determine the role of the coin's bias probability in the context of selection and accidental bias, balancing properties and randomization-based inference.Comment: Published in at http://dx.doi.org/10.1214/09-AOS758 the Annals of Statistics (http://www.imstat.org/aos/) by the Institute of Mathematical Statistics (http://www.imstat.org

Several specific and nonspecific responses of the human and animal body to ship noise

The effect of noise on cargo boats on a long voyage differs considerably from the effect of noise in factories and in service industries. The peculiarities of the effect of round-the-clock noises at sea at 55 to 85 decibels, typical for cargo boats, were studied in white rats in the laboratory and aboard ship (each of the experiments lasted three months) and in young naval cadets and experienced seamen on voyages lasting one, two, and three months. The findings helped to derive health standards for maximum admissible noise level at sea

Improved osteogenic vector for non-viral gene therapy

Therapeutic compensation of deficient bone regeneration is a challenging task and a topic of on-going search for novel treatment strategies. One promising approach for improvement involves non-viral gene delivery using the bone morphogenetic protein-2 (BMP-2) gene to provide transient, local and sustained expression of the growth factor. However, since efficiency of non-viral gene delivery is low, this study focused on the improvement of a BMP-2 gene expression system, aiming for compensation of poor transfection efficiency. First, the native BMP-2 gene sequence was modified by codon optimisation and altered by inserting a highly truncated artificial intron (96 bp). Transfection of multiple cell lines and rat adipose-derived mesenchymal stem cells with plasmids harbouring the improved BMP-2 sequence led to a several fold increased expression rate and subsequent osteogenic differentiation. Additionally, comparing expression kinetics of elongation factor 1 alpha (EF1α) promoter with a state of the art CMV promoter revealed significantly higher BMP-2 expression when under the influence of the EF1α promoter. Results obtained by quantification of bone markers as well as osteogenic assays showed reduced sensitivity to promoter silencing effects of the EF1α promoter in rat adipose-derived mesenchymal stem cells. Finally, screening of several protein secretion signals using either luciferase or BMP-2 as reporter protein revealed no superior candidates for potential replacement of the native BMP-2 secretion signal. Taken together, by enhancing the exogenous BMP-2 expression system, low transfection efficiencies in therapeutic applications can be compensated, making safe non-viral systems even more suitable for tissue regeneration approaches

Neki pokazatelji faznog prijelaza tekućina-plin u razbijanju jezgri 197Au elektronima

The energy spectra and fragment yields (5&lt;ZF&lt;10) obtained in 1.5 - 4.5 GeV e + 197Au inclusive reactions are analysed. Based on the observed correlation between a decrease in the fragment yields and an increase in the fragment Coulomb energy with the fragment charge, temperatures of the fragmenting system are derived. It is shown that the fragment yields exhibit the feature of thermal scaling in accordance with the Fisher\u27s droplet model. By using this model, values for the zero temperature surface energy coefficient, c0=11.8±0.8 MeV, the critical exponent, , σ = 0.56±0.03, as well as estimation of the critical temperature, Tc ≈ 18 MeV are obtained. The obtained results argue in favour of nuclear liquid-gas phase transition in these electron-induced reactions.Analiziramo energijske spektre i prinose izbačenih jezgri (5 < ZF < 10) u inkluzivnim reakcijama 1.5 – 4.5 GeV e + 197Au. Na osnovi opaženih korelacija smanjenja prinosa izbačenih jezgri i povećanja Coulombove energije s nabojem izbačenih jezgri izvodimo temperature udarenog sustava. Opažamo da prinosi izbačenih jezgri pokazuju toplinsko sumjeravanje u skladu s Fisherovim modelom kapljice. Primjenom tog modela izveli smo koeficijent površinske energije na temperaturnoj nuli, c0 = 11.8 ± 0.8 MeV, i kritični eksponent, σ = 0.56 ± 0.03, te ocijenili kritičnu temperaturu, Tc ≈ 18 MeV. Postignuti rezultati ukazuju na nuklearni fazni prijelaz tekućina-plin u tim reakcijama izazvanim elektronim

The Updated Zwicky Catalog (UZC)

The Zwicky Catalog of galaxies (ZC), with m_Zw<=15.5mag, has been the basis for the Center for Astrophysics (CfA) redshift surveys. To date, analyses of the ZC and redshift surveys based on it have relied on heterogeneous sets of galaxy coordinates and redshifts. Here we correct some of the inadequacies of previous catalogs by providing: (1) coordinates with <~2 arcsec errors for all of the Nuzc catalog galaxies, (2) homogeneously estimated redshifts for the majority (98%) of the data taken at the CfA (14,632 spectra), and (3) an estimate of the remaining "blunder" rate for both the CfA redshifts and for those compiled from the literature. For the reanalyzed CfA data we include a calibrated, uniformly determined error and an indication of the presence of emission lines in each spectrum. We provide redshifts for 7,257 galaxies in the CfA2 redshift survey not previously published; for another 5,625 CfA redshifts we list the remeasured or uniformly re-reduced value. Among our new measurements, Nmul are members of UZC "multiplets" associated with the original Zwicky catalog position in the coordinate range where the catalog is 98% complete. These multiplets provide new candidates for examination of tidal interactions among galaxies. All of the new redshifts correspond to UZC galaxies with properties recorded in the CfA redshift compilation known as ZCAT. About 1,000 of our new measurements were motivated either by inadequate signal-to-noise in the original spectrum or by an ambiguous identification of the galaxy associated with a ZCAT redshift. The redshift catalog we include here is ~96% complete to m_Zw<=15.5, and ~98% complete (12,925 galaxies out of a total of 13,150) for the RA(1950) ranges [20h--4h] and [8h--17h] and DEC(1950) range [-2.5d--50d]. (abridged)Comment: 34 pp, 7 figs, PASP 1999, 111, 43