A critical view on abc transporters and their interacting partners in auxin transport

Different subclasses of ATP-binding cassette (ABC) transporters have been implicated in the transport of native variants of the phytohormone auxin. Here, the putative, individual roles of key members belonging to the ABCB, ABCD and ABCG families, respectively, are highlighted and the knowledge of their assumed expression and transport routes is reviewed and compared with their mutant phenotypes. Protein–protein interactions between ABC transporters and regulatory components during auxin transport are summarized and their importance is critically discussed. There is a focus on the functional interaction between members of the ABCB family and the FKBP42, TWISTED DWARF1, acting as a chaperone during plasma membrane trafficking of ABCBs. Further, the mode and relevance of functional ABCB-PIN interactions is diagnostically re-evaluated. A new nomenclature describing precisely the most likely ABCB–PIN interaction scenarios is suggested. Finally, available tools for the detection and prediction of ABC transporter interactomes are summarized and the potential of future ABC transporter interactome maps is highlighted

DNA methylation at the mu-1 opioid receptor gene (OPRM1) promoter predicts preoperative, acute, and chronic postsurgical pain after spine fusion.

INTRODUCTION:The perioperative pain experience shows great interindividual variability and is difficult to predict. The mu-1 opioid receptor gene (OPRM1) is known to play an important role in opioid-pain pathways. Since deoxyribonucleic acid (DNA) methylation is a potent repressor of gene expression, DNA methylation was evaluated at the OPRM1 promoter, as a predictor of preoperative, acute, and chronic postsurgical pain (CPSP). METHODS:A prospective observational cohort study was conducted in 133 adolescents with idiopathic scoliosis undergoing spine fusion under standard protocols. Data regarding pain, opioid consumption, anxiety, and catastrophizing (using validated questionnaires) were collected before and 2-3 months postsurgery. Outcomes evaluated were preoperative pain, acute postoperative pain (area under curve [AUC] for pain scores over 48 hours), and CPSP (numerical rating scale >3/10 at 2-3 months postsurgery). Blood samples collected preoperatively were analyzed for DNA methylation by pyrosequencing of 22 CpG sites at the OPRM1 gene promoter. The association of each pain outcome with the methylation percentage of each CpG site was assessed using multivariable regression, adjusting for significant (P<0.05) nongenetic variables. RESULTS:Majority (83%) of the patients reported no pain preoperatively, while CPSP occurred in 36% of the subjects (44/121). Regression on dichotomized preoperative pain outcome showed association with methylation at six CpG sites (1, 3, 4, 9, 11, and 17) (P<0.05). Methylation at CpG sites 4, 17, and 18 was associated with higher AUC after adjusting for opioid consumption and preoperative pain score (P<0.05). After adjusting for postoperative opioid consumption and preoperative pain score, methylation at CpG sites 13 and 22 was associated with CPSP (P<0.05). DISCUSSION:Novel CPSP biomarkers were identified in an active regulatory region of the OPRM1 gene that binds multiple transcription factors. Inhibition of binding by DNA methylation potentially decreases the OPRM1 gene expression, leading to a decreased response to endogenous and exogenous opioids, and an increased pain experience

Plant development regulated by cytokinin sinks

Morphogenetic signals control the patterning of multicellular organisms. Cytokinins are mobile signals that are perceived by subsets of plant cells. We found that the responses to cytokinin signaling during Arabidopsis development are constrained by the transporter PURINE PERMEASE 14 (PUP14). In our experiments, the expression of PUP14 was inversely correlated to the cytokinin signaling readout. Loss of PUP14 function allowed ectopic cytokinin signaling accompanied by aberrant morphogenesis in embryos, roots, and the shoot apical meristem. PUP14 protein localized to the plasma membrane and imported bioactive cytokinins, thus depleting apoplastic cytokinin pools and inhibiting perception by plasma membrane–localized cytokinin sensors to create a sink for active ligands. We propose that the spatiotemporal cytokinin sink patterns established by PUP14 determine the cytokinin signaling landscape that shapes the morphogenesis of land plants

First Chemical Analysis of Stars in The Triangulum-Andromeda Star Cloud

We undertake the first high resolution spectroscopic study of the Triangulum-Andromeda (TriAnd) star cloud --- an extended, mid-latitude Milky Way halo substructure about 20 kpc away in the second Galactic quadrant --- through six M giant star candidates selected to be both spatially and dynamically associated with this system. The abundance patterns of [Ti/Fe], [Y/Fe] and [La/Fe] as a function of [Fe/H] for these stars support TriAnd as having an origin in a dwarf galaxy with a chemical enrichment history somewhat similar to that of the Sagittarius dwarf spheroidal (dSph) galaxy. We also investigate the previously proposed hypothesis that TriAnd is an outlying, dynamically older piece of the Monoceros Stream (also known as the Galactic Anticenter Stellar Structure, "GASS") under the assumption that both features come from the tidal disruption of the same accreted Milky Way satellite and find that net differences in the above abundance patterns between the TriAnd and GASS stars studied suggest that these two systems are independent and unrelated.Comment: Accepted for publication in ApJ Letter

Topology and Thermophoresis Characterization of Complex Polymers by Thermal Field-Flow Fractionation

This dissertation deals with the potential of thermal field-flow fractionation (ThFFF) for the multidetection-based analysis of polymers with complex topology to prove its capability in resolving polymer branching characteristics from measured thermophoretic properties. For that, not yet existent but necessary profound advances in the theory of ThFFF and as well, thermophoresis of branched polymers were generated to allow a full exploitation of the method in the elucidation of polymer topology. Exemplary, two different libraries of branched polymer model systems based on aliphatic-aromatic polyesters and on a new type of short chain branched polyethylene were investigated. On top, the potential of the optimized ThFFF theory was assessed in the context of crosslinked polymer architectures and shines light onto the so far controversially debated topic of electron beam irradiation effects on thermoplastic polyurethane

SHADE AVOIDANCE 4 is required for proper auxin distribution in the hypocotyl

The phytohormone auxin is involved in virtually every aspect of plant growth and development. Through polar auxin transport, auxin gradients can be established, which then direct plant differentiation and growth. Shade avoidance responses are well- known processes that require polar auxin transport. In this study, we have identified a mutant, shade avoidance 4 (sav4), defective in shade-induced hypocotyl elongation and basipetal auxin transport. SAV4 encodes an unknown protein with armadillo repeat- and tetratricopeptide repeat-like domains known to provide protein-protein interaction surfaces. C terminally yellow fluorescent protein-tagged SAV4 localizes to both the plasma membrane and the nucleus. Membrane-localized SAV4 displays a polar association with the shootward plasma membrane domain in hypocotyl and root cells, which appears to be necessary for its function in hypocotyl elongation. Cotransfection of SAV4 and ATP-binding cassette B1 (ABCB1) auxin transporter in tobacco (Nicotiana benthamiana) revealed that SAV4 blocks ABCB1-mediated auxin efflux. We thus propose that polarly localized SAV4 acts to inhibit ABCB-mediated auxin efflux toward shoots and facilitates the establishment of proper auxin gradients

High Resolution HDS/SUBARU chemical abundances of the young stellar cluster Palomar 1

Context. Palomar\,1 is a peculiar globular cluster (GC). It is the youngest Galactic GC and it has been tentatively associated to several of the substructures recently discovered in the Milky Way (MW), including the Canis Major (CMa) overdensity and the Galactic Anticenter Stellar Structure (GASS). Aims. In order to provide further insights into its origin, we present the first high resolution chemical abundance analysis for one red giant in Pal\,1. Methods. We obtained high resolution (R=30000) spectra for one red giant star in Pal\,1 using the High Dispersion Spectrograph (HDS) mounted at the SUBARU telescope. We used ATLAS-9 model atmospheres coupled with the SYNTHE and WIDTH calculation codes to derive chemical abundances from the measured line equivalent widths of 18 among $\alpha$, Iron-peak, light and heavy elements. Results. The Palomar~1 chemical pattern is broadly compatible to that of the MW open clusters population and similar to disk stars. It is, instead, remarkably different from that of the Sagittarius (Sgr) dwarf spheroidal galaxy. Conclusions. If Pal\,1 association with either CMa or GASS will be confirmed, this will imply that these systems had a chemical evolution similar to that of the Galactic disk.Comment: 13 pages, 9 figures, 5 tables. Accepted for publication in Astronomy and Astrophysics. Minor changes in the tex

Social policies, separation and second birth spacing in Western Europe

The research leading to these results received funding from the European Union’s Seventh Framework Programme (FP7/2007–2013) under grant agreement no. 320116 for the research project FamiliesAndSocieties. We also acknowledge funds from the Academy of Finland (decision number 275030 and 293103) and the German Science Foundation (KR 2855/3–1 and TR 457/7–1).Objective: This paper studies postseparation fertility behavior. The aim is to investigate whether, and if so how, separation affects second birth spacing in Western European countries. Methods: This analysis makes use of rich survey data from Belgium, France, Germany, Italy, Spain, and the United Kingdom, as well as from Finnish register data. We thus cover the behavior of a large proportion of the population of Western Europe. We also use descriptive measures, such as Kaplan‒Meier survival functions and cumulative incidence curves. In the multivariate analysis, we employ event history modeling to show how education relates to postseparation fertility behavior. Results: There are large differences in postseparation fertility behavior across European countries. For Spain and Italy, we find that only a negligibly small proportion of the population have a second child after separating from the other parent of the firstborn child. The countries with the highest proportion of second children with a new partner are the United Kingdom, Germany, and Finland. In all countries, separation after first birth leads to a sharp increase in the birth interval between first and second births. Contribution: Our study is a contribution to the demographic literature that aims at understanding birth spacing patterns in Western Europe. Furthermore, we draw attention to the role of postseparation policies in explaining country differences in fertility behavior in contemporary societies.Publisher PDFPeer reviewe

A Wide Field Survey of Satellite Galaxies around the Spiral Galaxy M106

We present a wide field survey of satellite galaxies in M106 (NGC 4258) covering a 1.7\degr \times 2\degr field around M106 using Canada-France-Hawaii Telescope/MegaCam. We find 16 satellite galaxy candidates of M106. Eight of these galaxies are found to be dwarf galaxies that are much smaller and fainter than the remaining galaxies. Four of these galaxies are new findings. Surface brightness profiles of 15 out of 16 satellite galaxies can be represented well by an exponential disk profile with varying scale length. We derive the surface number density distribution of these satellite galaxies. The central number density profile (d $<100$ kpc) is well fitted by a power-law with a power index of $-2.1\pm0.5$, similar to the expected power index of isothermal distribution. The luminosity function of these satellites is represented well by the Schechter function with a faint end slope of $-1.19^{+0.03}_{-0.06}$. Integrated photometric properties (total luminosity, total colour, and disk scale length) and the spatial distribution of these satellite galaxies are found to be roughly similar to those of the Milky Way and M31.Comment: Accepted by MNRA

Chemical Signatures of the First Galaxies: Criteria for One-Shot Enrichment

We utilize metal-poor stars in the local, ultra-faint dwarf galaxies (UFDs; L_tot < 10^5 L_sun) to empirically constrain the formation process of the first galaxies. Since UFDs have much simpler star formation histories than the halo of the Milky Way, their stellar populations should preserve the fossil record of the first supernova (SN) explosions in their long-lived, low-mass stars. Guided by recent hydrodynamical simulations of first galaxy formation, we develop a set of stellar abundance signatures that characterize the nucleosynthetic history of such an early system if it was observed in the present-day universe. Specifically, we argue that the first galaxies are the product of chemical "one-shot" events, where only one (long-lived) stellar generation forms after the first, Population III, SN explosions. Our abundance criteria thus constrain the strength of negative feedback effects inside the first galaxies. We compare the stellar content of UFDs with these one-shot criteria. Several systems (Ursa Major II, and also Coma Berenices, Bootes I, Leo IV, Segue 1) largely fulfill the requirements, indicating that their high-redshift predecessors did experience strong feedback effects that shut off star formation. We term the study of the entire stellar population of a dwarf galaxy for the purpose of inferring details about the nature and origin of the first galaxies "dwarf galaxy archaeology". This will provide clues to the connection of the first galaxies, the surviving, metal-poor dwarf galaxies, and the building blocks of the Milky Way.Comment: 9 pages (emulateapj), 2 figures, ApJ in pres