Lokiarchaeon is hydrogen dependent

The nature of host that acquired the mitochondrion at eukaryote origin (the host) is an important microbial evolutionary issue. Modern phylogenetics indicate that the host was an archaeon. The metagenome sequence of Candidatus Lokiarchaeon identified it as the closest relative of the host yet known. Here we report comparative genomic evidence indicating that Lokiarchaeaon is hydrogen-dependent, as one theory for eukaryote origin — the hydrogen hypothesis — predicted for the host lineage

Abschlussbericht

Herzinsuffizienz (HF) ist ein globales Gesundheitsproblem, das erhebliche Auswirkungen auf die Lebensqualität der Betroffenen hat. Ziel dieser Studie ist es, die potenziellen Vorteile einer frühen Integration der Palliativversorgung (EIPC) bei Herzinsuffizienzpatienten zu untersuchen, wobei der Schwerpunkt auf der Lebensqualität liegt und der Vergleich zur bestmöglichen kardiologischen Versorgung (Best Support Cardiac Care, BSCC) erfolgt. Datei-Upload durch TIBHeart failure (HF) is a global health concern associated with a significant impact on patients' quality of life. This study aims to assess the potential benefits of early integration of palliative care (EIPC) in HF patients, focusing on health status and mood, as compared to best support cardiac care (BSCC)

Local and global Cdc42 GEFs for fission yeast cell polarity are coordinated by microtubules and the Tea1/Tea4/Pom1 axis

The conserved Rho-family GTPase Cdc42 plays a central role in eukaryotic cell polarity. The rod-shaped fission yeast Schizosaccharomyces pombe has two Cdc42 guanine nucleotide exchange factors (GEFs), Scd1 and Gef1, but little is known about how they are coordinated in polarized growth. Although the microtubule cytoskeleton is normally not required for polarity maintenance in fission yeast, we show here that when scd1 function is compromised, disruption of microtubules or the polarity landmark proteins Tea1, Tea4 or Pom1 leads to disruption of polarized growth. Instead, cells adopt an isotropic-like pattern of growth, which we term PORTLI growth. Surprisingly, PORTLI growth is caused by spatially inappropriate activity of Gef1. Although most Cdc42 GEFs are membrane associated, we find that Gef1 is a broadly distributed cytosolic protein rather than a membrane-associated protein at cell tips like Scd1. Microtubules and the Tea1–Tea4–Pom1 axis counteract inappropriate Gef1 activity by regulating the localization of the Cdc42 GTPase-activating protein Rga4. Our results suggest a new model of fission yeast cell polarity regulation, involving coordination of ‘local’ (Scd1) and ‘global’ (Gef1) Cdc42 GEFs via microtubules and microtubule-dependent polarity landmarks