Thermal constraints on in vivo optogenetic manipulations.

A key assumption of optogenetics is that light only affects opsin-expressing neurons. However, illumination invariably heats tissue, and many physiological processes are temperature-sensitive. Commonly used illumination protocols increased the temperature by 0.2-2 °C and suppressed spiking in multiple brain regions. In the striatum, light delivery activated an inwardly rectifying potassium conductance and biased rotational behavior. Thus, careful consideration of light-delivery parameters is required, as even modest intracranial heating can confound interpretation of optogenetic experiments

Computer simulations of cosmic-ray diffusion near supernova remnant shock waves

A plasma simulation model was used to study the resonant interactions between streaming cosmic-ray ions and a self-consistent spectrum of Alfven waves, such as might exist in the interstellar medium upstream of a supernova remnant shock wave. The computational model is a hybrid one, in which the background interstellar medium is an MHD fluid and the cosmic-rays are discrete kinetic particles. The particle sources for the electromagnetic fields are obtained by averaging over the fast cyclotron motions. When the perturbed magnetic field is larger than 10 percent of the background field, the macro- and microphysics are no longer correctly predicted by quasi-linear theory. The particles are trapped by the waves and show sharp jumps in their pitch-angles relative to the background magnetic field, and the effective ninety-degree scattering time for diffusion parallel to the background magnetic field is reduced to between 5 and 30 cyclotron periods. Simulation results suggest that Type 1 supernova remnants may be the principal sites of cosmic ray acceleration

The Connection Between Reddening, Gas Covering Fraction, and the Escape of Ionizing Radiation at High Redshift

We use a large sample of galaxies at z~3 to establish a relationship between reddening, neutral gas covering fraction (fcov(HI)), and the escape of ionizing photons at high redshift. Our sample includes 933 galaxies at z~3, 121 of which have very deep spectroscopic observations (>7 hrs) in the rest-UV (lambda=850-1300 A) with Keck/LRIS. Based on the high covering fraction of outflowing optically-thick HI indicated by the composite spectra of these galaxies, we conclude that photoelectric absorption, rather than dust attenuation, dominates the depletion of ionizing photons. By modeling the composite spectra as the combination of an unattenuated stellar spectrum including nebular continuum emission with one that is absorbed by HI and reddened by a line-of-sight extinction, we derive an empirical relationship between E(B-V) and fcov(HI). Galaxies with redder UV continua have larger covering fractions of HI characterized by higher line-of-sight extinctions. Our results are consistent with the escape of Lya through gas-free lines-of-sight. Covering fractions based on low-ionization interstellar absorption lines systematically underpredict those deduced from the HI lines, suggesting that much of the outflowing gas may be metal-poor. We develop a model which connects the ionizing escape fraction with E(B-V), and which may be used to estimate the escape fraction for an ensemble of high-redshift galaxies. Alternatively, direct measurements of the escape fraction for our data allow us to constrain the intrinsic 900-to-1500 A flux density ratio to be >0.20, a value that favors stellar population models that include weaker stellar winds, a flatter initial mass function, and/or binary evolution. Lastly, we demonstrate how the framework discussed here may be used to assess the pathways by which ionizing radiation escapes from high-redshift galaxies. [Abridged]Comment: 22 pages, 3 tables, 14 figures, accepted to the Astrophysical Journa